Amorphous magnesium phosphate-graphene oxide nano particles laden 3D-printed chitosan scaffolds with enhanced osteogenic potential and antibacterial properties.

The utilization of 3D printing technology for the fabrication of graft substitutes in bone repair holds immense promise. However, meeting the requirements for printability, bioactivity, mechanical strength, and biological properties of 3D printed structures concurrently poses a significant challenge. In this study, we introduce a novel approach by incorporating amorphous magnesium phosphate-graphene oxide (AMP-GO) into a thermo-crosslinkable chitosan/ß glycerol phosphate (CS/GP) ink. We fabricated thermo-crosslinkable CS inks containing varying concentrations (10 %, 20 %, or 30 % weight) of AMP-GO. The 3D printed scaffolds incorporating 20 % AMP-GO exhibited significantly improved mechanical properties, with compressive strengths of 4.5 ± 0.06 MPa compared to 0.5 ± 0.03 MPa for CS printed scaffolds. Moreover, the CS/AMP-GO inks demonstrated enhanced antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria, attributed to the release of magnesium cations and the performance of GO. Additionally, CS/20AMP-GO ink facilitated increased adhesion, viability, proliferation, and osteogenic differentiation of mesenchymal stem cells (MSCs), as evidenced by the upregulation of ALP, COL1, and Runx2 expression, which were elevated 9.8, 6.5, and >22 times, respectively, compared to pure CS scaffolds. Considering its exceptional in vivo osteogenic potential, we anticipate that the CS/20AMP-GO ink holds great potential for 3D printing of bone grafts.

The use of a nested PCR-RFLP technique, based on the parasite's 18S ribosomal RNA, to characterise Cryptosporidium isolates from HIV/AIDS patients.

Since there have been few studies on human cryptosporidiosis in Iran, attempts were made to identify Cryptosporidium isolates from HIV-positive Iranians, to genotype level. A nested PCR (based on a fragment of the parasite's 18S ribosomal-RNA gene) was first used to see if faecal samples from 35 HIV-positive patients (of whom 17 had apparently been found smear-positive for Cryptosporidium oocysts) contained Cryptosporidium. Twenty-one of the samples (including all 17 of those that appeared smear-positive) were found PCR-positive. Each of these 21 samples was then investigated further, by RFLP analysis in which the amplicons from the secondary PCR were digested with VspI. Curiously, although HIV-infected individuals are known to be susceptible to infection with a wide range of Cryptosporidium genotypes, all the Iranian subjects of the present study were found to be infected with C. hominis (71%) or C. parvum (29%).

The food habit of Epinephelus coioides (Hamilton, 1822) in Khuzestan coastal waters (Persian Gulf).

During January 2000 to March 2001, a large number of Epinephelus coioides species was captured in main fishing areas of Khuzestan province namely, Busafe-Liphe and Bahrakan, North of Persian Gulf. The stomach contents of 394 individuals were analysed, in which 226 individuals had contained (trace-full) stomachs while 168 individuals had empty stomachs. The percentages of CV in males were more than in females. Seventy three percent of stomachs contained fish 11% crab; 8.8% shrimp; 3.9% squid; 1.7% gastropod and 0.4% bivalves. The intensity of feeding in monthly sampling periods and length groups did not show a clear trend. The results of Fp index showed 73% for fish; crab 11%; shrimp 8.8%; squid 3.9%; gastropod 1.7 and 0.4% for bivalvia. Similar pattern of feeding was observed using CN index. The main food items for E. coioides were fish followed by crabs and accidental food items were shrimp, squid, gastropods and bivalvia.