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1.
PLoS One ; 16(5): e0251880, 2021.
Article in English | MEDLINE | ID: mdl-34019573

ABSTRACT

OBJECTIVE: To develop and assess a novel guidance technique and instrumentation system for minimally invasive short-stemmed total shoulder arthroplasty that will help to reduce the complications associated with traditional open replacement such as poor muscle healing and neurovascular injury. We have answered key questions about the developed system including (1) can novel patient-specific guides be accurately registered and used within a minimally invasive environment?; (2) can accuracy similar to traditional techniques be achieved? METHODS: A novel intra-articular patient-specific guide was developed for use with a new minimally invasive posterior surgical approach that guides bone preparation without requiring muscle resection or joint dislocation. Additionally, a novel set of instruments were developed to enable bone preparation within the minimally invasive environment. The full procedure was evaluated in six cadaveric shoulders, using digitizations to assess accuracy of each step. RESULTS: Patient-specific guide registration accuracy in 3D translation was 2.2±1.2mm (RMSE±1 SD; p = 0.007) for the humeral component and 2.7±0.7mm (p<0.001) for the scapula component. Final implantation accuracy was 2.9±3.0mm (p = 0.066) in translation and 5.7-6.8±2.2-4.0° (0.001

Subject(s)
Arthritis/surgery , Arthroplasty, Replacement, Shoulder/methods , Foreign Bodies , Humerus/surgery , Minimally Invasive Surgical Procedures/instrumentation , Scapula/surgery , Shoulder Joint/surgery , Aged , Arthritis/diagnostic imaging , Arthritis/pathology , Arthroplasty, Replacement, Shoulder/instrumentation , Cadaver , Humans , Humerus/diagnostic imaging , Humerus/pathology , Imaging, Three-Dimensional , Precision Medicine , Range of Motion, Articular/physiology , Scapula/diagnostic imaging , Scapula/pathology , Shoulder Joint/diagnostic imaging , Shoulder Joint/pathology , Tomography, X-Ray Computed
2.
Dis Model Mech ; 14(3)2021 03 01.
Article in English | MEDLINE | ID: mdl-33563616

ABSTRACT

Collagen assembly during development is essential for successful matrix mineralisation, which determines bone quality and mechanocompetence. However, the biochemical and structural perturbations that drive pathological skeletal collagen configuration remain unclear. Deletion of vascular endothelial growth factor (VEGF; also known as VEGFA) in bone-forming osteoblasts (OBs) induces sex-specific alterations in extracellular matrix (ECM) conformation and mineralisation coupled to vascular changes, which are augmented in males. Whether this phenotypic dimorphism arises as a result of the divergent control of ECM composition and its subsequent arrangement is unknown and is the focus of this study. Herein, we used murine osteocalcin-specific Vegf knockout (OcnVEGFKO) and performed ex vivo multiscale analysis at the tibiofibular junction of both sexes. Label-free and non-destructive polarisation-resolved second-harmonic generation (p-SHG) microscopy revealed a reduction in collagen fibre number in males following the loss of VEGF, complemented by observable defects in matrix organisation by backscattered electron scanning electron microscopy. This was accompanied by localised divergence in collagen orientation, determined by p-SHG anisotropy measurements, as a result of OcnVEGFKO. Raman spectroscopy confirmed that the effect on collagen was linked to molecular dimorphic VEGF effects on collagen-specific proline and hydroxyproline, and collagen intra-strand stability, in addition to matrix carbonation and mineralisation. Vegf deletion in male and female murine OB cultures in vitro further highlighted divergence in genes regulating local ECM structure, including Adamts2, Spp1, Mmp9 and Lama1. Our results demonstrate the utility of macromolecular imaging and spectroscopic modalities for the detection of collagen arrangement and ECM composition in pathological bone. Linking the sex-specific genetic regulators to matrix signatures could be important for treatment of dimorphic bone disorders that clinically manifest in pathological nano- and macro-level disorganisation. This article has an associated First Person interview with the first author of the paper.


Subject(s)
Extracellular Matrix , Vascular Endothelial Growth Factor A , Animals , Bone and Bones/metabolism , Collagen/metabolism , Extracellular Matrix/metabolism , Female , Humans , Male , Mice , Osteoblasts , Vascular Endothelial Growth Factor A/metabolism
3.
J Shoulder Elbow Surg ; 29(10): 1967-1973, 2020 Oct.
Article in English | MEDLINE | ID: mdl-32499200

ABSTRACT

BACKGROUND: The critical shoulder angle (CSA) has been shown to be correlated with shoulder disease states. The biomechanical hypothesis to explain this correlation is that the CSA changes the shear and compressive forces on the shoulder. The objective of this study is to test this hypothesis by use of a validated computational shoulder model. Specifically, this study assesses the impact on glenohumeral biomechanics of modifying the CSA. METHODS: An inverse dynamics 3-dimensional musculoskeletal model of the shoulder was used to quantify muscle forces and glenohumeral joint forces. The CSA was changed by altering the attachment point of the middle deltoid into a normal CSA (33°), a reduced CSA of 28°, and an increased CSA of 38°. Subject-specific kinematics of slow and fast speed abduction in the scapular plane and slow and fast forward flexion measured by a 3-dimensional motion capture system were used to quantify joint reaction shear and compressive forces. RESULTS: Increasing the CSA results in increased superior-inferior forces (shearing forces; integrated over the range of motion; P < .05). Reducing CSA results in increased lateromedial (compressive) forces for both the maximum and integrated sum of the forces over the whole motion (P < .01). DISCUSSION/CONCLUSION: Changes in the CSA modify glenohumeral joint biomechanics with increasing CSA producing higher shear forces that could contribute to rotator cuff overuse, whereas reducing the CSA results in higher compressive forces that contribute to joint wear.


Subject(s)
Biomechanical Phenomena , Shoulder Joint/physiopathology , Adult , Computer Simulation , Humans , Kinetics , Male , Models, Anatomic , Movement , Range of Motion, Articular , Stress, Mechanical , Young Adult
4.
Matrix Biol Plus ; 5: 100018, 2020 Feb.
Article in English | MEDLINE | ID: mdl-33543015

ABSTRACT

Mineralization of bone is achieved by the sequential maturation of the immature amorphous calcium phase to mature hydroxyapatite (HA) and is central in the process of bone development and repair. To study normal and dysregulated mineralization in vitro, substrates are often coated with poly-l-lysine (PLL) which facilitates cell attachment. This study has used Raman spectroscopy to investigate the effect of PLL coating on osteoblast (OB) matrix composition during differentiation, with a focus on collagen specific proline and hydroxyproline and precursors of HA. Deconvolution analysis of murine derived long bone OB Raman spectra revealed collagen species were 4.01-fold higher in OBs grown on PLL. Further, an increase of 1.91-fold in immature mineral species (amorphous calcium phosphate) was coupled with a 9.32-fold reduction in mature mineral species (carbonated apatite) on PLL versus controls. These unique low mineral signatures identified in OBs were linked with reduced alkaline phosphatase enzymatic activity, reduced Alizarin Red staining and altered osteogenic gene expression. The promotion of immature mineral species and restriction of mature mineral species of OB grown on PLL were linked to increased cell viability and pro-angiogenic vascular endothelial growth factor (VEGF) production. These results demonstrate the utility of Raman spectroscopy to link distinct matrix signatures with OB maturation and VEGF release. Importantly, Raman spectroscopy could provide a label-free approach to clinically assess the angiogenic potential of bone during fracture repair or degenerative bone loss.

5.
Shoulder Elbow ; 11(1 Suppl): 19-25, 2019 May.
Article in English | MEDLINE | ID: mdl-31019558

ABSTRACT

BACKGROUND: Detachment of the central tendon of the supraspinatus from its insertion is considered to be crucial to functional deficit. The aim of the present study was to assess the function of the supraspinatus in terms of abduction moments by introducing different tear configurations to assess the functional effect of the central tendon insertion. METHODS: Ten fresh frozen shoulders from five cadavers were prepared for testing. A testing protocol was established to measure the abduction moment of the supraspinatus under physiological loading tailored to the anthropometrics of each specimen. Four conditions were tested: intact supraspinatus; complete detachment of portion of the supraspinatus tendon anterior to the main central tendon; detachment of the main central tendon; and detachment of the region of the supraspinatus posterior to the main central tendon. RESULTS: There was a significant and large reduction in abduction moment when the central tendon was sectioned (p < 0.05). A smaller reduction in abduction moment was found when the regions anterior and posterior to the main central tendon were sectioned (p < 0.05). CONCLUSIONS: The central tendon is vital in the role of functional arm abduction through force transmission through the intact rotator cuff. Reinsertion of the central tendon in the correct anatomical location is desirable to optimize functional outcome of surgery.

6.
Shoulder Elbow ; 9(1): 15-22, 2017 Jan.
Article in English | MEDLINE | ID: mdl-28572846

ABSTRACT

This paper seeks to outline recent legal developments and requirements pertinent to obtaining informed consent. We argue that this is of particular relevance to patients considering a reverse total shoulder arthroplasty, due to the high complication rate associated with this procedure. By examining the cognitive processes involved in decision-making, and other clinician-related factors such as delivery of information, gender bias and conflict of interest, we explore some of the barriers that can undermine the processes of shared decision-making and obtaining genuine informed consent. We argue that these issues highlight the importance for surgeons in understanding the cognitive processes and other influential factors involved in patients' comprehension and decision-making. We recommend, based on strong evidence, that decision aids could prove useful in overcoming such challenges and could provide one way of mitigating the ethical, professional and legal consequences of failing to obtain proper informed consent. They are not widely used in orthopaedics at present, although it would be in the interests of both the surgeon and patient for such measures to be explored.

7.
J Shoulder Elbow Surg ; 26(9): 1653-1661, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28495573

ABSTRACT

BACKGROUND: Short-stem humeral replacements achieve fixation by anchoring to the metaphyseal trabecular bone. Fixing the implant in high-density bone can provide strong fixation and reduce the risk of loosening. However, there is a lack of data mapping the bone density distribution in the proximal humerus. The aim of the study was to investigate the bone density in proximal humerus. METHODS: Eight computed tomography scans of healthy cadaveric humeri were used to map bone density distribution in the humeral head. The proximal humeral head was divided into 12 slices parallel to the humeral anatomic neck. Each slice was then divided into 4 concentric circles. The slices below the anatomic neck, where short-stem implants have their fixation features, were further divided into radial sectors. The average bone density for each of these regions was calculated, and regions of interest were compared using a repeated-measures analysis of variance with significance set at P < .05. RESULTS: Average apparent bone density was found to decrease from proximal to distal regions, with the majority of higher bone density proximal to the anatomic neck of the humerus (P < .05). Below the anatomic neck, bone density increases from central to peripheral regions, where cortical bone eventually occupies the space (P < .05). In distal slices below the anatomic neck, a higher bone density distribution in the medial calcar region was also observed. CONCLUSION: This study indicates that it is advantageous with respect to implant fixation to preserve some bone above the anatomic neck and epiphyseal plate and to use the denser bone at the periphery.


Subject(s)
Arthroplasty, Replacement, Shoulder , Humeral Head/diagnostic imaging , Prosthesis Design , Shoulder Prosthesis , Aged , Bone Density , Cadaver , Female , Humans , Male , Middle Aged , Tomography, X-Ray Computed
8.
J Shoulder Elbow Surg ; 26(3): 512-520, 2017 Mar.
Article in English | MEDLINE | ID: mdl-27745804

ABSTRACT

BACKGROUND: The deltopectoral approach for total shoulder arthroplasty can result in subscapularis dysfunction. In addition, glenoid wear is more prevalent posteriorly, a region difficult to access with this approach. We propose a posterior approach for access in total shoulder arthroplasty that uses the internervous interval between the infraspinatus and teres minor. This study compares this internervous posterior approach with other rotator cuff-sparing techniques, namely, the subscapularis-splitting and rotator interval approaches. METHODS: The 3 approaches were performed on 12 fresh frozen cadavers. The degree of circumferential access to the glenohumeral joint, the force exerted on the rotator cuff, the proximity of neurovascular structures, and the depth of the incisions were measured, and digital photographs of the approaches in different arm positions were analyzed. RESULTS: The posterior approach permits direct linear access to 60% of the humeral and 59% of the glenoid joint circumference compared with 39% and 42% for the subscapularis-splitting approach and 37% and 28% for the rotator interval approach. The mean force of retraction on the rotator cuff was 2.76 (standard deviation [SD], 1.10) N with the posterior approach, 2.72 (SD, 1.22) N with the rotator interval, and 4.75 (SD, 2.56) N with the subscapularis-splitting approach. From the digital photographs and depth measurements, the estimated volumetric access available for instrumentation during surgery was comparable for the 3 approaches. CONCLUSION: The internervous posterior approach provides greater access to the shoulder joint while minimizing damage to the rotator cuff.


Subject(s)
Arthroplasty, Replacement, Shoulder/methods , Shoulder Joint/surgery , Aged , Aged, 80 and over , Cadaver , Feasibility Studies , Female , Humans , Image Processing, Computer-Assisted , Male , Middle Aged , Minimally Invasive Surgical Procedures/methods , Photography , Rotator Cuff
10.
J Bone Joint Surg Am ; 96(13): e112, 2014 Jul 02.
Article in English | MEDLINE | ID: mdl-24990986

ABSTRACT

BACKGROUND: Surgical training is undergoing major changes with reduced resident work hours and an increasing focus on patient safety and surgical aptitude. The aim of this study was to create a valid, reliable method for an assessment of arthroscopic skills that is independent of time and place and is designed for both real and simulated settings. The validity of the scale was tested using a virtual reality shoulder arthroscopy simulator. METHODS: The study consisted of two parts. In the first part, an Imperial Global Arthroscopy Rating Scale for assessing technical performance was developed using a Delphi method. Application of this scale required installing a dual-camera system to synchronously record the simulator screen and body movements of trainees to allow an assessment that is independent of time and place. The scale includes aspects such as efficient portal positioning, angles of instrument insertion, proficiency in handling the arthroscope and adequately manipulating the camera, and triangulation skills. In the second part of the study, a validation study was conducted. Two experienced arthroscopic surgeons, blinded to the identities and experience of the participants, each assessed forty-nine subjects performing three different tests using the Imperial Global Arthroscopy Rating Scale. Results were analyzed using two-way analysis of variance with measures of absolute agreement. The intraclass correlation coefficient was calculated for each test to assess inter-rater reliability. RESULTS: The scale demonstrated high internal consistency (Cronbach alpha, 0.918). The intraclass correlation coefficient demonstrated high agreement between the assessors: 0.91 (p < 0.001). Construct validity was evaluated using Kruskal-Wallis one-way analysis of variance (chi-square test, 29.826; p < 0.001), demonstrating that the Imperial Global Arthroscopy Rating Scale distinguishes significantly between subjects with different levels of experience utilizing a virtual reality simulator. CONCLUSIONS: The Imperial Global Arthroscopy Rating Scale has a high internal consistency and excellent inter-rater reliability and offers an approach for assessing technical performance in basic arthroscopy on a virtual reality simulator. CLINICAL RELEVANCE: The Imperial Global Arthroscopy Rating Scale provides detailed information on surgical skills. Although it requires further validation in the operating room, this scale, which is independent of time and place, offers a robust and reliable method for assessing arthroscopic technical skills.


Subject(s)
Arthroscopy/education , Clinical Competence , Educational Measurement/methods , Shoulder Joint/surgery , Delphi Technique , Humans , Psychomotor Performance , Reproducibility of Results , Video Recording
11.
Article in English | MEDLINE | ID: mdl-23057478

ABSTRACT

An accurate diagnosis of glenohumeral joint (GHJ) instability is essential for an effective surgical intervention. There is presently no known comprehensive algorithm of clinical tests for the confirmation of the functional integrity of glenohumeral ligaments (GHLs). A validated computational GHL strain analyser was applied to a set of GHJ kinematics data from the literature to simulate 57 different physiological clinical examination manoeuvres. An algorithm that integrates the GHL pre-straining activities at the toe region of the stress-strain curve was developed for the quantification of ligament loading from prevailing strains. This was used to upgrade the strain analyser and applied to produce a matrix of the various GHL loadings and sensitivities during the manoeuvres. The investigation magnified the likely impact of anatomical variations of GHL attachments as possible causes of misdiagnoses during clinical examinations of GHJ dysfunction. This can serve as an assistive guide to ascertain the functional condition of a specific GHL during symptomatic clinical examinations.


Subject(s)
Joint Instability/diagnosis , Ligaments, Articular/physiopathology , Shoulder Joint , Algorithms , Biomechanical Phenomena , Computer Simulation , Humans , Sensitivity and Specificity
12.
Proc Inst Mech Eng H ; 226(6): 461-8, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22783762

ABSTRACT

Analysis of the function of glenohumeral ligaments (GHLs) during physical joint manipulations is hindered by an inability to adequately image these tissues during the movements. This restricts functional biomechanics studies only to the manoeuvres that may be replicated cadaverically. There is, however, a clinical imperative to be able to investigate complex manoeuvres that exacerbate symptoms but cannot be easily conducted physically in the laboratory. The aim of this study was to develop and validate an algorithm for a computer simulation model that allows the quantification of glenohumeral ligament lengths during function. Datasets of the humerus and scapula pair were segmented to provide individual surface meshes of the bones and insertion points of each glenohumeral ligament on both bones. An algorithm was developed in which the glenohumeral ligament attachment-to-attachment length was divided into two straight lines, plus an arc overlaying the spherical wrapping portions. The model was validated by simulating two classical cadaveric studies from the literature and comparing results. Predictions from the model were qualitatively similar to the results of the two cadaveric studies by a factor of 91.7% and 81.8%, respectively. Algorithm application will allow investigation of functional loading of the glenohumeral ligaments during simulated complex motions. This could then be used to provide diagnostic understanding and thus, inform surgical reconstruction.


Subject(s)
Algorithms , Computer Simulation , Ligaments, Articular/physiology , Models, Anatomic , Shoulder Joint/physiology , Biomechanical Phenomena , Cadaver , Humans , Humerus/physiology , Scapula/physiology
13.
J Anat ; 220(5): 525-8, 2012 May.
Article in English | MEDLINE | ID: mdl-22404237

ABSTRACT

Functional biomechanics studies of the glenohumeral (GH) soft tissues require an understanding of their sites of bony attachment. Anatomical positions of GH capsular structures have often been quantified relative to the rims of the glenoid and humeral head (HH). The aim of this study was twofold: (1) to quantify the reliability of a set of protractors that directly fit on to the glenoid and HH rims and (2) to use this to determine direct angular position referencing of landmarks and soft tissue attachment points. Three assessors independently used the protractors to assess nine prescribed landmarks on 30 dry bone specimens (15 glenoids and 15 HHs) recording the angular positions of the structures relative to the glenoid and HH. The collected data showed high levels of validity as indicated by the protractor's intra- and inter-assessor reliabilities: 98.2 and 98.7% for the glenoid component, and 96.2 and 96.5% for the humeral component, respectively. The device could be useful in anatomical studies, description of defects and pathologies on glenohumeral articulation, and planning of scapular reconstructive surgery.


Subject(s)
Glenoid Cavity/anatomy & histology , Humeral Head/anatomy & histology , Mathematics/instrumentation , Anatomy/instrumentation , Humans , Observer Variation , Reproducibility of Results , Shoulder Joint/anatomy & histology
14.
J Shoulder Elbow Surg ; 21(6): 749-53, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22078083

ABSTRACT

BACKGROUND: Tears of the supraspinatus are common and incompletely understood. The degree of fatty infiltration into the muscle is perceived to be a determining factor of successful surgical repair and postoperative function. It is the hypothesis of this study that the degree of central tendon retraction (CTR) as seen on magnetic resonance imaging corresponds to the amount of fatty infiltration classified according to the Goutallier grading system. MATERIALS AND METHODS: Magnetic resonance imaging scans of the supraspinatus were reviewed in 2 identifiable groups: 143 scans with no tear (NT) and 148 scans with a full-thickness tear (FTT) and CTR. The degree of CTR and the corresponding Goutallier grade were measured. The difference in Goutallier grade between the NT and FTT groups was measured with the Mann-Whitney test. The relationship between Goutallier grade and increasing amount of CTR was described by use of Spearman rank correlation. Studying the difference between the Goutallier grades and CTR was achieved by use of Mann-Whitney tests. RESULTS: Of NT scans, 100% showed Goutallier grade 0 or 1. Among FTT scans, 2 showed grade 0, 21 showed grade 1, 35 showed grade 2, 14 showed grade 3, and 76 showed grade 4. The difference was statistically significant (P < .001) between the Goutallier grade 3 and 4 scans, and the degree of Goutallier grade increased with increasing CTR (P < .001). CONCLUSION: Fatty infiltration can be directly linked to CTR and, as such, may help to determine surgical intervention between groups.


Subject(s)
Adipose Tissue/pathology , Rotator Cuff Injuries , Tendons/pathology , Cross-Sectional Studies , Humans , Magnetic Resonance Imaging , Rotator Cuff/pathology , Rotator Cuff/surgery , Rupture , Tendons/surgery
15.
J Shoulder Elbow Surg ; 20(8): 1194-9, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21778073

ABSTRACT

BACKGROUND: The prevalence of failure among repairs of the rotator cuff is well known, but very few objective data exist regarding either the scale or timing of this complication. The aim of this study was to use a previously validated modified technique of roentgen stereophotogrammetric analysis to monitor the behavior of the rotator cuff after repair to establish at what point failure may occur. MATERIALS AND METHODS: A series of 10 patients had metal beads and wire sutures embedded into the humeral greater tuberosity and supraspinatus tendon, respectively, during open cuff repair procedures. Roentgen stereophotogrammetric analysis imaging of the repaired rotator cuffs was performed at set intervals during the first year after surgery. RESULTS: The mean distance between the tendon and bone markers did not increase significantly between the time of surgery and 3 to 4 weeks. There was then a significant increase of 7.0 mm in the distance between the tendon and bone markers, with the largest increase occurring between 3 to 4 weeks and 12 to 14 weeks after surgery, as well as a further small but significant increase of 1.7 mm between 12 to 14 weeks and 1 year. These results were compared with clinical examination and ultrasound findings. CONCLUSION: Most tendon marker movement was seen during the most intensive period of physiotherapy, in the second and third months after surgery. Significantly more movement was seen in the tendon markers of those patients in whom the repair failed.


Subject(s)
Arthroscopy/methods , Radiostereometric Analysis/methods , Rotator Cuff/surgery , Shoulder Injuries , Suture Techniques/instrumentation , Sutures , Tendons/surgery , Adult , Aged , Female , Follow-Up Studies , Humans , Male , Middle Aged , Prospective Studies , Rotator Cuff Injuries , Shoulder Joint/surgery , Treatment Failure
16.
J Shoulder Elbow Surg ; 20(6): 899-903, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21454103

ABSTRACT

HYPOTHESIS: It is hypothesized that supraspinatus central tendon retraction is related to functional deficit; yet, there is no literature comparing the normal and pathological pennation and central tendon angles using magnetic resonance imaging (MRI). Therefore, the aim of this study was to quantify the anterior and posterior muscle pennation angles, central tendon angle, and retraction of the supraspinatus using MRI. METHODS: Anterior pennation angle (APA), posterior pennation angle (PPA), and central tendon angle (CTA) were measured from 2 groups: no tear (NT, n = 157), full thickness tears (FTT) with retraction (FTT, n = 156). RESULTS: No tear (NT) average APA, PPA, and CTA were 19.0° (SD 5.9), 4.0° (SD 3.2), and 17.8° (SD 5.1). All differences were statistically significant (PPA < APA, P < .001; PPA < CTA, P < .001; CTA < APA, P < .001). FTT averages were 17.6° (SD 8.6), 16.7° (SD 12.2), and 7.3° (SD 4.9). Increasing retraction correlated to PPA (P < .001), APA (P < .002), and CTA (P < .001). CONCLUSION: The size of a supraspinatus tear is directly correlated with muscle pennation and tendon retraction, and provides a direct measurement from MRI that can now be employed in further studies of functional deficit and tendon tear size.


Subject(s)
Magnetic Resonance Imaging , Shoulder , Tendon Injuries/pathology , Tendons/anatomy & histology , Female , Humans , Male , Middle Aged
17.
J Shoulder Elbow Surg ; 20(7): 1125-32, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21481611

ABSTRACT

HYPOTHESIS: This study evaluated several classification systems and expert surgeons' anatomic understanding of these complex injuries based on a consecutive series of patients. We hypothesized that current proximal humeral fracture classification systems, regardless of imaging methods, are not sufficiently reliable to aid clinical management of these injuries. MATERIALS AND METHODS: Complex fractures in 96 consecutive patients were investigated by generation of rapid sequence prototyping models from computed tomography Digital Imaging and Communications in Medicine (DICOM) imaging data. Four independent senior observers were asked to classify each model using 4 classification systems: Neer, AO, Codman-Hertel, and a prototype classification system by Resch. Interobserver and intraobserver κ coefficient values were calculated for the overall classification system and for selected classification items. RESULTS: The κ coefficient values for the interobserver reliability were 0.33 for Neer, 0.11 for AO, 0.44 for Codman-Hertel, and 0.15 for Resch. Interobserver reliability κ coefficient values were 0.32 for the number of fragments and 0.30 for the anatomic segment involved using the Neer system, 0.30 for the AO type (A, B, C), and 0.53, 0.48, and 0.08 for the Resch impaction/distraction, varus/valgus and flexion/extension subgroups, respectively. Three-part fractures showed low reliability for the Neer and AO systems. DISCUSSION: Currently available evidence suggests fracture classifications in use have poor intra- and inter-observer reliability despite the modality of imaging used thus making treating these injuries difficult as weak as affecting scientific research as well. This study was undertaken to evaluate the reliability of several systems using rapid sequence prototype models. CONCLUSION: Overall interobserver κ values represented slight to moderate agreement. The most reliable interobserver scores were found with the Codman-Hertel classification, followed by elements of Resch's trial system. The AO system had the lowest values. The higher interobserver reliability values for the Codman-Hertel system showed that is the only comprehensive fracture description studied, whereas the novel classification by Resch showed clear definition in respect to varus/valgus and impaction/distraction angulation.


Subject(s)
Shoulder Fractures/classification , Shoulder Fractures/diagnostic imaging , Humans , Image Processing, Computer-Assisted , Observer Variation , Reproducibility of Results , Tomography, X-Ray Computed
18.
J Shoulder Elbow Surg ; 17(5): 784-9, 2008.
Article in English | MEDLINE | ID: mdl-18504147

ABSTRACT

The purpose of this study was to examine the tendon-to-bone interface pressure, contact area, and force after reattaching a tendon to bone by use of a suture and suture anchor. Repairs were made in 8 ovine shoulders in vitro, by use of 3 suture types in each: Ethibond, polydioxanone, or Orthocord. A Tekscan pressure sensor was placed between the tendon and bone and monitored for 1 hour after the repair. The principal finding was a significant loss of approximately 60% of the contact parameters immediately after the suture was tied, followed by further significant loss over the next hour to a mean of only 14% of the initial readings. We concluded that pressure measurement systems that only record the initial maximum pressure would yield overly optimistic results for the actual repair pressure after the repair is completed. The Tekscan system, however, allowed us to monitor pressure reductions that occurred both during and after the repair.


Subject(s)
Humerus/surgery , Orthopedic Procedures , Rotator Cuff/physiopathology , Rotator Cuff/surgery , Suture Anchors , Animals , Biomechanical Phenomena , Humerus/physiopathology , Manometry , Pressure , Rotator Cuff Injuries , Sheep , Shoulder , Suture Techniques , Sutures , Time Factors
20.
J Cell Physiol ; 214(2): 537-44, 2008 Feb.
Article in English | MEDLINE | ID: mdl-17685428

ABSTRACT

Communication between endothelial and bone cells is crucial for controlling vascular supply during bone growth, remodeling, and repair but the molecular mechanisms coordinating this intercellular crosstalk remain ill-defined. We have used primary human and rat long bone-derived osteoblast-like cells (HOB and LOB) and human umbilical vein endothelial cells (HUVEC) to interrogate the potential autocrine/paracrine role of vascular endothelial cell growth factor (VEGF) in osteoblast:endothelial cell (OB:EC) communication and examined whether prostaglandins (PG), known modulators of both OB and EC behavior, modify VEGF production. We found that the stable metabolite of PGI2, 6-keto-PGF(1alpha) and PGE2, induced a concentration-dependent increase in VEGF release by HOBs but not ECs. In ECs, VEGF promoted early ERK1/2 activation, late cyclooxygenase-2 (COX-2) protein induction, and release of 6-keto-PGF1alpha. In marked contrast, no significant modulation of these events was observed in HOBs exposed to VEGF, but LOBs clearly exhibited COX-dependent prostanoid release (10-fold less than EC) following VEGF treatment. A low level of osteoblast-like cell responsiveness to exogenous VEGF was supported by VEGFR2/Flk-1 immunolabelling and by blockade of VEGF-mediated prostanoid generation by a VEGFR tyrosine kinase inhibitor (TKI). HOB alkaline phosphatase (ALP) activity was increased following long-term non-contact co-culture with ECs and exposure of ECs to VEGF in this system further increased OB-like cell differentiation and markedly enhanced prostanoid release. Our studies confirm a paracrine EC-mediated effect of VEGF on OB-like cell behavior and are the first supporting a model in which prostanoids may facilitate this unidirectional VEGF-driven OB:EC communication. These findings may offer novel regimes for modulating pathological bone remodeling anomalies through the control of the closely coupled vascular supply.


Subject(s)
Endothelial Cells/metabolism , Osteoblasts/metabolism , Paracrine Communication , Signal Transduction , Vascular Endothelial Growth Factor A/metabolism , Acromion/cytology , Acromion/surgery , Alkaline Phosphatase/analysis , Animals , Cell Differentiation , Cells, Cultured , Coculture Techniques , Collagen Type I/analysis , Culture Media/chemistry , Culture Media/pharmacology , Dinoprostone/analysis , Dinoprostone/metabolism , Endothelial Cells/cytology , Endothelium, Vascular/cytology , Epidermal Growth Factor/metabolism , Epoprostenol/analysis , Epoprostenol/metabolism , Female , Femur/cytology , Fluorescein-5-isothiocyanate , Fluorescent Antibody Technique, Direct , Fluorescent Dyes , Humans , Interleukin-1alpha , Organ Culture Techniques , Osteoblasts/cytology , Osteoblasts/drug effects , Osteocalcin/analysis , Propidium , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Time Factors , Umbilical Veins/cytology , Vascular Endothelial Growth Factor A/analysis , Vascular Endothelial Growth Factor A/genetics
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