ABSTRACT
Prostaglandin (PG) F2 alpha and PGE were measured in 163 semen samples from 145 men attending our male infertility clinic. In addition, each semen sample was analyzed for 13 different fertility parameters. Blood plasma levels of testosterone, follicle-stimulating hormone, and luteinizing hormone were also determined in many of the patients. The data obtained were then analyzed using multiple regression analyses on each PG for all of the parameters studied. The results indicate seminal PGF2 alpha and PGE concentrations were 2.78 +/- 0.24 micrograms/ml and 46.0 +/- 4.5 micrograms/ml, respectively. The seminal parameters that were significant predictors of seminal PGF2 alpha included: Ca++ concentration (P less than 0.001), Zn++ concentration (P less than 0.01), and percentage of tapered sperm (P less than 0.05). The seminal parameters that were significant predictors of seminal PGE included: Ca++ concentration (P less than 0.01) and sperm motility (P less than 0.05). Plasma testosterone was also a significant predictor of seminal PGE (P less than 0.05). These results suggest that seminal PGs are important to the human male fertility potential in that their levels are significantly interdependent with specific parameters of male fertility.
Subject(s)
Infertility, Male/metabolism , Prostaglandins/analysis , Semen/analysis , Adult , Dinoprost , Follicle Stimulating Hormone/blood , Humans , Luteinizing Hormone/blood , Male , Middle Aged , Prostaglandins E/analysis , Prostaglandins F/analysis , Testosterone/bloodSubject(s)
Fertility , Prostate/metabolism , Prostatitis/physiopathology , Humans , Hydrogen-Ion Concentration , Leukocyte Count , Male , Neutrophils , Phagocytes , Semen , Sperm Motility , Zinc/metabolismABSTRACT
Determinations of sperm morphology and viability are useful techniques for assessment of semen quality. In this report eosin-nigrosin is compared with hematoxylin-eosin for effectiveness in characterizing spermatozoa. The results indicate that eosin-nigrosin and hematoxylineosin give comparable results in differentiating spermatozoa. In addition, eosin-nigrosin allows the determination of the per cent of viable cells in each morphologic category. The eosin-nigrosin staining technique is a simple, rapid method which may give additional information in the assessment of male infertility.
Subject(s)
Fertility , Semen/cytology , Spermatozoa/cytology , Evaluation Studies as Topic , Histological Techniques , Humans , Male , Staining and Labeling/methodsABSTRACT
Two commonly used live-dead stains (eosin-nigrosin (EN) and eosin-opal blue (EOB)) were compared with the estimated active spermatozoa in semen samples from patients attending an infertility service. Twenty-eight semen samples were analyzed throughout the day of their collection by estimating the number of active spermatozoa and by staining a portion of the incubated sample (37 degrees C) with each stain. The samples were analyzed 30, 60, 120, 240, and 360 minutes after the initial collection. At 30 minutes there were no significant differences between the estimated values and those of either stain. The slope of the EN stain closely paralleled that of the estimated measurements throughout the remainder of the time periods, while the EOB slope was somewhat steeper. Repeatability of values for semen samples obtained on different days was generally good for each stain. A comparison of semen samples from 85 patients, 30 minutes after collection, showed no significant differences between the numbers of estimated active sperm and the percentage live using the EN stain. Studies of 132 semen samples using the EN stain showed a positive correlation with the over-all quality of sperm motility. The results indicate that there is a role for live-dead staining in assessing semen quality.
