Designing of ZnO nanoparticles oriented interface imprinted electrochemical sensor for fluoxetine detection.

This study represents nanoparticle-based well-oriented recognition sites via interface imprinting, followed by selective and sensitive determination of fluoxetine (FLX). Herein, FLX was firstly immobilized onto ZnO NPs, and then polymerization was carried out with MAPA, HEMA, and EGDMA on the glassy carbon electrode via photopolymerization. After the etching of ZnO with and 10 mM HCI solution, a porous structure with recognition sites for FLX was constructed onto surface. The characterization of the electrochemical sensor was accomplished by utilizing CV, EIS, ATR-FTIR AFM, and SEM analysis. The DPV was used to determine FLX in standard solution, serum sample, and tap water. The effect of FLX concentration variation was studied using the DPV in the range of 1.0 × 10-11 M to 1.0 × 10-10 M with a detection limit of 2.67 × 10-12 M. This sensor showed specific recognition toward template, and more than 90% of its original response was retained after being stored in the desiccator at R.T. for 5 days. This technique has proven to be a powerful, highly selective, and sensitive tool for the rapid detection of FLX in tap water and spike serum samples.

Hydrophobic cryogels for DNA adsorption: effect of embedding of monosize microbeads into cryogel network on their adsorptive performances.

As alternative hydrophobic adsorbent for DNA adsorption, supermacroporous cryogel disks were synthesized via free radical polymerization. In this study, we have prepared two kinds of cryogel disks: (i) poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan) [p(HEMA-MATrp)] cryogel containing specific hydrophobic ligand MATrp; and (ii) monosize p(HEMA-MATrp) particles synthesized via suspension polymerization embedded into p(HEMA) cryogel structure to obtain p(HEMA-MATrp)/p(HEMA) composite cryogel disks. These cryogel disks containing hydrophobic functional group were characterized via swelling studies, Fourier transform infrared spectroscopy, elemental analysis, surface area measurements and scanning electron microscopy. DNA adsorption onto both p(HEMA-MATrp) cryogel and p(HEMA-MATrp)/p(HEMA) composite cryogels was investigated. Maximum adsorption of DNA on p(HEMA-MATrp) cryogel was found to be 15 mg/g polymer. Otherwise, p(HEMA-MATrp)/p(HEMA) composite cryogels significantly increased the DNA adsorption capacity to 38 mg/g polymer. Composite cryogels could be used repeatedly without significant loss on adsorption capacity after 10 repetitive adsorption-desorption cycles.