Positive rates of anti-acari-borne disease antibodies of rural inhabitants in Japan.

An assessment of acari (tick and mite) borne diseases was required to support development of risk management strategies in rural areas. To achieve this objective, blood samples were mainly collected from rural residents participating in hunting events. Out of 1,152 blood samples, 93 were positive against acari-borne pathogens from 12 prefectures in Japan. Urban areas had a lower rate of positive antibodies, whereas mountainous farming areas had a higher positive antibody prevalence. Residents of mountain areas were bitten by ticks or mites significantly more often than urban residents. Resident of mountain areas, including hunters, may necessary to be educated for prevention of akari-borne infectious diseases.

TERATOMA OF THE OVARY IN A FREE-RANGING JAPANESE RACCOON DOG (NYCTEREUTES PROCYONOIDES VIVERRINUS).

A young adult, female, free-ranging Japanese raccoon dog ( Nyctereutes procyonoides viverrinus) with scabies infection was found dead as a result of traumatic injuries presumed to reflect vehicular trauma. Necropsy showed a large solid mass located on the left ovarian region, occupying a third of the abdominal cavity. Histologically, the mass contained complex tissues derived from three germinal layers, with areas of cuboidal or columnar epithelium, keratinized squamous epithelium, bone, cartilage, and adipose tissue. This paper presents the first morphologic description of ovarian teratoma in a raccoon dog.

Genotypes and haplotypes of CCR2 and CCR3 genes in Japanese cedar pollinosis.

Whole genome scan analyses have revealed that the chromosomal region 3p21.3, which contains a gene cluster of the CC chemokine receptor, is possibly critical for the pathogenesis of allergic inflammation. Japanese cedar pollinosis is mediated by a type I allergy and induces seasonal rhinitis and conjunctivitis in humans as the most common form of hay fever in spring in Japan, although the candidate genes for cedar pollinosis remain to be elucidated. We sequenced CCR1, CCR2, CCR3, CCR5, and CCXCR1 using the PCR restriction fragment length polymorphism method in subjects with cedar pollinosis and controls. We found 8 polymorphisms of A111G, Arg127Cys and Arg252Gln in CCXCR1, T885C in CCR1, Val64Ile and T780C in CCR2, T51C in CCR3 and Arg223Gln in CCR5. The transmission disequilibrium test using 60 children with pollinosis and their parents and an association study using unrelated adult subjects (151 patients and 157 controls) showed a significant association of 64Ile in CCR2 and 51C in CCR3 with cedar pollinosis. The frequency of haplotype 64Ile/780C/51C in pollinosis was significantly higher than in controls. Our results suggest that CCR2 and CCR3 genes are candidate genes for Japanese cedar pollinosis.

[Usefulness and practical use of an automatic counter for scattering pollen in spring].

BACKGROUND: In this decade some automatic pollen counters have been developed and showed them useful in some reports. However, the results of automatic counting have not been established. METHOD: We have compared scattering cedar and cypress pollen counts with Durham pollen samplers and a newly developed automatic pollen counter (KH-3000) in the spring from 2000 to 2004 in Tokyo. RESULTS: The relationship between the data of the automatic counter and that of a Durham sampler method is so good that this automatic counter seems useful. The best relationship between two methods was seen from late February to early April in the season of high amounts of pollens scatters. The ratio of pollen counts measured with automatic counter and Durham sampler was about 3.5 on average in the period of enough cedar and cypress pollen dispersion. The avoidance of pollens seems possible with real-time pollen information because reliability of automatic counting is highly evaluated when enough pollens scatter. CONCLUSION: In future, the standardization of automatic counting should be necessary for the accurate evaluation of scattering pollens.

Increasing prevalence of Japanese cedar pollinosis: a meta-regression analysis.

BACKGROUND: Japanese cedar pollinosis, caused by the pollen of the Japanese cedar tree (Cryptomeria japonica), is the commonest seasonal allergic disease in Japan. A number of epidemiological surveys have been reported on Japanese cedar pollinosis, but it has never been assessed systematically or quantitatively. To confirm the increasing prevalence of Japanese cedar pollinosis and related factors, we conducted a meta-regression analysis on population-based surveys in Japan. METHODS: We searched for data from population-based surveys in which serological methods were used to test all participants. Weighted regression of logit-transformed prevalence and sensitization rates were used to evaluate the effects of the year of survey, age, and degree of urbanization. We also analyzed the relationship between prevalence and sensitization rate. RESULTS: Thirty-eight reports with 27 subgroups for prevalence and 134 subgroups for sensitization rate were selected from the literature published in the years between 1986 and 2000. The Japanese cedar pollen sensitization rate was found to be significantly correlated with the year of survey, age, and degree of urbanization (adjusted R(2) = 0.55). The coefficient for the correlation between the prevalence and the sensitization rate revealed a statistically significant correlation (Pearson's r = 0.70, p < 0.001). CONCLUSIONS: The prevalence of Japanese cedar pollinosis among adolescents was predicted to be 28.7% in metropolitan areas and 24.5% in the general population in urban areas in the year 2004, derived from the estimated sensitization rate and the relationship between sensitization rate and prevalence. The prevalence of Japanese cedar pollinosis increased 2.6-fold between 1980 and 2000, and the prevalence differed considerably according to age and degree of urbanization.

Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis.

BACKGROUND: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. METHODS: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. RESULTS: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. CONCLUSIONS: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

High contribution contrast between the genes of eosinophil peroxidase and IL-4 receptor alpha-chain in Japanese cedar pollinosis.

BACKGROUND: Japanese cedar pollinosis is the most common form of hayfever in Japan in spring and has remarkably increased since 1960. OBJECTIVE: We sought to clarify the candidate genes for cedar pollinosis using a case-control study. METHODS: After diagnosing 351 subjects on the basis of an intradermal test, nasal provocation test, and questionnaire regarding nasal and conjunctival symptoms, we determined the blood-specific IgE values and genotypes of eosinophil peroxidase (EPO) and interleukin-4 receptor alpha-chain (IL4RA) in 145 patients with pollinosis and 206 healthy subjects, including 75 healthy subjects with higher specific IgE values. RESULTS: We found significant differences in the frequencies of Pro358Leu in EPO and of Ile50Val and Glu375Ala in IL4RA between patients and healthy subjects. There was a significantly higher frequency of 358Leu in EPO in patients than in healthy subjects showing a higher specific IgE value. In contrast, we recognized significant changes in the prevalence of Ile50Val and Glu375Ala in IL4RA in healthy subjects with a normal IgE value compared with those in healthy subjects with a higher specific IgE value. The relationship between EPO polymorphisms and the onset of symptoms was exactly opposite that for IL4RA. CONCLUSIONS: These results suggest that Pro358Leu in EPO is strongly involved in the development of cedar pollinosis. Ile50Val and Glu375Ala in IL4RA seem to be related to cedar pollen sensitization. Subjects with Ile50 or Glu375 might develop cedar pollinosis with increased exposure to cedar pollen.

[Effects of quantity of Japanese cedar pollen, air pollution and urbanization on allergic rhinitis morbidity in Ibaraki prefecture].

OBJECTIVES: It has been reported that morbidity from allergic rhinitis in the National Health Insurance records in Ibaraki Prefecture for May correlated with the quantity of Japan cedar pollen scattered in each year. The purpose of the present investigation was to clarify the Japanese cedar pollinosis contribution to morbidity, and also clarifying the influence of air pollution and medical resources on the crisis and symptoms of allergic rhinitis. METHODS: The charts in four otolaryngology facilities were used for analyzing the Japan cedar pollinosis content with reference to the allergic rhinitis during the pollen season. The age-adjusted morbidity of allergic rhinitis was annually compared employing data of National Health Insurance records for medical examinations made in May during the period between 1988 and 1996 in Ibaraki Prefecture. The quantity of Japanese cedar pollen was measured at seven area points in Ibaraki Prefecture during the three-year period from 1994 to 1996, and was compared with the degree of Japan cedar wood occupation in each municipality. Traffic volume according to municipalities in Ibaraki Prefecture was taken as a surrogate indicator of air pollution. The area otolaryngology facilities and doctors were taken as medical resources. Values were thus compared with allergic rhinitis morbidity. RESULTS: Sixty to eighty percent of the allergic rhinitis patients examined in May were found to be suffering from pollinosis. The quantities of Japanese cedar pollen scatter at the seven points in Ibaraki Prefecture varied in concert every year, the quantities correlating well with the area of Japanese cedar woods stands in each municipality in some but not in other years. The morbidity in the records of allergic rhinitis according to municipalities correlated negatively with the proportion of the population occupied in farming (r = -0.38) and with the area of Japanese cedar woods in each municipality (r = -0.40). The traffic volume calculated according to municipalities in Ibaraki Prefecture correlated significantly with the yearly average concentration of nitrogen dioxide (r = 0.63, P < 0.01) and with the morbidity of allergic rhinitis. In places among the northern mountains of the Prefecture, both the morbidity in the records and the distribution of otolaryngology facilities were low. CONCLUSIONS: More than 60% of the morbidity from allergic rhinitis in May appeared in the National Health Insurance records to be associated with Japanese cedar pollinosis. This was confirmed by the correlation between yearly variation in morbidity and that of the quantity of Japanese cedar pollen scatter. As local factors increasing the morbidity of allergic rhinitis in the records, air pollution, urbanization and a greater availability of medical resources were indicated.