1.
Anal Biochem
; 91(1): 138-45, 1978 Nov.
Article
in English
| MEDLINE
| ID: mdl-9762092
ABSTRACT
The aglycone, 3-hydroxybenzo[a]pyrene, was metabolized to 3-benzo[a]pyrenyl-beta-D-glucopyranosiduronic acid in the presence of uridine 5'-diphosphoglucuronic acid and rabbit liver microsomes. The course of the biosynthetic reaction was followed by fluorimetry and reverse-phase, paired-ion high pressure liquid chromatography (HPLC). Also, the HPLC system was used to analyze for glucuronide and 3-hydroxybenzo[a]pyrene during the isolation procedure. The existence of a glucuronide of 3-hydroxybenzo[a]pyrene was determined by radiotracer and enzymic techniques, utilizing the HPLC system. Field desorption and direct inlet mass spectral techniques were used to characterize the 3-hydroxybenzo[a]pyrene glucuronide.
Subject(s)
Benzopyrenes/isolation & purification , Carcinogens/isolation & purification , Animals , Benzopyrenes/analysis , Benzopyrenes/metabolism , Carcinogens/analysis , Carcinogens/metabolism , Chromatography, High Pressure Liquid/methods , In Vitro Techniques , Mass Spectrometry , Microsomes, Liver/metabolism , Rabbits , Spectrometry, Fluorescence
2.
Anal Biochem
; 78(2): 520-6, 1977 Apr.
Article
in English
| MEDLINE
| ID: mdl-851224
3.
Biochem Pharmacol
; 25(2): 227-30, 1976 Jan 15.
Article
in English
| MEDLINE
| ID: mdl-1259790
4.
J Chromatogr
; 29(1): 232-4, 1967 Jul.
Article
in English
| MEDLINE
| ID: mdl-6054410
5.
J Am Pharm Assoc
; 6(9): 460-2, 1966 Sep.
Article
in English
| MEDLINE
| ID: mdl-5943501
6.
J Pharm Sci
; 55(1): 115-6, 1966 Jan.
Article
in English
| MEDLINE
| ID: mdl-5918639