ABSTRACT
A procedure is described for the detection of azaperone, propiopromazine and carazolol in pig muscle, liver and kidney tissue. The method comprises extraction from an alkaline tissue homogenate with diethyl ether, followed by cleaning up and concentration of the extract on a silica gel solid-phase extraction column. Two-dimensional thin-layer chromatography on a silica plate was used for the detection of the tranquillizers. Detection levels were 25 micrograms kg-1 for propiopromazine, 50 micrograms kg-1 for azaperone (or its metabolite azaperol) and 125 micrograms kg-1 for carazolol. In pigs treated with the usual doses the presence of propiopromazine and azaperol could be established in kidney tissue 8 h after administration, whilst in injection sites all three tranquillizers could be detected.
Subject(s)
Adrenergic beta-Antagonists/analysis , Azaperone/analysis , Butyrophenones/analysis , Promazine/analogs & derivatives , Propanolamines/analysis , Tranquilizing Agents/analysis , Adrenergic beta-Antagonists/pharmacokinetics , Animals , Azaperone/pharmacokinetics , Chromatography, Thin Layer , Indicators and Reagents , Promazine/analysis , Promazine/pharmacokinetics , Propanolamines/pharmacokinetics , Spectrophotometry, Ultraviolet , Swine , Tranquilizing Agents/pharmacokineticsABSTRACT
A simple method to determine the beta-blocker carazolol in swine kidneys is presented. Spiked samples, homogenised under alkaline conditions, were heated. Passage of the ether extracts through C18 and silica Sep-Pak cartridges yielded a fraction containing the carazolol in 88% recovery. In this fraction carazolol was determined by fluorescence spectrophotometry. By this method a level of 1 microgram/kg in spiked samples can be detected. Injection of 10 micrograms/kg body weight, 105 minutes before slaughter, resulted in carazolol contents in kidneys ranging from 11 to 25 micrograms/kg (n = 8).
