1.
Protein & Cell
; (12): 833-843, 2015.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-757189
ABSTRACT
The protein apoptotic protease activating factor 1 (Apaf1) is the central component of the apoptosome, a multiprotein complex that activates procaspase-9 after cytochrome c release from the mitochondria in the intrinsic pathway of apoptosis. We have developed a vital method that allows fluorescence-activated cell sorting of cells at different stages of the apoptotic pathway and demonstrated that upon pharmacological inhibition of Apaf1, cells recover from doxorubicin- or hypoxia-induced early apoptosis to normal healthy cell. Inhibiting Apaf1 not only prevents procaspase-9 activation but delays massive mitochondrial damage allowing cell recovery.
Subject(s)
Humans , Adenosine Triphosphate , Metabolism , Apoptosis , Apoptotic Protease-Activating Factor 1 , Genetics , Metabolism , Cell Hypoxia , Cell Line, Tumor , Doxorubicin , Pharmacology , HeLa Cells , Microscopy, Electron, Transmission
2.
ACS Med Chem Lett
; 3(7): p.540-3, 2012.
Article
in English
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP
| ID: but-ib8207