ABSTRACT
Infections with Encephalitozoon (phylum Microspora) protozoa in immunodeficient patients have emerged worldwide. The prevalence of infections due to Encephalitozoon species in nonclinical populations remains unclear. We conducted a cross-sectional survey of two rural highland villages in Mexico by using monoclonal antibody 3B6 to Encephalitozoon in immunofluorescence to assess the prevalence of Encephalitozoon spores in human stools. We found that 20 (7.84%) of the 255 subjects were positive and that 15 (21.4%) of the 70 households had at least one member who was positive. These results suggest that Encephalitozoon species, most likely Encephalitozoon intestinalis, may be commonly present in community settings.
Subject(s)
Encephalitozoon/isolation & purification , Encephalitozoonosis/epidemiology , Feces/parasitology , Animals , Antibodies, Monoclonal , Cross-Sectional Studies , Female , Fluorescent Antibody Technique , Humans , Male , Mexico/epidemiology , Prevalence , Rural Population , Water SupplyABSTRACT
Using an anti-oocyst wall monoclonal antibody-based immunofluorescence assay, the presence of Cryptosporidium parvum was evaluated in children with diarrhea from rural areas (selected from a door-to-door community survey) and from urban areas (patients attending hospitals) in the State of Puebla, Mexico. Prevalences of 9.4% in a rural population (n = 85) and 29.6% in a hospital-based urban population (n = 81). There was no consistent correlation between water source and other environmental data and the presence of C. parvum in stools. It is concluded that C. parvum may be an important pathogen associated with diarrhea in this Mexican state.