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1.
Curr Opin Chem Biol ; 27: 10-7, 2015 Aug.
Article in English | MEDLINE | ID: mdl-26043278

ABSTRACT

Fluorescent proteins (FPs), such as the Aequorea jellyfish green FP (GFP), are firmly established as fundamental tools that enable a wide variety of biological studies. Specifically, FPs can serve as versatile genetically encoded markers for tracking proteins, organelles, or whole cells, and as the basis for construction of biosensors that can be used to visualize a growing array of biochemical events in cells and tissues. In this review we will focus on emerging applications of FPs that represent unprecedented new directions for the field. These emerging applications include new strategies for using FPs in biosensing applications, and innovative ways of using FPs to manipulate protein function or gene expression.


Subject(s)
Biosensing Techniques/methods , Fluorescent Dyes/chemistry , Green Fluorescent Proteins/chemistry , Molecular Imaging/methods , Optogenetics/methods , Green Fluorescent Proteins/genetics , Protein Binding
2.
Nat Methods ; 12(3): 195-8, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25622108

ABSTRACT

We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.


Subject(s)
Biosensing Techniques/methods , Green Fluorescent Proteins/metabolism , Luminescent Proteins/metabolism , Calcium/metabolism , Calmodulin/metabolism , Caspase 3/genetics , Green Fluorescent Proteins/genetics , HeLa Cells , Humans , Luminescent Proteins/genetics , Molecular Imaging/methods , Protein Multimerization , Red Fluorescent Protein
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