[Changes in the morphofunctional status of the liver in rats with acute tetrachloromethane poisoning and their correction with antihypoxants, antioxidants and actoprotectors]. / Izmeneniia morfofunktsional'nogo sostoianiia pecheni krys pri ostroi intoksikatsii tetrakhlormetanom i ikh korrektsiia antigipoksantami, antioksidantami i aktoprotektorami.

The acute tetrachlormethane intoxication leads to structural and metabolic alterations in the rat liver. Morphological changes include centrolobular necroses and blood stasis in dilated sinusoidal capillaries. Metabolic changes are manifested by an increase in the water content and time of the spin-lattice (T1) and spin-spin (T2) relaxation (this indicates a lesser degree of water structuralization and its enhanced lability), and distortion of the correlation between T1 and T2. The prophylactic administration of one of the antihypoxants, antioxidants or actoprotectors normalizes the morphofunctional condition of the liver. According to the degree of the therapeutic efficacy, the examined preparations form the following series: dibunol > gamma-sodium hydroxybutyrate > tomerzol.

[Electron microscopic morphometric study of activated chromatin of lymphocytes. I. Variation of chromatin condensations during early action of phytohemagglutinin]. / Elektronno-mikroskopicheskoe morfometricheskoe izuchenie aktivirovannogo khromatina limfotsitov. I. Izmenenie stepeni kondensatsii khromatina v rannii period deistviia fitogemaggliutinina.

We made a quantitative electron microscopic investigation of rat blood lymphocyte chromatin on cell sections in the early period of action of phytohaemoagglutinin (PHA). According to the dynamics of binding of acridine orange to chromatin, there were chosen time lapse after the beginning of stimulation of cells. In one hour of PHA action when the binding of acridine orange is intensified two-fold the total area of profiles of condensed chromatin was not changed, however, the area of membrane-free chromatin profiles was simultaneously diminished by 36%. At that period, the packing of DNP-fibers of condensed chromatin loosened. After 1.5 hr, parallel to a noticeable decrease of binding of acridine orange, there were observed tendencies for restoration of area of chromatin profiles and partial diminishing of relative distances between DNP-fibers. In 6 hrs, the binding of acridine orange was sharply intensified, which was accompanied by a decrease by 26% of the total area of chromatin profiles at the expense of diminishing of perimembranous chromatin. At the same time, the low-electron--density regions of condensed chromatin were enlarged, and the packing of DNP-fibers was strongly loosened.