The Brassica napus blackleg resistance gene LepR3 encodes a receptor-like protein triggered by the Leptosphaeria maculans effector AVRLM1.

LepR3, found in the Brassica napus cv 'Surpass 400', provides race-specific resistance to the fungal pathogen Leptosphaeria maculans, which was overcome after great devastation in Australia in 2004. We investigated the LepR3 locus to identify the genetic basis of this resistance interaction. We employed a map-based cloning strategy, exploiting collinearity with the Arabidopsis thaliana and Brassica rapa genomes to enrich the map and locate a candidate gene. We also investigated the interaction of LepR3 with the L. maculans avirulence gene AvrLm1 using transgenics. LepR3 was found to encode a receptor-like protein (RLP). We also demonstrated that avirulence towards LepR3 is conferred by AvrLm1, which is responsible for both the Rlm1 and LepR3-dependent resistance responses in B. napus. LepR3 is the first functional B. napus disease resistance gene to be cloned. AvrLm1's interaction with two independent resistance loci, Rlm1 and LepR3, highlights the need to consider redundant phenotypes in 'gene-for-gene' interactions and offers an explanation as to why LepR3 was overcome so rapidly in parts of Australia.

Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line.

Isolated microspore culture techniques are being widely used in Brassica breeding programs to generate haploid and doubled haploid plants. A number of factors influence regeneration response in vitro including genotype. In order to assess the effect of genotype on microspore embryogenesis in B. rapa L. var. oleifera, 17 cultivars and breeding lines were evaluated. Embryos developed from all but one genotype when using NLN medium with 17% sucrose, followed by a reduction in sucrose concentration to 10%, 48 h later. The number of embryos /100 buds differed between genotypes, ranging from 0 to 70. Further studies indicated that sucrose concentration and incubation time influenced embryogenesis. Selection studies carried out with an Agriculture and Agri-Food Canada breeding line have resulted in the identification of a highly embryogenic B. rapa line. This line produced thousands of microspore-derived embryos /100 buds and will be useful in mutant selection and gene transfer as well as biochemical and developmental studies.

High-average-power operation of a Q-switched diode-pumped holmium laser.

We have investigated high-peak- and high-average-power operation of diode-pumped, thulium-sensitized, holmium 2.1-microm lasers. Free-running laser powers of 14 W at 29 Hz have been demonstrated with 2.6% electrical efficiency. Q-switched operation produced average powers in excess of 11 W in a burst of short pulses. Preliminary optical parametric oscillator frequency conversion of the holmium laser to 4 microm is also reported.

Spatial attention in Ponzo-like patterns.

A variant of Ponzo's pattern was devised by drawing converging oblique lines at the ends of each of two horizontal lines that were located directly one above the other. Pairs of converging or diverging fins (either between or outside the horizontal shafts) were systematically removed. The results showed that, as predicted by integrative field theory, oblique lines between the shafts produced more distortion than did oblique lines outside the shafts. Also, it was shown that the attentive field construct in integrative field theory was crucial for predicting patterns of means and variances in this experiment. The modified Ponzo effect was shown to have much in common with the classical Müller-Lyer illusion. The subtle differences between the two were explained by proposing that the size of the optimum attentive field was larger in the Ponzo tasks than in the Müller-Lyer tasks because expansion fins are found on both shafts in the Ponzo-like figure.

In vitro culture of isolated microspores and regeneration of plants in Brassica campestris.

A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microspores were isolated from buds 2.0 - 2.9 mm in length and cultured in modified Lichter (1982) medium containing 17% sucrose, pH 6.2. After 48 h at 32°C, the incubation medium was replaced with NLN (Lichter 1982) medium containing 10% sucrose. Microspores were cultured at 24°C in darkness and embryos developed after three weeks. More than 1000 plants have thus far been regenerated. Genotypic differences were observed for microspore embryogenesis. The majority of the regenerants were haploid, however colchicine could be effectively used to achieve chromosome doubling.

Impact of group homes on the values of adjacent residential properties.

The economic impact of locating small group homes in various single-family residential neighborhoods was assessed. Sales within 229 m of 19 homes, which occurred 24 months prior and subsequent to licensing, were examined using a computer assisted mass appraisal model and an adaptive estimation procedure. The findings confirm earlier studies that refute the notion that such homes adversely impact real estate values of adjacent properties. An extensive review of relevant literature and litigation was provided.

Critical depth for the survival of coral islands: effects on the hawaiian archipelago.

Coral islands drown when sea level rise exceeds the maximum potential of coral reefs to grow upward (about 10 millimeters per year). During the Holocene transgression (18,000 years ago to present) sea levels rose at rates of up to 10 to 20 millimeters per year, and most coral island reefs situated deeper than a critical depth of30 to 40 meters below present day sea level drowned. Coral islands that did not drown during the Holocene transgression apparently all developed on antecedent foundations shallower than critical depth. During low stands in sea level during the Pleistocene, these islands were elevated and subject to subaerial erosion. Today, in the Hawaiian Archipelago, the depth of drowned banks is inversely related to summit area; smaller banks are progressively deeper, evidently because of erosional truncation during low sea level stands. Bank summit area may therefore be an important factor determining the failure or success of coral islands.

Effect of ethylene on sanguinarine production from Papaver somniferum cell cultures.

A Papaver somniferum cell line capable of producing sanguinarine equivalent to 3% of cell dry weight was used to determine if ethylene was involved in signalling the biosynthesis of this alkaloid. A 3.3-fold increase in ethylene emanation from these cell suspension cultures was observed 7 h after elicitation with a Botrytis fungal homogenate. The rate of ethylene release then decreased to near zero after 48 h, suggesting that a pulse of ethylene production may be involved in sanguinarine production. However, sanguinarine biosynthesis was not promoted when either the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), or the ethylene releasing agent, 2-chloroethylphosphonic acid (ethephon), was added to the culture. These results strongly suggest that ethylene is not intimately involved in the production of sanguinarine from Papaver somniferum cell cultures or in the transduction of the elicitation event.