ABSTRACT
Malus floribunda Siebold ex Van Houtte is a plant planted for landscaping, and its sour and red fruits have been seen to be frequently used in the treatment of diabetes, making vinegar marmalade, and producing natural food dyes. Apart from these usage areas of this plant, it is aimed at determining the phytochemical content. For this purpose, plant parts (fruit, leaf, and branch) were examined. The antioxidant capacity (vitamins A, E, and C, lycopene, beta-carotene, total phenolic and flavonoid amounts, and DPPH radical scavenging effect), antimicrobial activity (agar well diffusion method, minimum inhibitory concentration-MIC), and GC-MS contents of plant parts were determined. High-performance liquid chromatography (HPLC), spectrophotometers, and gas chromatography/mass spectroscopy (GC-MS) methods were used in the study. It was determined that M. floribunda fruit is rich in lycopene, beta-carotene, and antioxidant vitamins and contains many biomolecules. In addition, it was concluded that the extracts of different parts of the plant have antimicrobial activity. This study has revealed the idea that this plant, whose phytochemical, antioxidant, and antimicrobial content has been determined, can be used as a bioactive substance equivalent to antibiotics in medicine, the food industry, and human nutrition. In addition, it is expected that the study will contribute to the plant literature. Molecular docking studies were performed to evaluate the binding interactions between the compound and human peroxiredoxin 5 and S. aureus. Both in vitro and in silico results indicated that synthesized extracts could act as potent antioxidant and antimicrobial agents.
ABSTRACT
OBJECTIVES: This work provides to evaluate cholesterol assimilation and folic acid production by determining the probiotic properties of Lactobacillus spp. from raw goat milk with prebiotic properties. RESULTS: We isolated Lactobacilli from goat milk and identified API 50, CHL, and 16sRNA. Probiotic properties were determined according to bile salt and acidic tolerance, hydrophobicity, hemolytic activity, antibiotic sensitivity, antagonistic effect, and exopolysaccharide production. In addition, the cholesterol assimilation and folate production of cultures were determined. CONCLUSIONS: L. plantarum GM-12 and L. plantarum GM-15 showed the highest folate production and the highest cholesterol assimilation.These two strains are strong candidates for use as potential probiotics and starter cultures.
Subject(s)
Lactobacillus , Probiotics , Animals , Milk , Prebiotics , Goats , CholesterolABSTRACT
Cyanobacteria are among the beneficial and environmentally friendly natural candidates used in the biosynthesis of nanoparticles, with their ability to accumulate heavy metals from their environment, thanks to their biologically active compounds. In the current study, an aqueous extract of Oscillatoria princeps fresh biomass was used for the green synthesis of AgNPs. UV-vis spectrum, Fourier transforms infrared, scanning electron microscopy, and energy-dispersive spectroscopy were used to validate and characterize biosynthesized of OSC-AgNPs. The biosynthesis of AgNPs was visually verified in terms of the change in the color of the AgNO3 solution from yellowish brown to brown colors from 72 h onwards. An absorption peak of approximately 420 nm was detected in the UV-vis spectrum, corresponding to the plasmon resonance of AgNPs. FT-IR analysis showed the presence of free amino groups in addition to sulfur-containing amino acid derivatives that act as stabilizing agents. SEM images detected the roughly spherical shape of OSC-AgNPs with an average size of 38 nm. The pathogens tested were all susceptible to OSC-AgNPs showing varying antimicrobial effects on pathogenic microorganisms. E. coli and C. albicans displayed the maximum susceptibility, with zones of inhibition of 14.6 and 13.8 mm at 3-mM concentration, respectively, while B. cereus had the lowest zone of inhibition (10.6 mm) at 3-mM OSC-AgN03 concentration. In conclusion, AgNPs synthesized from Oscillatoria princeps inhibit biofilm formation, suggesting that AgNPs may be a promising candidate for the prevention and treatment of biofilm-associated infections caused by bacteria and yeasts.
