ABSTRACT
AIM: To study relationship between Yersinia pseudotuberculosis cultivation temperature and immunobiological properties of lypopolysaccharide (LPS). MATERIALS AND METHODS: LPS producing strain--Yersinia pseudotuberculosis 164/84 OX; experimental animal--guinea pigs, rabbits; methods--immunochemical (indirect hemagglutionation assay, RDP), physico-chemical (electrophoresis, gas-liquid chromatography), standard biochemical and statistical methods. RESULTS: It was established that increase of Yersinia pseudotuberculosis cultivation temperature from 10 degrees C to 37 degrees C was associated with attenuation of LPS toxicity in guinea pigs, decrease of its immunochemical activity and contents and degree of acylation of 3OH-14:0 hydoxyacid as well as amount of electrophoretically detected O-side chains. It was assumed that the polysaccharide component plays its role in LPS toxicity. CONCLUSION: Relation between Yersinia pseudotuberculosis cultivation temperature and structural and functional properties of LPS preparations obtained from the culture was determined.
Subject(s)
Lipid A/immunology , Yersinia pseudotuberculosis/growth & development , Yersinia pseudotuberculosis/immunology , Acylation , Alcohol Oxidoreductases/metabolism , Animals , Chemical Precipitation , Guinea Pigs , Immune Sera/immunology , Lethal Dose 50 , Lipid A/chemistry , Lipid A/toxicity , Rabbits , TemperatureABSTRACT
Instrumental methods of investigation were used for the demonstration of changes in the fatty acid composition of F. tularensis, strain 15 Gaiskii, during cultivation in solid culture medium, storage after lyophilization, as well as changes in the functioning of the system of membrane-dependent enzymes of the respiratory chain and in the permeability of cell wall membranes by water molecules and NADH after lyophilization. A relationship between the survival rate of F. tularensis cells after lyophilization and stimulation of their endogenic respiration with NADH and succinate was revealed. An increase in residual moisture from 6 to 10-12% was found to intensify the process of lipid peroxidation during the storage of lyophilized F. tularensis cells of strain 15 Gaiskii.
