Subject(s)
Acne Vulgaris/enzymology , Glutathione Peroxidase/blood , Adolescent , Adult , Aged , Female , Humans , Male , Middle AgedABSTRACT
The aim of this study was to investigate the effect of administering Glurenorm (gliquidone, 10 mg/kg) on the lenses and skins of streptozotocin-induced diabetic rats. The drug was given to both diabetic and control rats daily, until the end of the experiment, at day 42. The drug was administered to one diabetic and one control group from day 0 and for the other diabetic and control groups from day 14. On day 42, cardiac blood samples, skin samples, and lenses were taken from each rat. Blood glucose (BG) was measured by the o-toluidine method. The total protein, nonenzymatic glycosylation of proteins (NEG), lipid peroxidation (LPO), and glutathione (GSH) levels in the lens and skin homogenates were determined by the Lowry, thiobarbituric acid, Ledwozwy, and Ellman methods, respectively. Laemmli SDS polyacrylamide gel electrophoresis was also carried out on the lens or skin homogenates. After 42 d, Glurenorm given to the diabetic rats produced (i) significant reductions in BG, NEG, and total protein in the lenses; (ii) significant increases in GSH levels in the lenses; (iii) and no significant results in the skin. The body weights of the drug group dropped relative to day 0, but not significantly. SDS polyacrylamide gel electrophoresis revealed no significant differences in any of the protein bands between any of the groups. In the lenses, the gains in turns of reduced NEG and increased GSH may have been offset by the reduction in protein.
Subject(s)
Diabetes Mellitus, Experimental/drug therapy , Glutathione/drug effects , Hypoglycemic Agents/pharmacology , Lens, Crystalline/drug effects , Lipid Peroxidation/drug effects , Skin/drug effects , Sulfonylurea Compounds/pharmacology , Animals , Blood Glucose/drug effects , Blood Glucose/metabolism , Diabetes Mellitus, Experimental/chemically induced , Diabetes Mellitus, Experimental/metabolism , Disease Models, Animal , Female , Glutathione/metabolism , Glycosylation/drug effects , Lens, Crystalline/metabolism , Rats , Skin/metabolism , StreptozocinABSTRACT
Parsley (Petroselinum crispum) is one of the medicinal herbs used by diabetics in Turkey and it has been reported to reduce blood glucose. The purpose of this study therefore was to investigate the effect of feeding parsley on diabetes induced impairments in rat skins. Uncontrolled induced diabetes caused significant increases in nonenzymatic glycosylation of skin proteins, lipid peroxidation and blood glucose. Administration of parsley extract did not inhibit these effects except for the increase in blood glucose. SDS-polyacrylamide gel electrophoresis revealed no significant differences in any protein bands between any of the groups.
Subject(s)
Apiaceae , Diabetes Mellitus, Experimental/metabolism , Diabetes Mellitus, Type 1/metabolism , Hypoglycemic Agents/pharmacology , Skin/drug effects , Animals , Apiaceae/therapeutic use , Blood Glucose/drug effects , Diabetes Mellitus, Experimental/chemically induced , Diabetes Mellitus, Experimental/therapy , Diabetes Mellitus, Type 1/chemically induced , Diabetes Mellitus, Type 1/therapy , Electrophoresis, Polyacrylamide Gel , Glycosylation/drug effects , Hypoglycemic Agents/therapeutic use , Lipid Peroxidation/drug effects , Male , Phytotherapy , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Rats , Streptozocin , TurkeyABSTRACT
Chard (Beta vulgaris L. var. cicla) is one of the plants used as hypoglycaemic agent by diabetics in Turkey and it has been reported to reduce blood glucose. The purpose of this study was to investigate the effect of feeding chard on diabetes induced impairments in rat skins. Uncontrolled induced diabetes caused significant increases in nonenzymatic glycosylation of skin proteins, lipid peroxidation and blood glucose. Administration of chard extract inhibited these effects except the increase in lipid peroxidation. SDS-polyacrylamide gel electrophoresis revealed no significant differences in any protein bands between any of the groups. The data indicate that the use of chard may be effective in preventing or at least retarding the development of some diabetic complications.