Chronology of PAH and PCB pollution using sediment core in the Golden Horn estuary (Sea of Marmara).

The sources and depositional history of 16 polycyclic aromatic hydrocarbons (PAHs) and 18 polychlorinated biphenyl (PCBs) congeners in the Golden Horn estuary (Istanbul) were investigated using a dated sediment core for the period between 1880 and 2012. The concentrations of PAHs and PCBs were calculated for every 4 cm slices of the sediment core and ranged from 1203.5 to 3441.4 ng/g and 5.4 to 41.4 ng/g, respectively. The diagnostic ratios indicated that the maximum PAH values correspondence to combustion after a crude oil-carrying Romanian tanker (Independenta) accident in the Istanbul Strait in 1979. The historical deposition of PAHs and PCBs in the Golden Horn was influenced by municipal effluent and heavy industrial dischargers approximately 50 years. When the Silahtaraga thermal power plant (TPP) was operating, PCB pollution rose; however, after a thorough rehabilitation effort and the outlawing of PCB use in the 1990s, pollution levels significantly tended to decrease.

Desflurane anaesthesia increases sister chromatid exchanges in human lymphocytes.

BACKGROUND: We investigated genotoxic effects of desflurane on the frequency of sister chromatid exchange (SCE) in peripheral blood lymphocytes of patients during and after anaesthesia. METHODS: Fifteen female patients, ASA classification I-II, aged 26-54 years, undergoing elective surgery were enroled in this study. Anaesthesia was induced by injection of thiopental 5-7 mg/kg and fentanyl 1 microg/kg. Vecuronium 0.1 mg/kg was given to facilitate tracheal intubation. Anaesthesia was maintained with desflurane 5-6% in an oxygen/air mixture (FiO(2) 0.3). N(2)O was not used for any patient. Using a heparinized syringe, venous blood was collected in patients before anaesthesia. Additional venous blood samples were taken from all patients at 60 and 120 min after the initiation of anaesthesia. Post-operative blood samples were taken and first, third, seventh and twelfth day samples were coded. RESULTS: Number of SCEs per cell at 60 and 120 min were significantly higher than the number of SCEs per cell before anaesthesia. In addition, number of SCEs per cell at 1, 3 and 7th post-operative days were significantly higher than pre-operative levels (P < 0.05). There was no difference between pre-operative number of SCEs per cell and 12th post-operative day levels (P > 0.05). CONCLUSION: In the present study, because exposure to desflurane increased sister chromatid exchange in human lymphocytes in our group of patients, we conclude that this agent may be capable of producing genetic damage.

The Fibrin Assay Comparison Trial (FACT): evaluation of 23 quantitative D-dimer assays as basis for the development of D-dimer calibrators. FACT study group.

Although D-dimer has gained widespread clinical use as a parameter for detection of in vivo fibrin formation, the issue of standardization of D-dimer assays remains to be resolved. The FACT study was performed to generate basic data for development of calibrators and standard preparations. A set of 86 samples, including plasma samples from patients with DIC, DVT. and other clinical conditions, serial dilutions of pooled plasma samples, and plasma samples containing fibrinogen- and fibrin derivatives, were distributed to 12 manufacturers of D-dimer assays. D-dimer assays differ concerning specificity for crosslinked fibrin, and preference for either high molecular weight fibrin complexes, or low molecular weight fibrin degradation products. Terminal plasmin digests of fibrin clots for calibration produce aberrant results in some assays, especially those with preference for high molecular weight crosslinked fibrin derivatives. The best conformity is achieved by the use of pooled plasma samples from patients with high levels of D-dimer antigen in plasma. In vitro preparations containing a comparable composition of fibrin derivatives to clinical plasma samples may also serve as reference material.

The fibrin assay comparison trial (FACT): correlation of soluble fibrin assays with D-dimer.

Soluble fibrin (SF) is regarded as an indicator of acute fibrin formation and a precursor of fibrin thrombi. Using a set of clinical plasma samples, and fibrin derivatives, five assays for measurement of SF, including two chromogenic assays, two ELISA systems, and one latex-enhanced photometric immunoassay were compared. Correlation between SF assays was moderate (Spearman's rho values between 0.344 and 0.805). Re-calibration with serial dilutions of desAABB-fibrin monomer resulted in adjustment of the numerical scale of the assays without improving correlation. All SF assays reacted with purified crosslinked fibrin derivatives. Using clinical plasma samples, Spearman's rho of SF assays with D-dimer consensus values based upon results of 23 quantitative D-dimer assays were between 0.491 and 0.911. Although all SF assays react with desAABB-fibrin monomer complexes, SF assays are heterogeneous concerning reactivity with fibrin compounds observed in clinical plasma samples. The prospect of a common calibrator for SF assays therefore seems to be remote. Since SF assays react with crosslinked fibrin derivatives, it is not possible to clearly distinguish between acute fibrin formation, and fibrin dissolution on the basis of the results of current SF assays.