[Combined diagnosis of primary lung cancer using immunological tests]. / Kompleksnaia diagnostika pervichnogo raka legkogo s ipol'zovaniem immunologicheskikh issledovanií.

To compare the accuracy of diagnosis obtained with different diagnostic techniques the authors examined 187 patients with cancer of the lung clinically, roentgenologically, bronchologically, morphologically and immunologically. X-ray made the diagnosis of lung cancer in 85% of the examinees. This diagnosis was confirmed in 87% of the cases. Morphological verification was obtained in 84.4% and 78.2% of patients with central and peripheral cancer, respectively. Additional immunological investigations increased the proportion of accurate diagnoses up to 96.8%. It is concluded that immunological investigations are effective in complex diagnosis of lung cancer.

[Delayed-type hypersensitivity skin test with modified autologous lymphocytes in the diagnosis and monitoring of patients with lung cancer]. / Kozhnaia reaktsiia GZT s autologichnymi modifitsirovannymi limfotsitami v diagnostike i monitoringe bol'nykh rakom lëgkogo.

The delayed-type hypersensitivity skin test was run with autologous modified lymphocytes, treated with phytohemagglunin, in 218 patients suffering different lung diseases. The three following groups were identified: lung cancer, chronic non-specific disease of the lung and benign lesions in the lung. Cancer patients proved positive to autologous modified lumphocytes in 90.4%. Skin reaction was close to normal in the other groups. Skin reaction dynamics was compared in cases of recurrence and recurrence-free patients in the group of surgical treatment for lung cancer.

[A possible role of phosphoprotein p53 in the mechanism of autostimulation of tumor cell proliferation]. / Vozmozhnaia rol' fosfobelka p53 v mekhanizme autostimuliatsii proliferatsii opukholevykh kletok.

It has been shown that phosphoprotein p53 may participate in the DNA-synthesis stimulation in the mixed culture of tumour cells and syngeneic embryonic mouse cells (10 days of pregnancy). This effect has been eliminated by the preliminary treatment of embryonic cells in vitro by the monoclonal antibodies to p53 (pAb421) in the model system. A conclusion is drawn that p53-epitopes of embryonic cell surface membrane take part in the formation of the determinant which is recognized by receptors of tumour cells. The stimulation of DNA-synthesis in tumour cells is resulted by this process.

[Possible role of embryonic surface antigens in forming the autostimulus of tumor cell proliferation]. / Vozmozhnaia rol' émbrional'nykh poverkhnostnykh antigenov v formirovanii autostimula proliferatsii opukholevykh kletok.

It is shown that DNA synthesis intesifies in a mixed culture of cells of the tumours of different histogenesis, induced by monkey adenovirus SA7(C8) and chemical carcinogens, with syngenic and allogenic cells of an early embryo. This intensification of the synthesis in tumour cells is due to the contact of surfaces participating in the cell response but not to the soluble factors because when the embryo cells are placed into the milliporous chamber, the described effect is inhibited.

[Immunity to embryonal stage-specific antigens in viral carcinogenesis]. / Immunitet k embrional'nym stadiospetsificheskim antigenam pri virusnom kantserogeneze.

The oncogenesis induced by the monkey SA7(C8) adenovirus in CBA/Ca mice has shown that immune responses to embryonal antigens are formed at early stages of the latent period and are preserved for a long time reaching the maximum by the 30th day of the latent period. The observed immune response to early embryonal antigens is considered as a factor of the tumour growth immunostimulation and also as a condition limiting the antitumour immunity.

[Antitumor effect of a tumor-cell neuraminidase vaccine]. / Protivoopukholevyi éffekt neiraminidaznoi vaktsiny opukholevykh kletok.

The effect of neuraminidase vaccine of CBA mouse sarcoma cells caused by simian adenovirus SA7 (C8) on growth parameters of transplanted sarcoma in a syngeneic system was studied. Inoculation of the vaccine was found to inhibit tumor growth. This effect was more marked in a group of animals given tumor cells after preliminary vaccination. There was no correlation between tumor growth parameters and cytotoxic activity of spleen cells assesses in the cytotoxicity test by 51Cr release in the groups of vaccinated and control animals. It is concluded that the treatment of tumor cells with neuraminidase increases their immunogenicity. The cytotoxic activity of spleen cells in vaccinated animals appears earlier and persists longer.

[Changes in the cyclic AMP level in the cells of murine sarcoma induced by monkey adenovirus SA7(C8) during tumor growth enhanced by lymphocytes from intact syngeneic mice]. / Izmenenie urovnia TsAMP v kletkakh sarkomy nyshei, indutsirovannoi obez'ian'im adenovirusem SA7(C8), v dinamike rosta opukholi, uskorennogo limfotsitami ot intaktnykh singennykh myshei.

Under consideration is the problem of changes in the cAMP level in sarcoma of mice CBA with routine and enhanced rate of growth. To this end the kinetics of sarcoma growth, induced by monkey adenovirus SA7 (C8), with common and stimulated by lymphocytes from intact syngeneic animals rates of growth was studied. Simultaneously, the kinetics of intracellular cAMP content in tumor cells was studied too. It was found that there is an inverse dependence between the cAMP content and the rate of sarcoma SA7 (C8) growth. Use of one type of tumor cells with the predetermined different rate of growth makes it possible to relate the changes in the content of intracellular tumor cell cAMP with changes being due to growth potentials.

[Mechanisms of changes in the mass of the organs central to immunity during adenovirus carcinogenesis]. / O mekhanizmakh izmeneniia massy tsentral'nykh organov immuniteta pri adenovirusnom kantserogeneze.

The spleen cells transfer from mice CBA at the 25th day of the carcinogenesis latent period induced by adenovirus SA7(S8) to newborn syngeneic animals caused the graft versus host reaction in them. There was splenomegaly and progressive decrease in weight of the recipients' thymus. Analogous alterations of lymphoid organs were noted in the animals infected during the neonatal period by oncogenic adenovirus SA7(C8). Results showed that adenoviral carcinogenesis had some manifestations of autoimmune disease.

[Immunostimulation of the growth of a syngeneic sarcoma primarily induced in mice by simian adenovirus SA7(C8)]. / Immunostimuliatsiia rosta singennoi sarkomy, pervichno indutsirovannoi u myshei adenovirusom obez'ian SA7(C8).

Transplantation of splenic cells from CBA mice bearing primary sarcoma SA7(C8) together with autologous sarcoma cells (in the ratio 1 : 1 and 10 : 1) into syngeneic recipients, irradiated with 600 rad, resulted in a marked decrease of the latent period of tumor development. Syngeneic splenocytes of normal mice would enhance the tumor growth with the ratio 10 : 1. Preliminary treatment of tumor-bearing and normal mice with hydrocortisone in the dosage of 6 mg per 100 g of weight fails to reduce the capacity of splenic cells to induce immunostimulation of tumor growth.

[Splenocyte autoreactivity of mice of sensitive lines during the latent period of carcinogenesis induced by virus SA7(C8)]. / Autoreaktivnost' splenotsitov myshei chuvstvitel'nykh linii v latentnom periode kantserogeneza, indutsirovannogo virusom SA7(C8)

The method of adsorption on a monolayer of embryonal fibroblasts of 51Cr-labelled spleen cells from CBA and C57B1/6 mice infected at birth with SA7(C8) virus was used to show that the cells immune to the antigens of mouse embryo fibroblasts accumulated in the spleen of CBA mice highly susceptible to oncogenesis induced by SA7(C8) virus. In the controls, splenocytes of intact CBA mice were adsorbed on embryonal fibroblast monolayers (the percentage of adsorption 9.8 and 4.2, respectively; P less than 0.05). In the spleen of C57B1/6 mice insusceptible to oncogenesis induced by SA7(C8) virus there was no accumulation of cell antibody to the antigens of embryonal fibroblasts (P greater than 0.1). The detected antibody caused no cytotoxic effect on embryo fibroblasts.