ABSTRACT
A biologically active composition of chitosan-selenium nanoparticles has been developed. Selenium nanoparticles are characterized by a clear bimodal size distribution: 2-3 and ~37 nm. The main active centers of complexation with nanoparticles are the amino and hydroxyl groups of chitosan. In experiments on culturing fibroblasts of the hTERT BJ-5ta cell line on sample films, high biocompatibility of the composition was shown. It was shown that the composition of chitosan-selenium nanoparticles has a corrective effect on the oxidative processes of the body, reducing the activity of free-radical oxidation in the blood of animals. This opens up prospects for the use of this complex in the composition of antioxidant and adaptogenic drugs.
ABSTRACT
The review is devoted to the systematization, classification, and generalization of the results of modern scientific research on the role of bile acids as a new class of steroid hormones. The paper presents the evidence for bile acid participation in the regulation of the body energy metabolism, body weight control, as well as the pathogenesis of obesity, diabetes mellitus, insulin resistance, and cardiovascular diseases. Particular attention is paid to the role of bile acids in the control of nonspecific energy expenditure of the body. The applied aspects of using the novel data about the membrane and intracellular receptors responsible for the development of hormonal regulatory effects of bile acids are analyzed. According to the authors, the modern data on the role of bile acids in the regulation of body functions allow a deeper understanding of the pathogenesis of body weight disorders and associated cardiovascular diseases. The review demonstrates promising directions in the search for specific methods of prevention and correction of these pathological conditions.
Subject(s)
Bile Acids and Salts , Insulin Resistance , Energy Metabolism , Hormones , Humans , ObesityABSTRACT
This review presents the analysis and systematization of modern data on the molecular mechanisms of autism spectrum disorders (ASD) development. Polyetiology and the multifactorial nature of ASD have been proved. The attempt has been made to jointly review and systematize current hypotheses of ASD pathogenesis at the molecular level from the standpoint of aberrant brain plasticity. The mechanism of glutamate excitotoxicity formation, the effect of imbalance of neuroactive amino acids and their derivatives, neurotransmitters, and hormones on the ASD formation have been considered in detail. The strengths and weaknesses of the proposed hypotheses have been analyzed from the standpoint of evidence-based medicine. The conclusion has been drawn on the leading role of glutamate excitotoxicity as a biochemical mechanism of aberrant neuroplasticity accompanied by oxidative stress and mitochondrial dysfunction. The mechanism of aberrant neuroplasticity has also been traced at the critical moments of the nervous system development taking into account the influence of various factors of the internal and external environment. New approaches to searching for ASD molecular markers have been considered.
Subject(s)
Autism Spectrum Disorder , Amino Acids , Autism Spectrum Disorder/genetics , Glutamic Acid/metabolism , Humans , Neuronal Plasticity , Oxidative StressABSTRACT
The course of preventive oral treatment with a dihydroquercetin-chitosan composition produced a strong antihypoxic effect under conditions of experimental hypobaric hypoxia (arbitrary altitude 12,000 m). The lactate/pyruvate ratio in composition-receiving rats was much lower than in hypoxic animals (by 83%), but higher than in intact specimens (by 29%). The composition of chitosan and dihydroquercetin also possessed a strong antioxidant activity.
Subject(s)
Chitosan/pharmacology , Hypoxia/metabolism , Models, Biological , Quercetin/analogs & derivatives , Acute Disease , Animals , Chitosan/chemistry , Male , Quercetin/chemistry , Quercetin/pharmacology , RatsABSTRACT
The objective of the present study was to calculate the blood ethanol level from its content in the exhaled air. The plot of the blood ethanol level versus its content in the exhaled air was constructed and used to determine the rate of ethanol elimination from the blood. The result proved to lie within the range corresponding to the normal-for-age values. These data put in question the opinion of the independent specialist about disturbances in the alcohol dehydrogenase activity in blood manifested as a considerable increase of the rate of acetaldehyde reduction to ethanol with the decreasing ethanol dehydration rate. It is concluded that the prfoposed algorithm can be recommended for the application in the practical work of various expert services.
Subject(s)
Alcohol Dehydrogenase/blood , Ethanol/blood , Forensic Toxicology/methods , Adult , Breath Tests , Ethanol/analysis , Female , Humans , Inactivation, MetabolicABSTRACT
We showed that mitochondrial creatine kinase activity can be predicted from the ATP level and that changes in ATP content can be evaluated by activity of mitochondrial creatine kinase under experimental conditions.
Subject(s)
Adenosine Triphosphate/metabolism , Brain/physiopathology , Creatine Kinase/metabolism , Energy Metabolism/physiology , Hypoxia/physiopathology , Mitochondria/metabolism , Animals , Brain/metabolism , Hypoxia/metabolism , Male , Models, Biological , Predictive Value of Tests , Rats , Regression AnalysisABSTRACT
The disturbances of blood circulation changes metabolism and energy reactions in the brain. The method of mathematical analysis is used for the study of the dependence between the activity mitochondrial creatine kinase (CK) and ATP level the brains of rats subjected to acute and chronic ischemia. A model describing this dependence under different conditions of the activity of organisms has been proposed. Thus it is possible to predict catalytic properties of CK based on ATP level.
