ABSTRACT
After the accident at the Chernobyl nuclear power plant, a considerable increase in the incidence of thyroid cancer among children in Belarus was observed. In the present study, the frequency of the c-ret protooncogene rearrangements in samples of thyroid carcinomas resected and diagnosed in 1998 from individuals in Belarus was investigated. The ret/PTC1 oncogene was detected in 19% of the samples, and the ret/PTC3r1 oncogene, in 14%. The number of ret/PTC1 rearrangements observed in tumor cells from the patients whose age at the time of the accident was from 1 to 10 years, was greater compared to those whose age at the time was from 10 to 20 years, irrespective of the year of surgery (1996 or 1998). The majority of the patients with ret/PTC3r1 rearrangements lived in Gomel oblast, which was contaminated by the Chernobyl meltdown.
Subject(s)
Gene Rearrangement , Neoplasms, Radiation-Induced/genetics , Oncogene Proteins, Fusion/genetics , Oncogene Proteins/genetics , Power Plants , Radioactive Hazard Release , Thyroid Neoplasms/genetics , Transcription Factors , Child , Humans , Nuclear Receptor Coactivators , Protein-Tyrosine Kinases , Thyroid Neoplasms/etiology , UkraineABSTRACT
The peculiarities of alternative CD44 mRNA splicing in thyroid cancer tissue of children from radiocontaminated areas was investigated. CD44 gene expression in thyroid cancer tissues of children exposed to radiation resembled that in spontaneously emerged cancers. It was concluded that CD44 gene expression is not the primary target of radioactive irradiation. Probably, the CD44 mRNA splicing deregulation is the consequence of cancer.
Subject(s)
Carcinoma, Papillary/genetics , Gene Expression Regulation, Neoplastic/genetics , Hyaluronan Receptors/genetics , Oncogenes/genetics , Thyroid Neoplasms/genetics , Alternative Splicing/genetics , Alternative Splicing/radiation effects , Base Sequence , Child , DNA Primers , Gene Expression Regulation, Neoplastic/radiation effects , Humans , Hyaluronan Receptors/radiation effects , Molecular Sequence Data , Oncogenes/radiation effects , Polymerase Chain Reaction/methods , Power Plants , RNA, Messenger/genetics , RNA, Messenger/radiation effects , Radioactive Hazard Release , Republic of Belarus , Thyroid Gland/radiation effects , UkraineABSTRACT
The DNA sequence (AT)26 of barley genome has been cloned. This sequence is arranged in intraspecific locus and is repeated 1500 times per haploid genome. This fragment is not translated and can form cruciform structures in the AT region.
