N-methyl-trimethylacetamide in thin films displays infrared spectra of π-helices, with visible static and dynamic growth phases, and then a ß-sheet.

The simplest (minimal) peptide model is HCONHCH3. An increase in the π-helix content with increased substitution in the acyl portion suggested the examination of N-methyl-trimethylacetamide) (NMT). NMT displays spectra, in which there is evolution of a set of helices defined by their amide I maxima near 1686 (3(10)), 1655 (first π), and, most importantly, at 1637 cm(-1) (π). Expanded thin-film infrared spectroscopy (XTFIS) shows pauses or slow stages, which are identified as static phases followed by dynamic phases with the incremental gain or loss of a helix turn. In addition, absorbance at 1637 cm(-1) suddenly increases at 82.1 s (30% over 0.3 s), indicating a phase change and crystallization of the π-helix, along with a coincidental decrease in the absorbance for the first π-helix. A sharp peak occurs at the maximum of the phase change at 82.5 s, representing a pure NMT π-helix. The spectra then undergo a decreasing general absorption loss over 150 s, with the π-helix evolving further to an antiparallel ß-sheet fragment. The spectral quality arises from the immobilization of polar molecules on polar surfaces. The crystal structure is that of an antiparallel ß-sheet.

Xanthopterin in the Oriental hornet (Vespa orientalis): light absorbance is increased with maturation of yellow pigment granules.

The Oriental hornet bears both brown and yellow colors on its cuticle. The brown component is contributed by the pigment melanin, which is dispersed in the brown cuticle and provides protection against insolation, while the yellow-colored part contains within pockets in the cuticle granules possessing a yellow pigment. These yellow granules (YG) are formed about 2 days prior to eclosion of the imago, and their production continues for about 3 days posteclosion. Xanthopterin is the main component of the granule and lends it its yellow color. Xanthopterin produces a characteristic excitation/emission maximum at 386/456 nm. Characterization by use of mass spectrometry showed the compound to have a molecular ion of 179, as expected from xanthopterin. Spectroscopic examination of the absorption of an entire stripe of yellow cuticle in the course of its metamorphosis revealed that the absorption steadily increases throughout the process to a maximal level of absorption about 3 days posteclosion. In the absence of the YG, the cuticle is permeable to the passage of all wavelengths within the visible range and to the UV range (290-750 nm) in all age groups of hornets. The newly ecloded hornets depart the nest to engage in activities requiring exposure to insolation only as the process of granule formation terminates, namely, when the layer of YG in the cuticle suffices to absorb all the harmful UV radiation.

Ontogenesis of the thermogenic center in hornets.

In the Oriental hornet, a thermogenic center is located in its prothorax. The present study attempted to elucidate the development of this organ with age, that is, by following the development of the thermogenic center in the hornet from its pupal stage until several days after eclosion of the imago. To this end, use was made of an infrared camera, with which pictures were taken of the prothorax in hornets at various ages, i.e., several days prior eclosion, 24 h after eclosion, and 48-h posteclosion. The photographic findings established that prior to 48-h posteclosion there was no thermally distinct region or spot in the prothorax, but at about 48 h, such a "hot spot," namely, a point whose temperature is greater than that of the rest of the prothorax, does appear, and its appearance coincides with certain specific nest activities like warming of the pupae. Next, an attempt was made to transplant by allograft the region in the prothorax housing the hot spot. Accordingly, several pupae at 2 days prior to eclosion were subjected to the following procedure; their future prothoracic thermogenic center was excised and so also an equally sized piece of cuticle from the dorsal region of their abdomen, and the two now allografted in exchange, i.e., the piece from the prothorax replacing the abdominal piece and vice versa. The result of this exchange-transplant was studied 48 h after eclosion of the operated hornets and showed disruption of heat formation in the prothoracic site coupled with nonappearance of a hot spot in the abdominal site. As for the functional, intact hot spot in the adult hornet, it is characterized by a high concentration of tracheae, with numerous mitochondria in between them that probably contribute to the heat generation.

Prevention of hyperthermia with silk of the oriental hornet, Vespa orientalis: a hypothesis.

Wasps apparently develop normally even under extreme thermal conditions, including deserts. We deemed it worthwhile to set up an experiment wherein wasp brood combs containing a full gamut of brood ranging from eggs up to pupae and a few adults were kept in an incubator whose temperature was gradually raised to 45 degrees C, and the response of the disparate brood to such warming was photographed via Infra Red camera. The finding of this experiment showed that for open brood (i.e., eggs, larvae at various instars, and empty cells) the temperature was close to the ambient temperature, but in the silk coated pupae, the temperature was lower than the ambient by up to 4 degrees C. This lower temperature was retained for at least 90 min of incubation. For comparison we evaluated the relative contribution of the pupae to the phenomenon, by warming also a vacant, (i.e., a broodless and silkless comb) in parallel to a comb from which the pupae had been extricated but the silk weave retained and left behind. We found that the totally empty comb heated up under these conditions to nearly 110 degrees C, whereas the silk-containing vacant cells only heated up to about 40 degrees C. These finding are discussed from two aspects, namely the importance for wasps to maintain a constant temperature throughout the pupating process, and the manner in which the silk weave contributes to such a goal.

Presence of a thermoregulatory hot spot in the prothorax of the large carpenter bee and the bumble bee.

In both the large carpenter bee (Xylocopa pubescens) and the bumblebee (Bombus terrestris), a hot spot was detected in the center of the prothorax on its dorsal-external aspect. In both cases, the temperature in this hot spot was found to be greater than the ambient temperature and that at the tip of the gaster. In B. terrestris, it was higher by 9-10 degrees C from that at the gaster tip and by 15-16 degrees C from the ambient temperature, while in X. pubescens the corresponding differences were 11-20 degrees C and 18-19 degrees C, respectively. The recorded thermal differences were not fixed but were rather variable, temporally as well as individually, but invariably all individuals measured showed these temperature differences. Furthermore, in none of the studied specimens was a hot spot detected in any part of the body other than the prothorax. From this hot spot in the prothorax, there is a cascade of temperatures in both directions, that is, anteriorly towards the head and posteriorly towards the gaster, with a graded drop in temperature in either direction. This article discusses possible reasons for the existence of such a hot spot in this particular location (the prothorax), its role or function, and its mode of operation. The authors speculate that it is a thermoregulatory center (for heating or cooling) that might be present in possibly all Hymenoptera that spend a considerable part of their life flying, regardless of whether they are social, parasocial, or solitary.

The thermogenic center in social wasps.

In the social wasps Vespa orientalis and Paravespula germanica (Hymenoptera, Vespinae), a thermogenic center has been found in the dorsal part of the first thoracic segment. The temperature in this region of the prothorax is higher by 6-9 degrees C than that at the tip of the abdomen, and this in actively flying hornets outside the nest (workers, males or queens) as well as in hornets inside the nest that attend to the brood in the combs. On viewing the region from the outside, one discerns a canal or rather a fissure in the cuticle, which commences at the center of the dorsal surface of the prothorax and extends till the mesothorax. Thus the length of this canal or fissure is approximately 5-7 mm and it is seen to contain numerous thin hairs whose shape varies from that of the hairs alongside the structure. Beneath the cuticle in this region there are dorsoventral as well as longitudinal muscles in abundance, much the same as the musculature in the remaining thoracic segments (i.e. the meso- and metathorax), which activate the two pairs of wings. The canal-bearing segment is of course devoid of wings, and its dorsoventral muscles are attached to the cuticle, which in this region resembles a bowl harboring several layers of epithelium that boasts numerous butterfly-shaped tracheal branches. Additionally there are layers that display lymph-filled spaces and also perforated layers and depressions, and beneath all these is a lace-like layer that also coats the cuticle's hollows. Underneath the cuticle proper, there are numerous large mitochondria and tracheae, which occupy a considerable part of the cuticular epithelium surface. These abundant mitochondria are, most probably, the main element of heat production in the thermogenic center.

Antennal and cephalic organelles in the social wasp Paravespula germanica (Hymenoptera, Vespinae): form and possible function.

This paper deals with hairs and organelles present on the head and antennae of the German wasp, Paravespula germanica, and their possible role in sensing the physical and chemical ambience, as well as in intercommunicating both while in flight outside or in the nest. Via scanning electron microscope photography, we detected on the frons plate of the wasp's head, hairs that were about 300 microm long and comprised the longest hairs on the body of the wasps. Additionally, the two antennae bore along their entire length photoreceptors, placoids, campaniforms, trichoids, and agmons. These organelles are located at high but variable density along the antennal segments. The paper provides the dimensions of each of the mentioned organelles, and discusses the possible functions of the organelles as well as of the hairs on the frons. Photographs taken via atomic force microscope reveal that the epicuticle of the antenna is of two typical shapes; one, bearing both longitudinal stripes as well as transverse bands that are about 1 mum in width, and a second granulated form. Conceivably, the wasp uses the various organelles mentioned to communicate with its mates that are some distance away, somewhat like the use of radar by humans.

Diazepam inhibits reproduction and reproductive behavior of oriental hornet. A possible role for the peripheral-type benzodiazepine receptor.

Feeding of diazepam to young hornets completely inhibits or delays development of their ovaries for a relatively long period. In control hornets, the ovaries usually develop within a day or two post eclosion and comb building commences on the second day of life. The hornets then oviposit into the comb cells and the deposited ova give rise to larvae. Trials were performed on parallel groups of hornets of various ages. When the sedative diazepam was administered to hornets aged 0-24 hours the ovaries of these young hornets failed to show any development, so that no oocytes ripened and consequently there was no oviposition whatsoever. Neither were any comb cells built or, at best, only a few were built. When the diazepam was administered to hornet's being the age of 48 hours, it exerted no change, that is, the eggs developed normally and comb building was the same as in the control group. Longevity of hornets was uniform in all the test groups and similar to that in the control.

MRI of oriental hornet head.

The head of the Oriental hornet in situ, detached from a live sample was imaged using Magnetic Resonance Imaging (MRI). This non-invasive method enabled us to visualize the three-dimensional structure of the hornet's brain and intracerebral organs, as based on cubic voxels of 23 microm3. From these images, we could identify various cephalic structures in both supra-esophageal and sub-esophageal locations. In the former location, we identified and visualized the ocelli, ommatidia, mushroom body, lobula, medulla and the compound eyes in the protocerebrum, as well as the olfactory lobe and bases of the antenna in the deutocerebrum, while in the sub-esophageal region we visualized organs such as the mouthparts, the esophagus, the gnathal pouch and the salivary ducts that empty into the region. Additionally, we identified various muscles, the aorta, cuticular thickenings lending support to the interior of the head and also the cuticular skeleton providing support on the outside. All the mentioned structures and organs were visualized in their relative, normal proportions, without touching or dislocating them.

A thermoregulatory center in hornets: IR photography.

In the Oriental hornet Vespa orientalis (Hymenoptera, Vespinae), there is on the dorsal side of the thorax, beneath the mesoscutum plate of the prothorax and around the median notal suture, a lump that, in the course of hornet activity, is warmer by 9 degrees C from the surrounding milieu and by up to 6 degrees C from other body parts of the hornet. This lump is about 1 mm in diameter, butterfly-shaped, and its upper, posterior border abuts the base of the forewings. During hornet activity and via Infra Red photography one can observe heat extensions stemming from the center of the lump and proceeding forward in the direction of the head, downward toward the legs and backwards toward the bases of the wings. The warmest region is the center of the lump, with its margins showing a lower temperature. As for the legs of the hornet, their upper part is warmer than the other parts. The temperature gradients along the hornet's body are dependent on the extent and nature of hornet activity. Thus, during flight or ventilation activity, the thorax is the warmest part of the body, while the wings, legs, and antennae, as well as the posterior part of the gaster are colder, yet all these body parts are still warmer to varying degrees than the surrounding milieu. Thus, at night, when sentry worker hornets stand guard around the nest entrance and remain practically motionless, the temperature differences between the various body parts are retained unchanged. We conjecture that the described butterfly-shaped lump is a thermoregulatory center (TC), which is neurogenically activated, since the changes occurring in it are rapid, a matter of one to several seconds and do not appear to be directly dependent on the hemolymph supply. The thermoregulatory center keeps a high constant temperature apparently related to hornet activity and the environmental conditions. The temperature cascade is most probably regulated via the tracheal system. Apparently another system activated by a heat pump mechanism keeps the gaster at a lower temperature than the environment.

Cilia in the head of hornets: form and function.

In the head of the Oriental hornet, beneath the cuticle, there are plaques of hair cells. These are distributed throughout the upper front part of the head; to wit: in the region of the vertex (i.e., around and behind the ocelli), in the genae around and behind the compound eyes (the ommatidia), and in the region of the forehead or frons. These hair cells are arranged with their thin whip-like part (i.e., cilia) directed outward and morphologically fall into three distinct groups: type (a) thin elongated cilia connected to each other alongside by side-links; type (b) thin elongated cilia of which two or more interconnect at their distal ends via a delicate nerve fiber bearing a knob at its center; and type (c) shorter and thicker cilia that roughly resemble a triangular thorn and are also interconnected by a thin thread, which, however, bears a ball rather then a knob at its center. The knob in the one case and the ball in the other vary in their diameter, but in both instances the interconnecting elements, be they nerve fibers or threads, are seemingly multidirectional. Beneath the frons, in the region of the coronal suture, the hair cells (cilial plaques) are inwardly directed and bear a large trachea at their center. Presumably, the "weighted" cilial cells that are directed toward the exterior of the body aid the hornet in navigation and gravity determination whereas the inwardly directed ciliary cells may possibly serve in acoustic communication. Another element worthy of mention within the hair cells are yellow granules (yg). These yg's originate from the whip-like portion of the ciliary cells that are distributed beneath the frons plate, and also in the yellow stripes of the gastral cuticle. Conceivably, these yellow granules, in both cases, may play a role in the absorption and storage of solar energy. In summary, ciliary structures are involved in the hornet in gravity sensing, in acoustical communication and in light sensing, i.e., with some similarity with what happens in vertebrates in the inner ear and in the photoreceptor.

Electrical properties of hornet silk: temperature dependence.

Hornet silk is a polymer of amino acids. One of the known properties of polymers is their electrical activity. The present study describes the results of electrical measurements carried out vertically on the silk cap of pupae of the Oriental hornet Vespa orientalis (Hymenoptera, Vespinae). The measurements undertaken were the temperature-dependent electric current, voltage and resistance, all measured within the range of biological temperatures, as well as the capacitance. The temperature-dependent spontaneous current attained values up to 327 nano Amperes (nA) while the maximal voltage reached 347 millivolt (mV). The electrical resistance was low and steady (1-20 mu omega) at temperatures ranging between 19-32 degrees C, but at lower or higher temperature it increased fairly sharply by about three orders of magnitude. The electrical capacitance, computed according to the discharge curve (decay curve) amounted to 0.4 microFarad (microF). The paper also discusses the role of the pupal silk as producer of a 'clean room' while the cuticle is being laid down by the pupae after undergoing metamorphosis, as well as the significance of the measured electrical parameters vis-à-vis the developing pupae.

Yellow pigment granules in hornets: their origin and development.

The present article discusses the yellow pigment in the cuticle of the Oriental hornet Vespa orientalis (Vespinae, Hymenoptera). This insect possesses, both in its gaster region and its head plates, yellow pigment granules that are located underneath the upper layers of the cuticle. All other regions of its body are endowed with a colour ranging from brown to black. As for the yellow granules, some occur within cells while others bud off from the cells inside tubular extensions that interpenetrate the cuticular layers and create accumulations of pigment. Whichever the case, the yellow granules invariably are approximately 0.5 microm in diameter and arranged in three longitudinally extending concentric cylinders, with the innermost cylinder comprising a string that interconnects between the various granules. Our paper discusses the physical properties of these yellow granules and their possible role in everyday hornet life.

Temperature distribution and electrical properties along the Oriental hornet body.

The hornet is an endothermic insect. Daily variations in hornet surface temperature were measured. Three peaks were found between 9:30 and 10: 30 a.m., 11 and 12 a.m. and between 2 and 3 p.m. Electrical current and voltage values were highest along the head. Electrical current along the gaster and the head flowed towards the thorax, i.e., from body parts with minimal temperature towards the body part with maximal temperature. Current and voltage values measured across the cuticle of the gaster were about 5nA and 100 mV, respectively, and these were of the same order of magnitude as the current and voltage values along the cuticle. It was found that: 1) temperature regulation most probably originates in the thorax and 2) there is a correlation between the temperature distribution along the hornet body surface and levels of the cuticular electrical signals.

Communication by electrical means in social insects.

Social insects, belonging to the order Hymenoptera, maintain a fixed, optimal temperature in their nest. Thus, in social wasps and hornets, the optimal nest temperature is 29 degrees C, despite the fact that they are distributed in regions of varying climates both in the northern and southern hemispheres of the globe. Since hornets and bees are relatively small insects, determination of their own body temperature as well as that of their nest and the brood was made via thermometers or by the use of infrared (IR) rays. It has been suggested that thermoregulation in social insect colonies is effected primarily by the adult insects via muscle activation, that is, fluttering of their wings, which can raise both their own and the ambient temperature by many degrees centigrade. However, the larval brood can also contribute to the thermoregulation by acting as heat resources and thereby raising the ambient temperature by 1-2 degrees C. To this end, the adult hornets are endowed with a well-developed musculature and their larvae, too, have muscles that enable them to move about. Not so the hornet pupae which are enclosed in a silk envelope (the cocoon), with a rather thick silk cap spun by the pupating larvae, and have rather undeveloped muscles. In the latter instance, it stands to reason that the pupae benefit from the nest warming achieved primarily by the adult hornets, but how is the information regarding their thermal needs relayed from them to the adults? Previously we showed that the adult hornets are attracted to the pupae by pheromones released by the latter, but such chemical compounds can only convey information of a general nature and we are still left with the question as to how the adult hornet can gauge or ascertain the temperature of a single insulated pupa. The present study provides evidence that the hornet pupa can indeed transmit information regarding its body temperature via electrical means.