ABSTRACT
A microphthalmic strain of mice was used to study immunoresponse of the retinoblastoma protein. Comparing wild-type, heterozygote and homozygote microphthalmic eyes, we found an increasing labelling of phosphorylated retinoblastoma protein (pRb) in the retinal pigment epithelium. Additionally, microphthalmic eyes expressed pRb in the neuroepithelium. Especially rosettes were strongly labelled.
Subject(s)
Microphthalmos/pathology , Retinal Neoplasms/pathology , Retinoblastoma Protein/immunology , Retinoblastoma/pathology , Animals , Disease Models, Animal , Humans , Mice , Microphthalmos/immunology , Retina/chemistry , Retinal Neoplasms/immunology , Retinoblastoma/immunology , Retinoblastoma Protein/biosynthesis , Retinoblastoma Protein/pharmacologyABSTRACT
A study about the distribution of neuronal nitric oxide synthase (n-NOS) and inducible NOS (i-NOS) was performed in microphthalmic retinas at postnatal day (pd) 2 and pd 7. Neither n-NOS nor i-NOS immunoresponse could be detected at pd 2. At pd 7, i-NOS activity was revealed in the retinas of mi/mi littermates but not in those of heterozygotes or wild-type littermates. The expression of n-NOS corresponded to the postnatal retinal development. Retinal rosettes were strongly labelled.
