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1.
Vet World ; 14(4): 835-840, 2021 Apr.
Article in English | MEDLINE | ID: mdl-34083928

ABSTRACT

BACKGROUND AND AIM: Immune-modulating molecules mainly act on innate immune cells, which are central to early defense against invading pathogens and contribute to developing adaptive immunity. Yeast-extracted ß-glucan, a model immune-modulating molecule, is widely used in several animal species; however, its effect on horse immune parameters has not been thoroughly investigated yet. This study aimed to evaluate the effects of orally administered ß-glucan on selected innate immune parameters in horses. MATERIALS AND METHODS: Eighteen thoroughbred horses were assigned equally into three groups as follows: One control group (no ß-glucan) and two ß-glucan experimental groups (one received 125 mg and the other 2 g of ß-glucan per day for 28 days). Blood samples were collected before and at the end of the experiment for hematological analysis, whole blood phagocytosis, respiratory burst assays, and to assess the serum lysozyme and complement hemolytic activities. RESULTS: At the end of the experiment, significant decreases (p<0.05) in monocyte numbers were observed in the control horses (258.8±45.9 vs. 115.3±41.5) and in those fed 125 mg/day of ß-glucan (208.8±72.3 vs. 99.2±60.7), whereas a significant increase in numbers was noted in the horses that were fed 2 g/day of ß-glucan (303.5±45.8 vs. 429.8±86.0; p<0.05). The natural hemolytic activity of the complement was higher only in horses fed 2 g/day of ß-glucan (p=0.018) compared to the other groups. The hemolytic activity in the classical pathway was higher in those fed 125 mg/day (p=0.0035) and 2 g/day of ß-glucan (p=0.0001). CONCLUSION: ß-glucan improves important innate immune parameters and might be fed to horses before stressful events.

2.
Braz J Microbiol ; 49 Suppl 1: 68-75, 2018 Nov.
Article in English | MEDLINE | ID: mdl-29866609

ABSTRACT

Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp® plate coated with 50ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736 samples) and beef cattle (451 samples) with unknown status to BLV. We found that 31.1% (229/736) and 9.5% (43/451) of samples amongst dairy and beef cattle, respectively, had IgGs to BLV. The rate of agreement with a commercial competitive ELISA was 84.3% with a κ value of 0.68. Thus, our BLVp24r iELISA is suitable to detect BLV infected animals and should be a useful tool to control BLV infection in cattle.


Subject(s)
Antibodies, Viral/blood , Capsid Proteins/immunology , Enzootic Bovine Leukosis/diagnosis , Enzyme-Linked Immunosorbent Assay/methods , Leukemia Virus, Bovine/immunology , Serologic Tests/methods , Animals , Capsid Proteins/analysis , Capsid Proteins/genetics , Cattle , Enzootic Bovine Leukosis/blood , Enzootic Bovine Leukosis/virology , Enzyme-Linked Immunosorbent Assay/instrumentation , Leukemia Virus, Bovine/genetics , Leukemia Virus, Bovine/isolation & purification , Recombinant Proteins/analysis , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Sensitivity and Specificity
3.
Braz. j. microbiol ; 49(supl.1): 68-75, 2018. tab, graf
Article in English | LILACS | ID: biblio-974318

ABSTRACT

Abstract Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp ® plate coated with 50 ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450 nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736 samples) and beef cattle (451 samples) with unknown status to BLV. We found that 31.1% (229/736) and 9.5% (43/451) of samples amongst dairy and beef cattle, respectively, had IgGs to BLV. The rate of agreement with a commercial competitive ELISA was 84.3% with a κ value of 0.68. Thus, our BLVp24r iELISA is suitable to detect BLV infected animals and should be a useful tool to control BLV infection in cattle.


Subject(s)
Animals , Cattle , Enzyme-Linked Immunosorbent Assay/methods , Serologic Tests/methods , Enzootic Bovine Leukosis/diagnosis , Leukemia Virus, Bovine/immunology , Capsid Proteins/immunology , Antibodies, Viral/blood , Recombinant Proteins/analysis , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Enzyme-Linked Immunosorbent Assay/instrumentation , Sensitivity and Specificity , Enzootic Bovine Leukosis/blood , Enzootic Bovine Leukosis/virology , Leukemia Virus, Bovine/isolation & purification , Leukemia Virus, Bovine/genetics , Capsid Proteins/analysis , Capsid Proteins/genetics
4.
Article in English | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469643

ABSTRACT

Abstract Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp ® plate coated with 50 ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450 nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736 samples) and beef cattle (451 samples) with unknown status to BLV. We found that 31.1% (229/736) and 9.5% (43/451) of samples amongst dairy and beef cattle, respectively, had IgGs to BLV. The rate of agreement with a commercial competitive ELISA was 84.3% with a value of 0.68. Thus, our BLVp24r iELISA is suitable to detect BLV infected animals and should be a useful tool to control BLV infection in cattle.

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