Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 35
Filter
1.
Article in Portuguese | VETINDEX | ID: biblio-1391325

ABSTRACT

O tumor ósseo multilobular, também conhecido como osteocondrossarcoma multilobular ou chondroma rodens, é um tumor maligno de crescimento lento, localmente invasivo, capaz de comprimir e invadir o tecido adjacente. Sua ocorrência é maior nos ossos planos do crânio e palato duro. Os sinais clínicos dependem da localização do tumor e, geralmente, estão relacionados à compressão de estruturas adjacentes. Neste relato, descreve-se um caso de tumor ósseo multilobular em uma cadela de 9 anos de idade, raça yorkshire terrier, com crescimento progressivo em região sobreposta à topografia de arco zigomático esquerdo e porção caudal do ramo mandibular esquerdo. Após realização de avaliação radiográfica de crânio, tomografia computadorizada e investigação laboratorial, foi realizada a remoção cirúrgica e exame histopatológico, que confirmaram a suspeita de tumor ósseo multilobular. Portanto, a avaliação histopatológica associada aos exames de imagem permitiu o estabelecimento do diagnóstico de tumor ósseo multilobular, uma neoplasia pouco descrita na clínica veterinária brasileira em cães de pequeno porte.(AU)


Multilobularbone tumor, also known as multilobular osteochondrosarcoma or chondroma rodens, it is a slow-growing, locally invasive, malignant tumor capable of compressing and invading adjacenttissue. Its occurrence is higher in the flat bones of the skull and hard palate. Clinical signs depend on the location of the tumor and are usually related to compression of adjacent structures. This report describes a case of multilobular bone tumor in a nine-year-old female yorkshire terrier breed, with progressive growth in a region overlapping the topography of the left zygomatic arch and the caudal portion of the left mandibular ramus. After performing a radiographic evaluation of the skull, computed tomography and laboratory investigation, surgical removal and histopathology were performed, which confirmed the suspicion of a multilobular bonetumor. Therefore, the histopathology associated with imaging exams allowed the establishment of a diagnosis of multilobular bonetumor, a neoplasm rarely described in the Brazilian veterinary clinic of small dogs.(AU)


Subject(s)
Animals , Dogs , Skull/anatomy & histology , Bone Neoplasms/diagnosis , Chondromatosis/diagnosis , Palate, Hard/anatomy & histology
2.
Acta Cir Bras ; 36(3): e360306, 2021.
Article in English | MEDLINE | ID: mdl-33978062

ABSTRACT

PURPOSE: To evaluate the preventive cardioprotective effects of resveratrol and grape products, such as grape juice and red wine, in animal model of cardiac ischemia and reperfusion. METHODS: Male Wistar rats orally pretreated for 21-days with resveratrol and grape products were anesthetized and placed on mechanical ventilation to surgically induce cardiac ischemia and reperfusion by obstruction (ischemia) followed by liberation (reperfusion) of blood circulation in left descending coronary artery. These rats were submitted to the electrocardiogram (ECG) analysis to evaluate the effects of pretreatment with resveratrol and grape products on the incidence of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) resulting from cardiac ischemia and reperfusion. RESULTS: It was observed that the incidence of AVB was significantly lower in rats pretreated with resveratrol (25%), grape juice (37.5%) or red wine (12.5%) than in rats treated with saline solution (80%) or ethanol (80%). Similarly, incidence of LET was also significantly lower in rats pretreated with resveratrol (25%), grape juice (25%) or red wine (0%) than in rats treated with saline solution (62.5%) or ethanol (75%). CONCLUSIONS: These results indicate that the cardioprotective response stimulated by resveratrol and grape products prevents the lethal cardiac arrhythmias in animal model of ischemia and reperfusion, supporting the idea that this treatment can be beneficial for prevention of severe cardiac arrhythmias in patients with ischemic heart disease.


Subject(s)
Stilbenes , Vitis , Animals , Arrhythmias, Cardiac/etiology , Arrhythmias, Cardiac/prevention & control , Humans , Ischemia , Male , Rats , Rats, Wistar , Reperfusion , Resveratrol/pharmacology , Stilbenes/pharmacology
3.
Acta cir. bras. ; 36(3): e360306, 2021. graf
Article in English | VETINDEX | ID: vti-30533

ABSTRACT

Purpose: To evaluate the preventive cardioprotective effects of resveratrol and grape products, such as grape juice and red wine, in animal model of cardiac ischemia and reperfusion. Methods: Male Wistar rats orally pretreated for 21-days with resveratrol and grape products were anesthetized and placed on mechanical ventilation to surgically induce cardiac ischemia and reperfusion by obstruction (ischemia) followed by liberation (reperfusion) of blood circulation in left descending coronary artery. These rats were submitted to the electrocardiogram (ECG) analysis to evaluate the effects of pretreatment with resveratrol and grape products on the incidence of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) resulting from cardiac ischemia and reperfusion. Results: It was observed that the incidence of AVB was significantly lower in rats pretreated with resveratrol (25%), grape juice (37.5%) or red wine (12.5%) than in rats treated with saline solution (80%) or ethanol (80%). Similarly, incidence of LET was also significantly lower in rats pretreated with resveratrol (25%), grape juice (25%) or red wine (0%) than in rats treated with saline solution (62.5%) or ethanol (75%). Conclusion: These results indicate that the cardioprotective response stimulated by resveratrol and grape products prevents the lethal cardiac arrhythmias in animal model of ischemia and reperfusion, supporting the idea that this treatment can be beneficial for prevention of severe cardiac arrhythmias in patients with ischemic heart disease.(AU)


Subject(s)
Animals , Rats , Cardiotonic Agents , Resveratrol/administration & dosage , Vitis , Arrhythmias, Cardiac/prevention & control , Myocardial Reperfusion/veterinary , Myocardial Ischemia/veterinary
4.
Acta cir. bras ; Acta cir. bras;36(3): e360306, 2021. graf
Article in English | LILACS | ID: biblio-1248539

ABSTRACT

ABSTRACT Purpose To evaluate the preventive cardioprotective effects of resveratrol and grape products, such as grape juice and red wine, in animal model of cardiac ischemia and reperfusion. Methods Male Wistar rats orally pretreated for 21-days with resveratrol and grape products were anesthetized and placed on mechanical ventilation to surgically induce cardiac ischemia and reperfusion by obstruction (ischemia) followed by liberation (reperfusion) of blood circulation in left descending coronary artery. These rats were submitted to the electrocardiogram (ECG) analysis to evaluate the effects of pretreatment with resveratrol and grape products on the incidence of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) resulting from cardiac ischemia and reperfusion. Results It was observed that the incidence of AVB was significantly lower in rats pretreated with resveratrol (25%), grape juice (37.5%) or red wine (12.5%) than in rats treated with saline solution (80%) or ethanol (80%). Similarly, incidence of LET was also significantly lower in rats pretreated with resveratrol (25%), grape juice (25%) or red wine (0%) than in rats treated with saline solution (62.5%) or ethanol (75%). Conclusions These results indicate that the cardioprotective response stimulated by resveratrol and grape products prevents the lethal cardiac arrhythmias in animal model of ischemia and reperfusion, supporting the idea that this treatment can be beneficial for prevention of severe cardiac arrhythmias in patients with ischemic heart disease.


Subject(s)
Humans , Animals , Male , Rats , Stilbenes/pharmacology , Vitis , Arrhythmias, Cardiac/etiology , Arrhythmias, Cardiac/prevention & control , Reperfusion , Rats, Wistar , Resveratrol/pharmacology , Ischemia
5.
Acta Cir Bras ; 35(3): e202000306, 2020 May 20.
Article in English | MEDLINE | ID: mdl-32692797

ABSTRACT

PURPOSE: To evaluate whether the attenuation of mitochondrial Ca2+ overload produced by pharmacological blockade of mitochondrial Ca2+ uniporter (MCU) protects the myocardium against injuries caused by cardiac ischemia and reperfusion (CIR). METHODS: CIR was induced in adult male Wistar rats (300-350 g) by occlusion of the left anterior descendent coronary artery (10 min), followed by reperfusion (120 min). Rats were treated with different doses of MCU blocker ruthenium red (RuR), administered 5 min before ischemia or reperfusion. RESULTS: In untreated rats, the incidences of ventricular arrhythmias (VA), atrioventricular block (AVB) and the lethality (LET) induced by CIR were 85%, 79% and 70%, respectively. In rats treated with RuR before ischemia, the incidences of VA, AVB and LET were significantly reduced to 62%, 25% and 25%, respectively. In rats treated with RuR after ischemia, the incidences of VA, AVB and LET were significantly reduced to 50%, 25% and 25%, respectively. CONCLUSION: The significant reduction of the incidence of CIR-induced VA, AVB and LET produced by the treatment with RuR indicates that the attenuation of mitochondrial Ca2+ overload produced by pharmacological blockade of MCU can protect the myocardium against injuries caused by CIR.


Subject(s)
Calcium Channels , Myocardial Ischemia , Myocardial Reperfusion Injury , Animals , Calcium , Calcium Channels/drug effects , Male , Myocardial Ischemia/drug therapy , Myocardial Reperfusion Injury/drug therapy , Rats , Rats, Wistar
6.
Acta cir. bras. ; 35(3): e202000306, May 20, 2020. graf
Article in English | VETINDEX | ID: vti-27760

ABSTRACT

Purpose To evaluate whether the attenuation of mitochondrial Ca2+ overload produced by pharmacological blockade of mitochondrial Ca2+ uniporter (MCU) protects the myocardium against injuries caused by cardiac ischemia and reperfusion (CIR). Methods CIR was induced in adult male Wistar rats (300-350 g) by occlusion of the left anterior descendent coronary artery (10 min), followed by reperfusion (120 min). Rats were treated with different doses of MCU blocker ruthenium red (RuR), administered 5 min before ischemia or reperfusion. Results In untreated rats, the incidences of ventricular arrhythmias (VA), atrioventricular block (AVB) and the lethality (LET) induced by CIR were 85%, 79% and 70%, respectively. In rats treated with RuR before ischemia, the incidences of VA, AVB and LET were significantly reduced to 62%, 25% and 25%, respectively. In rats treated with RuR after ischemia, the incidences of VA, AVB and LET were significantly reduced to 50%, 25% and 25%, respectively. Conclusion The significant reduction of the incidence of CIR-induced VA, AVB and LET produced by the treatment with RuR indicates that the attenuation of mitochondrial Ca2+ overload produced by pharmacological blockade of MCU can protect the myocardium against injuries caused by CIR.(AU)


Subject(s)
Animals , Rats , Rats, Wistar , Ischemia/therapy , Ischemia/veterinary , Reperfusion/veterinary , Cardiotonic Agents , Myocardial Contusions/prevention & control , Myocardial Contusions/veterinary , Calcium
8.
R. Educ. contin. Med. Vet. Zoot. ; 18(2): [e38068], set. 2020. ilus
Article in Portuguese | VETINDEX | ID: vti-28762

ABSTRACT

A dermatomiosite canina é uma vasculopatia inflamatória da pele e músculos, com manifestações cutâneas envolvendo a face, orelhas, extremidades da cauda e distais por sobre proeminências ósseas. O envolvimento muscular manifesta-se sob a forma de mioatrofia cefálica, dificuldade de deglutição, redução do reflexo do vômito e marcha atípica. A dermatomiosite canina é classificada em dermatomiosite canina familiar e em forma variante ou dermatomiosite-símile. Neste relato descreve-se um caso de dermatomiosite-símile em cadela de dois anos de idade, sem raça definida, que apresentava necrose isquêmica dos pavilhões auriculares e atrofia dos músculos temporal e masseter. O exame dermo-histopatológico realizado em fragmentos de pele dos pavilhões auriculares, região cefálica e amostras de tecido muscular (temporal e masseter) confirmou a existência de dermatite perivascular e foliculite de interface pobre em células, focos de atrofia folicular e de fibras musculares e moderada fibrose dérmica. Portanto a histopatologia, associada às manifestações clínicas do animal, permitiu o estabelecimento do diagnóstico de dermatomiosite-símile, enfermidade pouco descrita na dermatologia veterinária brasileira.(AU)


Canine dermatomyositis is a skin and muscles inflammatory vasculopathy with cuta-neous manifestations involving face, ears, tail and distal ends over bony prominences. Mus-cle involvement manifests itself in the form of muscles of the head, difficulty in swallowing, reduction of reflex of vomit and atypical gait. Canine dermatomyositis is classified into variant form or dermatomyositis-like. This report describes a case of dermatomyositis-like in a two-year-old femalemixed-breed dog that presented ischemic necrosis of pinna and temporal and masseter muscle atrophy. In histopathological examinations performed with skin fragments of pinna, cephalic region and muscle samples (temporal and masseter), it was detected a perivascular dermatitis and folliculitis with poor cell interface, foci of follicular atrophy and muscle fibers with moderate dermal fibrosis confirming the diagnosis of derma-tomyositis-like, relatively little described in brazilian veterinary dermatology.(AU)


Subject(s)
Animals , Dogs , Dogs/abnormalities , Dermatomyositis/classification , Dermatomyositis/diagnosis , Dermatomyositis/veterinary
9.
Acta cir. bras ; Acta cir. bras;35(3): e202000306, 2020. graf
Article in English | LILACS | ID: biblio-1130620

ABSTRACT

Abstract Purpose To evaluate whether the attenuation of mitochondrial Ca2+ overload produced by pharmacological blockade of mitochondrial Ca2+ uniporter (MCU) protects the myocardium against injuries caused by cardiac ischemia and reperfusion (CIR). Methods CIR was induced in adult male Wistar rats (300-350 g) by occlusion of the left anterior descendent coronary artery (10 min), followed by reperfusion (120 min). Rats were treated with different doses of MCU blocker ruthenium red (RuR), administered 5 min before ischemia or reperfusion. Results In untreated rats, the incidences of ventricular arrhythmias (VA), atrioventricular block (AVB) and the lethality (LET) induced by CIR were 85%, 79% and 70%, respectively. In rats treated with RuR before ischemia, the incidences of VA, AVB and LET were significantly reduced to 62%, 25% and 25%, respectively. In rats treated with RuR after ischemia, the incidences of VA, AVB and LET were significantly reduced to 50%, 25% and 25%, respectively. Conclusion The significant reduction of the incidence of CIR-induced VA, AVB and LET produced by the treatment with RuR indicates that the attenuation of mitochondrial Ca2+ overload produced by pharmacological blockade of MCU can protect the myocardium against injuries caused by CIR.


Subject(s)
Animals , Male , Rats , Calcium Channels/drug effects , Myocardial Reperfusion Injury/drug therapy , Myocardial Ischemia/drug therapy , Calcium , Rats, Wistar
11.
Article in Portuguese | VETINDEX | ID: biblio-1489060

ABSTRACT

A dermatomiosite canina é uma vasculopatia inflamatória da pele e músculos, com manifestações cutâneas envolvendo a face, orelhas, extremidades da cauda e distais por sobre proeminências ósseas. O envolvimento muscular manifesta-se sob a forma de mioatrofia cefálica, dificuldade de deglutição, redução do reflexo do vômito e marcha atípica. A dermatomiosite canina é classificada em dermatomiosite canina familiar e em forma variante ou dermatomiosite-símile. Neste relato descreve-se um caso de dermatomiosite-símile em cadela de dois anos de idade, sem raça definida, que apresentava necrose isquêmica dos pavilhões auriculares e atrofia dos músculos temporal e masseter. O exame dermo-histopatológico realizado em fragmentos de pele dos pavilhões auriculares, região cefálica e amostras de tecido muscular (temporal e masseter) confirmou a existência de dermatite perivascular e foliculite de interface pobre em células, focos de atrofia folicular e de fibras musculares e moderada fibrose dérmica. Portanto a histopatologia, associada às manifestações clínicas do animal, permitiu o estabelecimento do diagnóstico de dermatomiosite-símile, enfermidade pouco descrita na dermatologia veterinária brasileira.


Canine dermatomyositis is a skin and muscles inflammatory vasculopathy with cuta-neous manifestations involving face, ears, tail and distal ends over bony prominences. Mus-cle involvement manifests itself in the form of muscles of the head, difficulty in swallowing, reduction of reflex of vomit and atypical gait. Canine dermatomyositis is classified into variant form or dermatomyositis-like. This report describes a case of dermatomyositis-like in a two-year-old femalemixed-breed dog that presented ischemic necrosis of pinna and temporal and masseter muscle atrophy. In histopathological examinations performed with skin fragments of pinna, cephalic region and muscle samples (temporal and masseter), it was detected a perivascular dermatitis and folliculitis with poor cell interface, foci of follicular atrophy and muscle fibers with moderate dermal fibrosis confirming the diagnosis of derma-tomyositis-like, relatively little described in brazilian veterinary dermatology.


Subject(s)
Animals , Dogs , Dogs/abnormalities , Dermatomyositis/classification , Dermatomyositis/diagnosis , Dermatomyositis/veterinary
12.
Int J Mol Sci ; 20(16)2019 Aug 16.
Article in English | MEDLINE | ID: mdl-31426434

ABSTRACT

Ischemic heart diseases (IHD) are the leading cause of death worldwide. Although the principal form of treatment of IHD is myocardial reperfusion, the recovery of coronary blood flow after ischemia can cause severe and fatal cardiac dysfunctions, mainly due to the abrupt entry of oxygen and ionic deregulation in cardiac cells. The ability of these cells to protect themselves against injury including ischemia and reperfusion (I/R), has been termed "cardioprotection". This protective response can be stimulated by pharmacological agents (adenosine, catecholamines and others) and non-pharmacological procedures (conditioning, hypoxia and others). Several intracellular signaling pathways mediated by chemical messengers (enzymes, protein kinases, transcription factors and others) and cytoplasmic organelles (mitochondria, sarcoplasmic reticulum, nucleus and sarcolemma) are involved in cardioprotective responses. Therefore, advancement in understanding the cellular and molecular mechanisms involved in the cardioprotective response can lead to the development of new pharmacological and non-pharmacological strategies for cardioprotection, thus contributing to increasing the efficacy of IHD treatment. In this work, we analyze the recent advances in pharmacological and non-pharmacological strategies of cardioprotection.


Subject(s)
Cardiotonic Agents/therapeutic use , Ischemic Preconditioning, Myocardial/methods , Myocardial Ischemia/therapy , Animals , Humans , Myocardial Ischemia/pathology , Myocardial Reperfusion Injury/pathology , Myocardial Reperfusion Injury/therapy , Myocardium/pathology
13.
Acta Cir Bras ; 34(5): e201900505, 2019.
Article in English | MEDLINE | ID: mdl-31166461

ABSTRACT

PURPOSE: To evaluate the cardioprotective response of the pharmacological modulation of ß-adrenergic receptors (ß-AR) in animal model of cardiac ischemia and reperfusion (CIR), in spontaneously hypertensive (SHR) and normotensive (NWR) rats. METHODS: CIR was induced by the occlusion of left anterior descendent coronary artery (10 min) and reperfusion (75 min). The SHR was treated with ß-AR antagonist atenolol (AT, 10 mg/kg, IV) 5 min before CIR, and NWR were treated with ß-AR agonist isoproterenol (ISO, 0.5 mg/kg, IV) 5 min before CIR. RESULTS: The treatment with AT increased the incidence of VA, AVB and LET in SHR, suggesting that spontaneous cardioprotection in hypertensive animals was abolished by blockade of ß-AR. In contrast, the treatment with ISO significantly reduced the incidence of ventricular arrhythmia, atrioventricular blockade and lethality in NWR (30%, 20% and 20%, respectively), suggesting that the activation of ß-AR stimulate cardioprotection in normotensive animals. Serum CK-MB were higher in SHR/CIR and NWR/CIR compared to respective SHAM group (not altered by treatment with AT or ISO). CONCLUSION: The pharmacological modulation of ß-AR could be a new cardioprotective strategy for the therapy of myocardial dysfunctions induced by CIR related to cardiac surgery and cardiovascular diseases.


Subject(s)
Adrenergic beta-1 Receptor Antagonists/pharmacology , Adrenergic beta-Agonists/pharmacology , Atenolol/pharmacology , Cardiotonic Agents/pharmacology , Isoproterenol/pharmacology , Myocardial Reperfusion Injury/drug therapy , Receptors, Adrenergic, beta/drug effects , Animals , Biomarkers/blood , Blood Pressure/drug effects , Creatine Kinase, MB Form/blood , Heart Function Tests , Male , Myocardial Reperfusion Injury/blood , Myocardial Reperfusion Injury/physiopathology , Rats, Inbred SHR , Reproducibility of Results , Time Factors , Treatment Outcome
14.
Acta cir. bras. ; 34(5): e201900505, June 3, 2019. graf
Article in English | VETINDEX | ID: vti-23226

ABSTRACT

Purpose: To evaluate the cardioprotective response of the pharmacological modulation of β-adrenergic receptors (β-AR) in animal model of cardiac ischemia and reperfusion (CIR), in spontaneously hypertensive (SHR) and normotensive (NWR) rats. Methods: CIR was induced by the occlusion of left anterior descendent coronary artery (10 min) and reperfusion (75 min). The SHR was treated with β-AR antagonist atenolol (AT, 10 mg/kg, IV) 5 min before CIR, and NWR were treated with β-AR agonist isoproterenol (ISO, 0.5 mg/kg, IV) 5 min before CIR. Results: The treatment with AT increased the incidence of VA, AVB and LET in SHR, suggesting that spontaneous cardioprotection in hypertensive animals was abolished by blockade of β-AR. In contrast, the treatment with ISO significantly reduced the incidence of ventricular arrhythmia, atrioventricular blockade and lethality in NWR (30%, 20% and 20%, respectively), suggesting that the activation of β-AR stimulate cardioprotection in normotensive animals. Serum CK-MB were higher in SHR/CIR and NWR/CIR compared to respective SHAM group (not altered by treatment with AT or ISO). Conclusion: The pharmacological modulation of β-AR could be a new cardioprotective strategy for the therapy of myocardial dysfunctions induced by CIR related to cardiac surgery and cardiovascular diseases.(AU)


Subject(s)
Animals , Rats , Cardiovascular Diseases/drug therapy , Cardiovascular Diseases/prevention & control , Receptors, Adrenergic, beta/analysis , Receptors, Adrenergic, beta/drug effects , Reperfusion , Myocardial Ischemia , Atenolol/therapeutic use , Isoproterenol/therapeutic use , Disease Models, Animal
15.
Acta cir. bras ; Acta cir. bras;34(5): e201900505, 2019. graf
Article in English | LILACS | ID: biblio-1010872

ABSTRACT

Abstract Purpose: To evaluate the cardioprotective response of the pharmacological modulation of β-adrenergic receptors (β-AR) in animal model of cardiac ischemia and reperfusion (CIR), in spontaneously hypertensive (SHR) and normotensive (NWR) rats. Methods: CIR was induced by the occlusion of left anterior descendent coronary artery (10 min) and reperfusion (75 min). The SHR was treated with β-AR antagonist atenolol (AT, 10 mg/kg, IV) 5 min before CIR, and NWR were treated with β-AR agonist isoproterenol (ISO, 0.5 mg/kg, IV) 5 min before CIR. Results: The treatment with AT increased the incidence of VA, AVB and LET in SHR, suggesting that spontaneous cardioprotection in hypertensive animals was abolished by blockade of β-AR. In contrast, the treatment with ISO significantly reduced the incidence of ventricular arrhythmia, atrioventricular blockade and lethality in NWR (30%, 20% and 20%, respectively), suggesting that the activation of β-AR stimulate cardioprotection in normotensive animals. Serum CK-MB were higher in SHR/CIR and NWR/CIR compared to respective SHAM group (not altered by treatment with AT or ISO). Conclusion: The pharmacological modulation of β-AR could be a new cardioprotective strategy for the therapy of myocardial dysfunctions induced by CIR related to cardiac surgery and cardiovascular diseases.


Subject(s)
Animals , Male , Atenolol/pharmacology , Cardiotonic Agents/pharmacology , Myocardial Reperfusion Injury/drug therapy , Receptors, Adrenergic, beta/drug effects , Adrenergic beta-Agonists/pharmacology , Adrenergic beta-1 Receptor Antagonists/pharmacology , Isoproterenol/pharmacology , Rats, Inbred SHR , Time Factors , Blood Pressure/drug effects , Biomarkers/blood , Myocardial Reperfusion Injury/physiopathology , Myocardial Reperfusion Injury/blood , Reproducibility of Results , Treatment Outcome , Creatine Kinase, MB Form/blood , Heart Function Tests
16.
Acta Cir Bras ; 33(7): 588-596, 2018 Jul.
Article in English | MEDLINE | ID: mdl-30110060

ABSTRACT

PURPOSE: To investigate the cardioprotective effects of ischemic preconditioning (preIC) and postconditioning (postIC) in animal model of cardiac ischemia/reperfusion. METHODS: Adult rats were submitted to protocol of cardiac ischemia/reperfusion (I/R) and randomized into three experimental groups: cardiac I/R (n=33), preCI + cardiac I/R (n=7) and postCI + cardiac I/R (n=8). After this I/R protocol, the incidence of ventricular arrhythmia (VA), atrioventricular block (AVB) and lethality (LET) was evaluated using the electrocardiogram (ECG) analysis. RESULTS: After reestablishment of coronary blood flow, we observed variations of the ECG trace with increased incidence of ventricular arrhythmia (VA) (85%), atrioventricular block (AVB) (79%), and increase of lethality (70%) in cardiac I/R group. The comparison between I/R + preIC group with I/R group demonstrated significant reduction in VA incidence to 28%, AVB to 0% and lethality to 14%. The comparison of I/R + postIC group with I/R group was observed significance reduction in AVB incidence to 25% and lethality to 25%. CONCLUSION: The preconditioning strategies produce cardioprotection more efficient that postconditioning against myocardial dysfunctions and lethality by cardiac ischemia and reperfusion.


Subject(s)
Ischemic Postconditioning/methods , Ischemic Preconditioning, Myocardial/methods , Myocardial Reperfusion Injury/prevention & control , Animals , Arrhythmias, Cardiac/physiopathology , Arrhythmias, Cardiac/prevention & control , Atrioventricular Block/physiopathology , Atrioventricular Block/prevention & control , Electrocardiography , Male , Myocardial Reperfusion Injury/physiopathology , Random Allocation , Rats, Wistar , Reproducibility of Results , Time Factors , Treatment Outcome
17.
Acta Cir Bras ; 33(6): 524-532, 2018 06.
Article in English | MEDLINE | ID: mdl-30020314

ABSTRACT

PURPOSE: To evaluate in vivo animal model of cardiac ischemia/reperfusion the cardioprotective activity of pancreatic lipase inhibitor of the orlistat. METHODS: Adult male Wistar rats were anesthetized, placed on mechanical ventilation and underwent surgery to induce cardiac I/R by obstructing left descending coronary artery followed by reperfusion to evaluation of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) with pancreatic lipase inhibitor orlistat (ORL). At the end of reperfusion, blood samples were collected for determination of triglycerides (TG), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), high-density lipoprotein (HDL), lactate dehydrogenase (LDH), creatine kinase (CK), and creatine kinase-MB (CK-MB). RESULTS: Treatment with ORL has been able to decrease the incidence of VA, AVB and LET. Besides that, treatment with ORL reduced serum concentrations of CK and LDL, but did not alter the levels of serum concentration of TG, VLDL and HDL. CONCLUSION: The reduction of ventricular arrhythmias, atrioventricular block, and lethality and serum levels of creatine kinase produced by treatment with orlistat in animal model of cardiac isquemia/reperfusion injury suggest that ORL could be used as an efficient cardioprotective therapeutic strategy to attenuate myocardial damage related to acute myocardial infarction.


Subject(s)
Cardiotonic Agents/pharmacology , Lactones/pharmacology , Myocardial Infarction/prevention & control , Myocardial Reperfusion Injury/prevention & control , Animals , Arrhythmias, Cardiac/prevention & control , Atrioventricular Block/prevention & control , Creatine Kinase/blood , Electrocardiography , L-Lactate Dehydrogenase/blood , Lipoproteins, HDL/blood , Lipoproteins, LDL/blood , Lipoproteins, VLDL/blood , Male , Myocardial Infarction/blood , Myocardial Reperfusion Injury/blood , Orlistat , Random Allocation , Rats, Wistar , Reproducibility of Results , Risk Factors , Treatment Outcome , Triglycerides/blood
18.
Acta cir. bras. ; 33(7): 588-596, jul. 2018. graf
Article in English | VETINDEX | ID: vti-18277

ABSTRACT

Purpose: To investigate the cardioprotective effects of ischemic preconditioning (preIC) and postconditioning (postIC) in animal model of cardiac ischemia/reperfusion. Methods: Adult rats were submitted to protocol of cardiac ischemia/reperfusion (I/R) and randomized into three experimental groups: cardiac I/R (n=33), preCI + cardiac I/R (n=7) and postCI + cardiac I/R (n=8). After this I/R protocol, the incidence of ventricular arrhythmia (VA), atrioventricular block (AVB) and lethality (LET) was evaluated using the electrocardiogram (ECG) analysis. Results: After reestablishment of coronary blood flow, we observed variations of the ECG trace with increased incidence of ventricular arrhythmia (VA) (85%), atrioventricular block (AVB) (79%), and increase of lethality (70%) in cardiac I/R group. The comparison between I/R + preIC group with I/R group demonstrated significant reduction in VA incidence to 28%, AVB to 0% and lethality to 14%. The comparison of I/R + postIC group with I/R group was observed significance reduction in AVB incidence to 25% and lethality to 25%. Conclusion: The preconditioning strategies produce cardioprotection more efficient that postconditioning against myocardial dysfunctions and lethality by cardiac ischemia and reperfusion.(AU)


Subject(s)
Animals , Male , Adult , Rats , Ischemic Postconditioning , Ischemic Preconditioning , Myocardial Reperfusion Injury/therapy , Cardiotonic Agents , Disease Models, Animal , Rats, Wistar
19.
Acta cir. bras ; Acta cir. bras;33(7): 588-596, July 2018. graf
Article in English | LILACS | ID: biblio-949370

ABSTRACT

Abstract Purpose: To investigate the cardioprotective effects of ischemic preconditioning (preIC) and postconditioning (postIC) in animal model of cardiac ischemia/reperfusion. Methods: Adult rats were submitted to protocol of cardiac ischemia/reperfusion (I/R) and randomized into three experimental groups: cardiac I/R (n=33), preCI + cardiac I/R (n=7) and postCI + cardiac I/R (n=8). After this I/R protocol, the incidence of ventricular arrhythmia (VA), atrioventricular block (AVB) and lethality (LET) was evaluated using the electrocardiogram (ECG) analysis. Results: After reestablishment of coronary blood flow, we observed variations of the ECG trace with increased incidence of ventricular arrhythmia (VA) (85%), atrioventricular block (AVB) (79%), and increase of lethality (70%) in cardiac I/R group. The comparison between I/R + preIC group with I/R group demonstrated significant reduction in VA incidence to 28%, AVB to 0% and lethality to 14%. The comparison of I/R + postIC group with I/R group was observed significance reduction in AVB incidence to 25% and lethality to 25%. Conclusion: The preconditioning strategies produce cardioprotection more efficient that postconditioning against myocardial dysfunctions and lethality by cardiac ischemia and reperfusion.


Subject(s)
Animals , Male , Myocardial Reperfusion Injury/prevention & control , Ischemic Preconditioning, Myocardial/methods , Ischemic Postconditioning/methods , Arrhythmias, Cardiac/physiopathology , Arrhythmias, Cardiac/prevention & control , Time Factors , Myocardial Reperfusion Injury/physiopathology , Random Allocation , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Electrocardiography , Atrioventricular Block/physiopathology , Atrioventricular Block/prevention & control
20.
Acta cir. bras. ; 33(6): 524-532, jun. 2018. graf
Article in English | VETINDEX | ID: vti-734725

ABSTRACT

Purpose: To evaluate in vivo animal model of cardiac ischemia/reperfusion the cardioprotective activity of pancreatic lipase inhibitor of the orlistat. Methods: Adult male Wistar rats were anesthetized, placed on mechanical ventilation and underwent surgery to induce cardiac I/R by obstructing left descending coronary artery followed by reperfusion to evaluation of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) with pancreatic lipase inhibitor orlistat (ORL). At the end of reperfusion, blood samples were collected for determination of triglycerides (TG), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), high-density lipoprotein (HDL), lactate dehydrogenase (LDH), creatine kinase (CK), and creatine kinase-MB (CK-MB). Results: Treatment with ORL has been able to decrease the incidence of VA, AVB and LET. Besides that, treatment with ORL reduced serum concentrations of CK and LDL, but did not alter the levels of serum concentration of TG, VLDL and HDL. Conclusion: The reduction of ventricular arrhythmias, atrioventricular block, and lethality and serum levels of creatine kinase produced by treatment with orlistat in animal model of cardiac isquemia/reperfusion injury suggest that ORL could be used as an efficient cardioprotective therapeutic strategy to attenuate myocardial damage related to acute myocardial infarction.(AU)


Subject(s)
Animals , Male , Adult , Rats , Myocardial Infarction/complications , Myocardial Reperfusion Injury/chemically induced , Cardiotonic Agents/analysis , Hypolipidemic Agents/pharmacology , Hypolipidemic Agents/therapeutic use , Biomarkers/analysis
SELECTION OF CITATIONS
SEARCH DETAIL