ABSTRACT
The experience of surgical treatment of double deviation of main vessels from right ventricle of transpositional type for the 1996-2001 period. Double ventricular correction was done in 22 (73.3%), singleventricular one--in 8 (26.7%) patients. Total hospital mortality was 18.1%. Tactics of surgical treatment of the failure, depending on anatomical peculiarities and the presence of concomitant failures, was determined. In the late follow-up period 22 (84.6%) patients, of that survived, were examined. The patients were followed up from 6 to 48 months, (24.4 +/- 12.3) months at average. The condition of patients was good. The meticulous estimation of the anatomy peculiarities before the operation permits to apply optimal surgical intervention and to improve result of correction.
Subject(s)
Cardiac Surgical Procedures/methods , Transposition of Great Vessels/complications , Transposition of Great Vessels/surgery , Ventricular Dysfunction, Right/etiology , Ventricular Dysfunction, Right/physiopathology , Adolescent , Child , Child, Preschool , Coronary Angiography , Echocardiography , Female , Humans , Infant , Infant, Newborn , Male , Preoperative Care , Retrospective Studies , Transposition of Great Vessels/diagnosis , Ventricular Dysfunction, Right/diagnosisABSTRACT
Commercial system of multimicrotests for biochemical identification of enterobacteria MMT E2 and routine tube tests were compared in a controlled epidemiologic experiment with 205 enterobacterial strains. The system has been developed at the Allergen Research and Production Amalgamation. System MMT E2 fairly well met the requirements to diagnostic preparations of this kind; its results were compatible and well reproducible. This system in complex with commercial MMT E1 system may be used for species identification of enterobacteria. Practical application of both the systems will considerably simplify the process of identification of these microorganisms.
Subject(s)
Bacteriological Techniques , Enterobacteriaceae Infections/diagnosis , Enterobacteriaceae/isolation & purification , Citrobacter/isolation & purification , Diagnosis, Differential , Enterobacter/isolation & purification , Enterobacteriaceae Infections/prevention & control , Escherichia/isolation & purification , Evaluation Studies as Topic , Klebsiella/isolation & purification , Proteus/isolation & purification , Providencia/isolation & purification , Salmonella/isolation & purification , Shigella/isolation & purification , Yersinia/isolation & purificationABSTRACT
Comparative evaluation of a system of multimicrotests for biochemical identification of enterobacteria (MMT E1) developed at the ALLERGEN Research and Production Amalgamation and of tube tests for the same purpose in a controlled epidemiologic experiment has shown that the suggested system meets the requirements to such diagnostic preparations by all the major qualitative parameters (compatibility and reproducibility of the test results). Practical use of MMT E1 system will simplify and facilitate the process of enterobacteria identification.
Subject(s)
Bacteriological Techniques , Enterobacteriaceae/isolation & purification , Enterobacteriaceae Infections/diagnosis , Enterobacteriaceae Infections/epidemiology , Evaluation Studies as TopicABSTRACT
The first Soviet kits for the serological identification of streptococci, groups A, B, and C, on the basis of the coagglutination test were developed. Each kit was intended for 35-40 determinations. The optimum concentration of streptococci during their identification by means of the reagents making up the kit was about 1.6 x 10(9) cells/ml. The specificity of the reagents in comparison with the results of the identification of streptococci by reference methods was 97.3 +/- 0.9%. The reagents making up the kits can be presumably used for solving a number of practical problems in the epidemiological surveillance of streptococcal infection.
Subject(s)
Serotyping/instrumentation , Streptococcus agalactiae/classification , Streptococcus pyogenes/classification , Streptococcus/classification , Agglutination Tests/instrumentation , Agglutination Tests/standards , Evaluation Studies as Topic , Humans , Latex Fixation Tests , Precipitin Tests , Reagent Kits, Diagnostic/standards , Reference Standards , Russia , Sensitivity and Specificity , Serotyping/standards , Streptococcal Infections/diagnosisABSTRACT
The data on the approbation of the diagnostic value of the enzyme immunoassay (EIA) system for the determination of diphtheria toxin in the blood sera of diphtheria patients and persons suspected for diphtheria are presented. The EIA system was prepared on the basis of F(ab)2 fractions of purified antidiphtheria antibodies. 240 serum samples from diphtheria and tonsillitis patients and from healthy persons were studied. Diphtheria toxin was determined in all patients with the toxic form of diphtheria and in 41.3% of patients with its localized forms. Blood was taken mainly of the first week of the disease. In healthy persons the results of EIA were negative. Thus, the trial of the assay system in a clinical laboratory showed its good diagnostic effectiveness. The use of this EIA system in medical practice is believed to be quite promising.
Subject(s)
Antigens, Bacterial/blood , Diphtheria Toxin/immunology , Diphtheria/diagnosis , Reagent Kits, Diagnostic , Adolescent , Adult , Blood Donors , Carrier State/diagnosis , Evaluation Studies as Topic , Female , Humans , Immunoenzyme Techniques/instrumentation , Male , Middle Aged , USSRABSTRACT
Klebsiella pneumoniae, serovar K1 strains, differ essentially in the composition of cellular fatty acids from serovar strains K 2, K 3, K 7-K 72, K 74-K 80 of this species and are analogous to K. rhinoscleromatis and K. ozaenae by the given trait. The differences concern the concentration of cyclopropane fatty acids and their biosynthetic precursors--monounsaturated fatty acids, that is typical of a number of other bacteria belonging to the related species and, evidently, may be a marker of phenetic differences at the level of related taxonomic groups. The data obtained evidence for taxonomic separation of K. pneumoniae serovar K1 strains from representatives of other serovars of the given species.
Subject(s)
Fatty Acids/analysis , Klebsiella pneumoniae/analysis , Klebsiella pneumoniae/classification , Phenotype , SerotypingABSTRACT
Cultures isolated from human and animal blood have been examined in tests with 3% KOH solution; their sensitivities to various concentrations of sulfanol have been tested in order to specify the tinctorial characteristics of these cultures; antibiotic sensitivity of this group of microorganisms has been studied with the use of discs. The findings evidence that gram-variable nonspore-carrying aerobic bacilli isolated from human and animal blood should be referred to gram-positive microorganisms; their antibiotic sensitivity has been detected. The tests employed in the study may be rationally used for the identification of polychromic bacteria isolated from the blood.
Subject(s)
Bacteria/isolation & purification , Blood/microbiology , Staining and Labeling , Animals , Drug Resistance, Microbial , HumansABSTRACT
Differential diagnosis of enterobacteria is based on the determination of beta-galactosidase enzyme, hydrolyzing lactose in the nutrient substrates; however, the tests suggested for its determination are time consuming and their use in practice is limited. Dry nutrient medium prepared by the authors containing o-nitrophenyl-betaD-pyranoside was tested on 1625 strains of enterobacteria in comparison with the Le Minor's ONPS test used at present. The results of beta-galactosidase determination by the, mentioned methods proved to coincide (significance level greater than 95%); this permits to recommend the method described--in dry nutrient medium--as a differential-diagnostic test for the identification of enterobacteria.
