Influence of different surface treatments and universal adhesives on the repair of CAD-CAM composite resins: An in vitro study.

STATEMENT OF PROBLEM: The repairability of computer-aided design and computer-aided manufacturing (CAD-CAM) composite resins might be adversely affected by the high degree of matrix polymerization that occurs during their manufacturing process. However, information on their repairability is lacking. PURPOSE: The purpose of this in vitro study was to evaluate the microtensile bond strength of CAD-CAM composite resins subjected to simulated repair procedures by using varying surface treatments and universal adhesives. MATERIAL AND METHODS: Four different CAD-CAM blocks (Brilliant Crios, Lava Ultimate, Shofu Block HC, and Vita Enamic) were thermocycled (5000 times, 5/55 °C) and divided into 4 groups according to the surface treatment: control, 9% hydrofluoric acid etching, aluminum oxide airborne-particle abrasion, and tribochemical silica airborne-particle abrasion. After surface treatments, the surface roughness was measured with a nanoindenter and further examined with scanning electron microscopy. After the application of 3 different universal adhesives (Clearfil Universal Bond, Prime&Bond Universal, and Single Bond Universal), the specimens were subjected to a simulated repair process with composite resin. Bonded specimens were cut into 1 mm2 beams, and microtensile bond strength values were determined until failure at a crosshead speed of 0.5 mm/min. The bond strength data were analyzed with 3-way analysis of variance, and surface roughness data were analyzed with 2-way analysis of variance tests. Pairwise analyses were performed with the Tukey test (α=.05). RESULTS: All surface treatments effectively improved repair microtensile bond strength values compared with the control (P<.05). Aluminum oxide airborne-particle abrasion had similar mean bond strength values compared with tribochemical silica airborne-particle abrasion (P>.05). Among the CAD-CAM blocks treated with hydrofluoric acid etching, Vita Enamic had the highest mean bond strength values. The highest mean microtensile bond strength repair values with the highest cohesive failure rates were found with the silane-containing universal adhesive (Single Bond Universal). CONCLUSIONS: Surface treatment with aluminum oxide airborne-particle abrasion and tribochemical silica airborne-particle abrasion produced successful repair results for aged resin nanoceramics, whereas hydrofluoric acid etching can be used for aged hybrid ceramic repair. Silane-containing universal adhesive reported increased bond strength. Application of universal adhesive after surface treatment is recommended to increase repair strength.

The efficacy of sustained-release chitosan microspheres containing recombinant human parathyroid hormone on MRONJ.

Treatment of patients with bisphosphonate usage is a significant concern for oral surgeons because it interferes with jaw bone turnover and regeneration. In case of adverse effects manifesting related to bisphosphonate use, oral surgeons are usually treating and keep the patient's symptoms under control. In this study, we aimed to investigate a new treatment protocol for medication-related osteonecrosis of the jaw (MRONJ). This treatment protocol consisted of administering human parathyroid hormone (hPTH) loaded chitosan microspheres which were prepared by ionotropic gelation method or/and the prepared microspheres were suspended in a poloxamer gel. After in-vitro optimization studies, the efficacy of the chosen formulations was evaluated in-vivo studies. Zoledronic acid was administered daily to forty-eight adult female Sprague-Dawley rats, divided into four experimental groups, at a daily concentration of 0.11 mg/kg over three weeks to induce the MRONJ model. At the end of this period, maxillary left molar teeth were extracted. In the first group, the subjects received no treatment. In the negative control group, poloxamer hydrogel containing empty microspheres were immediately applied to the soft tissues surrounding the extraction socket. The treatment group-1 was treated with local injections of poloxamer hydrogel containing hPTH. The treatment group-2 was treated with a single local injection of poloxamer hydrogel containing hPTH-loaded chitosan microspheres. Both treatment groups received a total of 7 µg of hPTH at the end of the treatment protocol. Our study demonstrates successful attenuation of MRONJ through a local drug delivery system combined with hPTH, as opposed to previously attempted treatment strategies.

The efficacy of sustained-release chitosan microspheres containing recombinant human parathyroid hormone on MRONJ

Abstract Treatment of patients with bisphosphonate usage is a significant concern for oral surgeons because it interferes with jaw bone turnover and regeneration. In case of adverse effects manifesting related to bisphosphonate use, oral surgeons are usually treating and keep the patient's symptoms under control. In this study, we aimed to investigate a new treatment protocol for medication-related osteonecrosis of the jaw (MRONJ). This treatment protocol consisted of administering human parathyroid hormone (hPTH) loaded chitosan microspheres which were prepared by ionotropic gelation method or/and the prepared microspheres were suspended in a poloxamer gel. After in-vitro optimization studies, the efficacy of the chosen formulations was evaluated in-vivo studies. Zoledronic acid was administered daily to forty-eight adult female Sprague-Dawley rats, divided into four experimental groups, at a daily concentration of 0.11 mg/kg over three weeks to induce the MRONJ model. At the end of this period, maxillary left molar teeth were extracted. In the first group, the subjects received no treatment. In the negative control group, poloxamer hydrogel containing empty microspheres were immediately applied to the soft tissues surrounding the extraction socket. The treatment group-1 was treated with local injections of poloxamer hydrogel containing hPTH. The treatment group-2 was treated with a single local injection of poloxamer hydrogel containing hPTH-loaded chitosan microspheres. Both treatment groups received a total of 7 µg of hPTH at the end of the treatment protocol. Our study demonstrates successful attenuation of MRONJ through a local drug delivery system combined with hPTH, as opposed to previously attempted treatment strategies.