ABSTRACT
The article presents the results of biochemical evaluation of metabolic effects of lipophilic products of plant origin among which such oils as linseed, black nuts and walnuts oils as well as medicine «Phosphogliv¼ were selected as the most promising ones. The influence of the studied substances on lipid metabolism in experiment on male rats (170220 g body weight) with modeled acute hepatotoxicity with carbon tetrachloride (that was achieved by subcutaneous injection of 50% oil solution of carbon tetrachloride 0.5 ml/100 g of the body mass once a day during 3 days) has been investigated. Liver function was assessed by triacylglycerols content in the serum, total, esterified and nonesterified cholesterol, cholesterol in the lipoproteins of high, low and very low density, as well as by the nonesterified cholesterol and phospholipids content in the hemolysate of red blood cells. Carbon tetrachloride hepatotoxic damage was accompanied by the development of severe hypercholesterolemia associated both with the increase in total cholesterol and its content in low density lipoproteins alongside the reducing of the cholesterol concentration in high density lipoproteins, resulted in secondary dyslipoproteinemia. Inhibition of the esterification of cholesterol processes as well as the decrease in the triacylglycerols concentration was observed. It is connected with the triacylglycerols endogenous synthesis blocking in the liver, resulted from its toxic damage. It is also confirmed by cholesterol content reducing in the lipoproteins of very low density. In erythrocytes of rats with CCl4 intoxication phospholipid content decreased while the amount of nonesterified cholesterol that is a component of cell membranes, influencing the proteins and lipids diffusion, which reduces the mobility of the fatty acid residues of phospholipids, increased. The injection of the black nuts and walnuts oils as well as flax oil (intragastric injections 0.2 ml daily in the morning before the main feeding from the 7th to the 30th day of the experiment, n=25 in each group) to rats with liver failure induced by carbon tetrachloride, contributed to the partial restoration of liver tissue structure and statistically reliable decrease of lipid metabolism. Decrease in the total cholesterol content by 17.5% in the group of animals treated with linseed oil was observed; LDL cholesterol also decreased under the influence of walnuts and black nuts oils by 36.7 and 40.6% respectively. The increase in the content of phospholipids in erythrocytes of rats when administered by the studied lipophilic products has made it possible to prove the improvement of the cell membranes rheological properties. The results of the study of the influence of linseed, black nuts and walnuts oils as well as medicine «Phosphogliv¼ on animals with hepatotoxicity by CCl4 have proved positive effect of these lipophilic substances on lipid metabolism.
Subject(s)
Animal Feed , Glycyrrhizic Acid/pharmacology , Linseed Oil/pharmacology , Lipid Metabolism/drug effects , Liver/metabolism , Phosphatidylcholines/pharmacology , Animals , Carbon Tetrachloride Poisoning/metabolism , Carbon Tetrachloride Poisoning/pathology , Drug Combinations , Liver/pathology , Male , RatsABSTRACT
In the article the influence of the hepatotropic effect of linseed and walnut oils on the lipid composition of the rats' blood serum under CCl4 intoxication has been presented. Lipid metabolism was characterised by the assessment of total cholesterol content in the blood serum, non-etherified cholesterol, cholesterol ethers, cholesterol lipoproteids of high-, low- and very low- density as well as triaclglycerins. In rats with experimental severe liver insufficiency associated with cholesterol increase in low-density lipoproteid fraction alongside its decrease in high-density lipoproteid fraction hypercholesterolemia was progressing. Cholesterol etherification inhibition (by 31.1%) was also observed. The tendency to less intensive disorders (by 1.7-2.0) as well as the tendency to lipid metabolism normalisation in animals have been revealed under the influence of lipophilic substances studied.
Subject(s)
Carbon Tetrachloride Poisoning/blood , Carbon Tetrachloride Poisoning/drug therapy , Cholesterol/blood , Juglans , Linseed Oil/pharmacology , Lipid Metabolism/drug effects , Animals , Male , RatsSubject(s)
Antioxidants/chemistry , Liposomes/chemistry , Plant Oils/chemistry , Corn Oil/chemistry , Diterpenes , Juglans/chemistry , Linseed Oil/chemistry , Models, Biological , Olive Oil , Phosphatidylcholines/chemistry , Retinyl Esters , Sunflower Oil , Thiobarbiturates/chemistry , Vitamin A/analogs & derivatives , Vitamin A/chemistryABSTRACT
The results of the study of activity of digestive proteases (pepsin, trypsin, chymotrypsin) in homogenates of stomach, pancreas and duodenum in experimental animals have been presented. Rats were exposed to intoxication with carbon tetrachloride (subcutaneous administration of a 50% oil solution of CCl4 in the dose of 0.5 ml per 100 g body weight) for three days and then they were given analysed oils (black nut, walnut and flax oil) intragastrically by gavage at a dose of 0.2 ml per day within 23 days. Pepsin level in gastric mucosa homogenates and chymotrypsin activity in pancreatic homogenates were determined by method of N.P. Pyatnitskiy based on on the ability of enzymes to coagulate dairy-acetate mixture, respectively, at 25 degrees C and 35 degrees C. Trypsin activity in homogenates of pancreatic was determined by method of Erlanger - Shaternikova colorimetrically. It has been established that intoxication with CCl4 decreased the synthesis of proteolytic enzymes of the stomach (by 51%) and pancreas (by 70-78%). Injections of analysed vegetable oils to animals contributed to the normalization of proteolytic enzymes synthesis. The conclusion that there are prospects of using the analysed vegetable oils containing large quantity of polyunsaturated fatty acids (omega-3 and omega-6) for the correction of detected biochemical abnormalities has been done.
