ABSTRACT
The authors performed a comparative immunohistochemical study of placental vascular endothelial growth factor (VEGF) and its receptors (VEGFR-1, VEGFR-2 and VEGFR-3) in gestational diabetes mellitus and type 1 diabetes. The expression of VEGF and its receptors was ascertained to be increased in the placental terminal and stem villi, mostly in type 1 diabetes. The changes found in the immunophenotype of terminal and stem villi suggest that the placenta is damaged and this may accordingly lead to intrauterine growth disturbances in the fetus and further to diseases in the neonatal infant.
Subject(s)
Diabetes Mellitus, Type 1/metabolism , Diabetes, Gestational/pathology , Vascular Endothelial Growth Factor A/metabolism , Vascular Endothelial Growth Factor Receptor-1/metabolism , Vascular Endothelial Growth Factor Receptor-2/metabolism , Vascular Endothelial Growth Factor Receptor-3/metabolism , Adult , Chorionic Villi/metabolism , Diabetes Mellitus, Type 1/complications , Diabetes Mellitus, Type 1/pathology , Diabetes, Gestational/metabolism , Endothelial Growth Factors/metabolism , Female , Gene Expression Regulation , Humans , Immunohistochemistry , Infant , Placenta/metabolism , Placenta/pathology , Pregnancy , Receptor Protein-Tyrosine Kinases/metabolism , Trophoblasts/metabolism , Trophoblasts/pathologyABSTRACT
We performed a morphological study of placentas from women with type 1 diabetes mellitus receiving insulin therapy (insulin pump). Expression of vascular endothelial growth factor (VEGF) and its receptors (VEGFR-1, VEGFR-2, VEGFR-3) was demonstrated by immunohistochemical methods. Processes of branched angiogenesis predominated in the placentas from women with type 1 diabetes mellitus. Immunohistochemical study revealed more intensive reaction of VEGF and its receptors in syncytiotrophoblast and capillary endothelium of terminal villi.
