Glial Cell Line-Derived Neurotrophic Factor-Loaded CMCht/PAMAM Dendrimer Nanoparticles for Peripheral Nerve Repair.

(1) Background: Peripheral nerve injuries represent a major clinical challenge. If nerve ends retract, there is no spontaneous regeneration and grafts are required to proximate the nerve ends and give continuity to the nerve. (2) Methods: GDNF-loaded NPs were characterized physicochemically. For that, NPs stability at different pH's was assessed, and GDNF release was studied through ELISA. In vitro studies are performed with Schwann cells, and the NPs are labeled with fluorescein-5(6)-isothiocyanate for uptake experiments with SH-SY5Y neural cells. (3) Results: GDNF-loaded NPs are stable in physiological conditions, releasing GDNF in a two-step profile, which is beneficial for nerve repair. Cell viability is improved after 1 day of culture, and the uptake is near 99.97% after 3 days of incubation. (4) Conclusions: The present work shows the efficiency of using CMCht/PAMAM NPs as a GDNF-release system to act on peripheral nerve regeneration.

Nanoparticles for neurotrophic factor delivery in nerve guidance conduits for peripheral nerve repair.

Peripheral nerve injuries are a major source of disabilities, and treatment of long nerve gap autografts is the gold standard. However, due to poor availability and donor-site morbidity, research is directed towards the development of regenerative strategies based on the use of artificial nerve guidance conduits (NGCs). Several properties and characteristics of the NGCs can be fine-tuned, such as the architecture of the conduit, the surface topography and the addition of bioactive molecules and cells to speed up nerve regeneration. In this review, US FDA-approved NGCs are described. The recent works, in which polymeric, magnetic, silica-based and lipidic NPs are employed to introduce growth factors (GFs) to NGCs, are overviewed and discussed in depth herein.

Nerves present in the extremities of the body are often injured, and this can lead to disabilities. To treat this problem, nerve sections from other body parts can be used, but the main disadvantage of this technique is poor availability and donor-site morbidity. To tackle these difficulties, research is focused on the development of artificial nerves, which are known as nerve guidance conduits (NGCs). This review article focuses on advances in this field, which is mainly related to the optimization of the material for conduit synthesis, on architecture and topography, and on how the functionalization of the NGCs with bioactive molecules can support nerve regeneration at the injured site. Currently commercialized NGCs are presented, and an in-depth discussion on strategies comprising neurotrophic factors administered alone, or included in the NGCs using nanoparticles, is also provided.

Antibacterial Layer-by-Layer Coatings for Medical Implants.

The widespread occurrence of nosocomial infections and the emergence of new bacterial strands calls for the development of antibacterial coatings with localized antibacterial action that are capable of facing the challenges posed by increasing bacterial resistance to antibiotics. The Layer-by-Layer (LbL) technique, based on the alternating assembly of oppositely charged polyelectrolytes, can be applied for the non-covalent modification of multiple substrates, including medical implants. Polyelectrolyte multilayers fabricated by the LbL technique have been extensively researched for the development of antibacterial coatings as they can be loaded with antibiotics, antibacterial peptides, nanoparticles with bactericide action, in addition to being capable of restricting adhesion of bacteria to surfaces. In this review, the different approaches that apply LbL for antibacterial coatings, emphasizing those that can be applied for implant modification are presented.

Highly Selective and Sharp Volcano-type Synergistic Ni2Pt@ZIF-8-Catalyzed Hydrogen Evolution from Ammonia Borane Hydrolysis.

Ammonia borane hydrolysis is considered as a potential means of safe and fast method of H2 production if it is efficiently catalyzed. Here a series of nearly monodispersed alloyed bimetallic nanoparticle catalysts are introduced, optimized among transition metals, and found to be extremely efficient and highly selective with sharp positive synergy between 2/3 Ni and 1/3 Pt embedded inside a zeolitic imidazolate framework (ZIF-8) support. These catalysts are much more efficient for H2 release than either Ni or Pt analogues alone on this support, and for instance the best catalyst Ni2Pt@ZiF-8 achieves a TOF of 600 molH2·molcatal-1·min-1 and 2222 molH2·molPt-1·min-1 under ambient conditions, which overtakes performances of previous Pt-base catalysts. The presence of NaOH boosts H2 evolution that becomes 87 times faster than in its absence with Ni2Pt@ZiF-8, whereas NaOH decreases H2 evolution on the related Pt@ZiF-8 catalyst. The ZIF-8 support appears outstanding and much more efficient than other supports including graphene oxide, active carbon and SBA-15 with these nanoparticles. Mechanistic studies especially involving kinetic isotope effects using D2O show that cleavage by oxidative addition of an O-H bond of water onto the catalyst surface is the rate-determining step of this reaction. The remarkable catalyst activity of Ni2Pt@ZiF-8 has been exploited for successful tandem catalytic hydrogenation reactions using ammonia borane as H2 source. In conclusion the selective and remarkable synergy disclosed here together with the mechanistic results should allow significant progress in catalyst design toward convenient H2 generation from hydrogen-rich substrates in the close future.

Electron- and Hydride-Reservoir Organometallics as Precursors of Catalytically Efficient Transition Metal Nanoparticles in Water.

Nanoparticles (NPs) are actively investigated for their efficient use in catalysis, but their means of synthesis is a key factor influencing their catalytic properties owing to surface coverage with byproducts. Here, neutral electron- and hydride-rich late transition metal organometallics are compared for the synthesis of late transition metal NPs in the presence of poly(vinylpirolidone) (PVP). In particular, the effect of electron-reservoir donors, hydride-reservoir donors, and electron-rich dimers yielding NPs electrostatically stabilized by cationic organometallics are compared in terms of NP size and catalytic efficiency. The catalytic reactions scrutinized with excellent results include 4-nitrophenol reduction to 4-aminophenol by NaBH4 for the AuNPs and PdNPs, and Suzuki-Miyaura reactions for the PdNPs. The nature of the reductant has more influence on the NP size in the case of AuNPs than PdNPs, and the best NP catalysts are obtained with hydride-reservoir complexes as reductants. The less bulky hydride donors are superior, with the complex [CoCp(ŋ4 -C5 H6 )] (Cp=ŋ5 -C5 H5 ) giving the NPs with the best catalyst efficiencies for both reactions. Protection of the NP cores by the organometallic sandwich salt is found to be the key to catalytic efficiency.

Exploring the pH Sensitivity of Poly(allylamine) Phosphate Supramolecular Nanocarriers for Intracellular siRNA Delivery.

Silencing RNA (siRNA) technologies emerge as a promising therapeutic tool for the treatment of multiple diseases. An ideal nanocarrier (NC) for siRNAs should be stable at physiological pH and release siRNAs in acidic endosomal pH, fulfilling siRNA delivery only inside cells. Here, we show a novel application of polyamine phosphate NCs (PANs) based on their capacity to load negatively charged nucleic acids and their pH stability. PANs are fabricated by complexation of phosphate anions from phosphate buffer solution (PB) with the amine groups of poly(allylamine) hydrochloride as carriers for siRNAs. PANs are stable in a narrow pH interval, from 7 to 9, and disassemble at pH's higher than 9 and lower than 6. siRNAs are encapsulated by complexation with poly(allylamine) hydrochloride before or after PAN formation. PANs with encapsulated siRNAs are stable in cell media. Once internalized in cells following endocytic pathways, PANs disassemble at the low endosomal pH and release the siRNAs into the cytoplasm. Confocal laser scanning microscopy (CLSM) images of Rhodamine Green labeled PANs (RG-PANs) with encapsulated Cy3-labeled siRNA in A549 cells show that siRNAs are released from the PANs. Colocalization experiments with labeled endosomes and either labeled siRNAs prove the translocation of siRNAs into the cytosol. As a proof of concept, it is shown that PANs with encapsulated green fluorescence protein (GFP) siRNAs silence GFP in A549 cells expressing this protein. Silencing efficacy was evaluated by flow cytometry, CLSM, and Western blot assays. These results open the way for the use of poly(allylamine) phosphate nanocarriers for the intracellular delivery of genetic materials.

Exposure to air boosts CuAAC reactions catalyzed by PEG-stabilized Cu nanoparticles.

New PEG-stabilized CuNP catalysts are designed upon Cu(ii) reduction with sodium naphthalenide in MeCN followed by simple purification using the salting-out effect. Their catalytic activity in CuAAC is boosted upon 30 min exposure to air, producing Cu2O NPs. These NPs are also supported on SBA-15, providing excellent recyclable heterogeneous catalysts that are applied in low amounts for efficient "click" functionalization.

Electrostatic Assembly of Functional and Macromolecular Ferricinium Chloride-Stabilized Gold Nanoparticles.

Substituted ferrocenes with various stereoelectronic effects including a ferrocene-terminated dendrimer in ether reduce aqueous HAuCl4 to gold nanoparticles (AuNPs) by interfacial electron transfer. The dependence on the stirring speed plays a crucial role, and the stereoelectronic influences on the reaction rates are dramatic. With a ferrocene-containing polymer, the reaction is conducted using an homogeneous THF/water medium, also forming AuNPs. Fully stable functional, dendritic and polymeric ferricinium chloride-stabilized AuNPs are obtained with core sizes between 13 and 35 nm, an optimal size range for potential biomedical applications. Finally the ferricinium coating of the Au nanoparticles is replaced by a more electron-rich ferricinium derivative by exergonic redox reaction with the corresponding ferrocene derivative.

One-Step Synthesis of Mesoporous Silica Thin Films Containing Available COOH Groups.

Inorganic-organic hybrid mesoporous silica thin films with covalently bonded carboxylic acid groups were synthesized in a one-step procedure, using carboxylic-derivatized alkoxysilanes obtained by photochemical radical thiol-ene addition (PRTEA). The organosilanes were synthesized by clicking mercaptosuccinic or mercaptoacetic thioacids with vinyltrimethoxysilane, using benzophenone as the photoradical initiator. The films were synthesized by evaporation-induced self-assembly of a sol containing a mixture of tetraethoxysilane and different quantities of the organosilanes, without any further treatment after the PRTEA reaction. Two nonionic surfactants were used as templates to produce different pore sizes. Different aging times were also applied. Structural characterization with electron microscopy, porosimetry measurements, and small angle X-ray scattering with two-dimensional detection demonstrated the obtention of mesoporous phases whose degree of ordering depended on the amount of added organosilane. The incorporation of the functional silanes was shown by X-ray photoelectron spectroscopy, and the presence of the COOH groups was confirmed by Fourier transform infrared (FTIR). Finally, the availability of the COOH groups for further chemical modification was demonstrated by FTIR by following the changes in the typical carbonyl IR bands during proton exchange and metal complexation. The proposed simple methodology allows obtaining COOH-modified silica thin films in one step, without the need of hard reaction conditions or deprotection steps. Functionalization with carboxyl groups brings a pH-dependent switch-ability to the pore surface that can be used for multifunctional mesoporous materials design.