ABSTRACT
Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.
Subject(s)
Birnaviridae Infections/veterinary , Chickens , Infectious bursal disease virus/immunology , Lymphocytes/virology , Poultry Diseases/prevention & control , Sheep, Domestic , Viral Vaccines/immunology , Animals , Birnaviridae Infections/prevention & control , Birnaviridae Infections/virology , Cell Line , Poultry Diseases/virologyABSTRACT
Percutaneous access to a pelvic kidney was obtained by retrograde nephrostomy in combination with continuous observation and displacement of bowel loops via a laparoscope. A staghorn calculus was removed by ultrasonic lithotripsy and mechanical extraction, and the patient was discharged from the hospital 5 days later.
