Supercontinuum generation in a graded-index multimode tellurite fiber.

We report the generation of a broadband supercontinuum (SC) from 790 to 2900 nm in a tellurite graded-index (GRIN) multimode fiber with a nanostructured core. We study the SC dynamics in different dispersion regimes and observe near-single-mode spatial intensity distribution at high input energy values. Numerical simulations of the (3 + 1)D generalized nonlinear Schrödinger equation are in good agreement with our experiments. Our results open a new avenue for the generation of high-power mid-infrared SC sources in soft-glass fibers.

Atorvastatin on Treatment of Nonalcoholic Fatty Liver Disease Patients.

Non-alcoholic fatty liver disease (NAFLD) is a condition in which excess fat builds up in the liver, often related to obesity and insulin resistance, which can lead to inflammation and scarring of the liver tissue. While efforts have been made to develop effective treatments for NAFLD, the need for pharmaceutical interventions remains unmet. Large clinical trials investigating the association between statin use and NAFLD are scarce, leading to contradictory results. Statins play a crucial role in cholesterol synthesis in the liver. Several studies have demonstrated that statins possess anti-inflammatory, anti-thrombotic, and anti-fibrotic properties. These properties make statins potentially useful in preventing the progression of NAFLD from simple steatosis to more severe forms like non-alcoholic steatohepatitis (NASH) and fibrosis. The results indicate that statin use is associated with a lower prevalence of NASH and fibrosis and may have a preventive effect on NAFLD.

A Review of the Effect of Plasticizers on the Physical and Mechanical Properties of Alginate-Based Films.

In recent years, there has been a growing attempt to manipulate various properties of biodegradable materials to use them as alternatives to their synthetic plastic counterparts. Alginate is a polysaccharide extracted from seaweed or soil bacteria that is considered one of the most promising materials for numerous applications. However, alginate potential for various applications is relatively limited due to brittleness, poor mechanical properties, scaling-up difficulties, and high water vapor permeability (WVP). Choosing an appropriate plasticizer can alleviate the situation by providing higher flexibility, workability, processability, and in some cases, higher hydrophobicity. This review paper discusses the main results and developments regarding the effects of various plasticizers on the properties of alginate-based films during the last decades. The plasticizers used for plasticizing alginate were classified into different categories, and their behavior under different concentrations and conditions was studied. Moreover, the drawback effects of plasticizers on the mechanical properties and WVP of the films are discussed. Finally, the role of plasticizers in the improved processing of alginate and the lack of knowledge on some aspects of plasticized alginate films is clarified, and accordingly, some recommendations for more classical studies of the plasticized alginate films in the future are offered.

A systematic review of informatics competencies: requirements for nurse managers in healthcare organisations.

Introduction: One vital strategy for the success of healthcare organisations currently resides in the capacity of the nurse managers (NMs) to develop informatics competencies in management. Aim: The aim of this review was to synthesise the evidence related to the informatics competencies of NMs in healthcare settings. Methods: The databases of PubMed, Web of Science, Scopus and Education Resources Information Center were searched. All publications were accepted if they had been published in English and full texts were accessible. The study was carried out using a systematic literature search of work published between January 2000 and December 2021. The Endnote v.17 software was used to facilitate reference management. Quality assessment of publications was carried out independently by two reviewers using the Critical Appraisal Skills Programme guidance. A descriptive summary with data tables was produced to summarise the literature. Results: After screening the search results, 12 studies published between the period 2001 and 2019 met the inclusion criteria. Analysis of the included studies led to the classification of informatics competencies in three main themes: skills related to work with computers (four sub-themes), informatics skills of NMs (three sub-themes) and knowledge of NMs about informatics (five sub-themes). Conclusion: These systematic review results can lead to the identification of informatics competency gaps and recommend and provide solutions to address these gaps and increase competencies for nursing managers.

Atorvastatin and Flaxseed Effects on Biochemical Indices and Hepatic Fat of NAFLD Model in Rats.

Background: Non-alcoholic fatty liver disease (NAFLD) is one of the most common forms of chronic liver disease that affects about 25% of the general population. No definitive treatment for NAFLD has been identified yet. The aim was to determine the effect of atorvastatin (ATO) and flaxseed on related indicators of NAFLD-induced fat/fructose-enriched diet (FFD). Materials and Methods: Forty male Wistar rats were divided into five groups. NAFLD groups received FFD and carbon tetrachloride (CCl4) to induce NAFLD. After intervention with ATO (10 mg/kg/day) and/or flaxseed (7.5 g/kg/day), liver enzymes and lipid profiles in serum were determined at eight week of interventions. Results: Triglycerides (TG) and cholesterol (CHO) in FFD + ATO, FFD + flaxseed, and FFD + ATO + flaxseed had a significant decrease and low-density lipoprotein (LDL) level and LDL/high-density lipoprotein (HDL) ratio showed a significant increase in the FFD + flaxseed compared to the FFD. The levels of aspartate transaminase (AST), alanine transaminase (ALT), and gamma-glutamyltransferase (GGT) were significantly reduced in the FFD + ATO, FFD + flaxseed, and the FFD + ATO + flaxseed. In addition, Alkaline phosphatase (ALP) levels were significantly different between normal and FFD. Fasting blood sugar (FBS) levels were significantly different in the FFD + flaxseed and the FFD + ATO + flaxseed compared to the FFD. Conclusion: ATO therapy along with flaxseed controls NAFLD-related indices and FBS. Therefore, it can be stated with caution that ATO and flaxseed can be used to improve lipid profile and reduce the complications of NAFLD.

Effect of date seed (Phoenix dactylifera) supplementation as functional food on cardiometabolic risk factors, metabolic endotoxaemia and mental health in patients with type 2 diabetes mellitus: a blinded randomised controlled trial protocol.

INTRODUCTION: Recently, the improvement of chronic hyperglycaemia-related damage of type 2 diabetes mellitus (T2DM) through functional food consumption has attracted the attention of many clinicians. This study aims to determine the effectiveness of date seed powder (DSP) as a functional food (prebiotic) on the cardiometabolic risk factors, oxidative stress, anti-/inflammatory biomarkers, metabolic endotoxaemia (gut microbiota), adipokines, hypothalamic-pituitary-adrenal axis biomarkers, immune system, anthropometric indices and mental health in patients with T2DM. METHODS: This study protocol will be conducted as randomised, triple-blind, placebo-controlled trial with the inclusion of 48 patients with T2DM. The participants will be randomly assigned into two equal groups of intervention (n=24) and placebo (n=24) and receive 5 g/day of DSP or placebo for 8 weeks, respectively. At baseline and post-intervention, fasting blood samples will be collected to assess the serum levels of lipid profile, glycaemic indices, antioxidant and oxidative stress, anti-/inflammatory biomarkers, lipopolysaccharide, 8-hydroxy-guanine, adipokines, hypothalamic-pituitary-adrenal axis biomarkers, immune system and mental health. Data will be analysed using the SPSS software (V.16.0). To compare the quantitative variables, paired and unpaired Student's t-tests and covariance analyses will be used. DISCUSSION: In this study, the potential effects of DSP on patients with T2DM will be evaluated for the first time. It is hoped that the results would increase the body of scientific knowledge about DSP supplementation on the cardiometabolic risk factors, oxidative stress, anti-/inflammatory biomarkers, metabolic endotoxaemia, adipokines, hypothalamic-pituitary-adrenal axis biomarkers, immune system, anthropometric indices and mental health in patients with T2DM. ETHICS AND DISSEMINATION: The study protocol was approved by the Ethical Committee of the Tabriz University of Medical Sciences, Tabriz, Iran (code: IR.TBZMED.REC.1400.752). TRIAL REGISTRATION NUMBER: Iranian Registry of Clinical Trials (www.irct.ir/IRCT20150205020965N10).

Developing inpatient suicide prevention strategies in medical settings: Integrating literature review with expert testimony.

OBJECTIVE: According to some recent evidence, suicide rate is higher in inpatients than in the general population around the world. However, suicide prevention strategies (SPS) are poorly focused and understood in medical settings. This study aimed to develop effective SPS and interventions in medical settings of Iran and provide evaluation checklists/procedures for them. METHODS: The study was performed in two steps, including literature review and expert opinions panel. In the first stage, we conducted a comprehensive literature review to find relevant suicide prevention programs, strategies, interventions, or any efforts to prevent suicide in the medical settings. In the second stage, an expert panel was arranged for developing effective and feasible SPS in medical settings. Data were analyzed through content analysis approach. RESULTS: Overall, 11 records were included in the literature review. SPS varied from staff training, safety plan and quality improvements, and prevention programs to therapy methods. Finally, in the second stage, the following seven major SPS were recommended by the expert panel: 1) Integration and application of obtained suicidal behavior data through evaluating medical records, 2) Screening and suicide risk assessment, 3) Staff training, 4) Quality improvement, 5) Follow-up of inpatients with high-risk behaviors, 6) Reducing stigma and improving public awareness, and 7) Follow-up of discharged high-risk inpatients. Also, 23 interventions within the strategies were presented. CONCLUSION: Given that SPS are poorly focused in medical settings, the practical framework that emerged in this study could be used to develop or advance SPS in various medical settings.

Two octave supercontinuum generation in a non-silica graded-index multimode fiber.

The generation of a two-octave supercontinuum from the visible to mid-infrared (700-2800 nm) in a non-silica graded-index multimode fiber is reported. The fiber design is based on a nanostructured core comprised of two types of drawn lead-bismuth-gallate glass rods with different refractive indices. This yields an effective parabolic index profile and ten times increased nonlinearity when compared to silica fibers. Using femtosecond pulse pumping at wavelengths in both normal and anomalous dispersion regimes, a detailed study is carried out into the supercontinuum generating mechanisms and instabilities seeded by periodic self-imaging. Significantly, suitable injection conditions in the high power regime are found to result in the output beam profile showing clear signatures of beam self-cleaning from nonlinear mode mixing. Experimental observations are interpreted using spatio-temporal 3+1D numerical simulations of the generalized nonlinear Schrödinger equation, and simulated spectra are in excellent agreement with experiment over the full two-octave spectral bandwidth. Experimental comparison with the generation of supercontinuum in a silica graded-index multimode fiber shows that the enhanced nonlinear refractive index of the lead-bismuth-gallate fiber yields a spectrum with a significantly larger bandwidth. These results demonstrate a new pathway towards the generation of bright, ultrabroadband light sources in the mid-infrared.

Supercontinuum intensity noise coupling in Fourier transform photoacoustic spectroscopy.

We investigate the noise transfer mechanism from the light source intensity fluctuations to the acoustic signal in Fourier transform photoacoustic spectroscopy (FT-PAS). This noise coupling is expected to be reduced in FT-PAS compared with conventional Fourier transform spectroscopy, as only the specific spectral components that are absorbed by the probed sample contribute to the noise level. We employ an incoherent supercontinuum (SC) light source in our experiments and observe a linear relation between the sample gas concentration and the detected noise level, which significantly reduces the influence of the SC noise on the detection limit. Based on our experimental results, we derive a model for the noise level, which establishes the foundation for practical sensitive implementation of FT-PAS.

Evaluation of pesticide residues and risk assessment in apple and grape from western Azerbaijan Province of Iran.

West Azerbaijan, especially the city of Urmia, is the center of Iranian apple and grape production hence the importance of this, residues of 85 pesticides in these products were investigated using modified QuEChERS extraction followed by UHPLC-MS/MS technique. Residues of 17 different pesticides detected in some apple samples. In grape sample only 7 pesticides detected. The levels of the residues found in all apple and grape samples were below the maximum residue levels (MRLs) of Iran, except for iprodione. Health risk assessment associated with pesticide residues in apples and grapes were estimated by hazard quotient (HQ) and hazard index (HI), which indicated that the HI value was lower than 1 in adults and children due to apple and consumption. HI in adults and children were 0.012 and 0.054 in apple; 0.001 and 0.003 in grape samples, respectively. Although the health risk assessment showed that the consumers are not at considerable risk but due to pesticide residue, implement control plans to manage the proper application of this pesticide, or replace it with safer alternatives in apple and grape is required.

Pesticide residues in green-house cucumber, cantaloupe, and melon samples from Iran: A risk assessment by Monte Carlo Simulation.

This research aims to analyze 56 pesticide residues in 100 green-house cucumber and 150 cantaloupe and melon samples collected from markets in Iran by Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) extraction method based on analysis with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). In addition, non-carcinogenic and carcinogenic probabilistic health risk assessments were evaluated by Hazard Quotient (HQ), Hazard Index (HI), and Carcinogenic Risk (CR) based on the Monte Carlo Simulation (MCS) method. According to Iranian regulation, 18% of cucumber and 22% of cantaloupe and melon samples were contaminated by at least one pesticide. Arrange pesticides based on HQ ranking was diazinon > thiacloprid > imidacloprid tebuconazole for cucumbers and chlorpyrifos > thiacloprid > imidacloprid > metalaxyl for cantaloupes and melons. In addition, HI for adults was 0.067 in cucumber and 0.24 in cantaloupe and melon; and for children, 0.30 in cucumber and 0.60 in cantaloupe and melon. Arrange pesticides based on CR ranking was thiacloprid > imidacloprid > metalaxyl > chlorpyrifos > tebuconazole > diazinon. However, CR was more than 1.0E-6 value due to thiacloprid, so consumers were at considerable carcinogenic risk in these commodities.

Evaluation of nitrogen and heavy metal pollution in southern Caspian Sea: Risk assessment and modeling approach.

Industrial, agricultural, and recreational activities dump several pollutants into the Caspian Sea, which is one of the main water bodies of Iran. Therefore, performing risk assessments would be required as part of the monitoring programs. Herein, non-cancer health risk assessment of the consumption of macroalgae was performed and the ecological risk assessment of metal pollution in sediments of the southern Caspian Sea was presented using sediment quality guidelines (SQGs), enrichment factors (EF), contamination factors (CF), contamination degrees (CD), pollution load indices (PLI), geo accumulation indices (Igeo), and potential ecological risk indices (RI). Next, machine-learning approaches were used to predict the stable isotope value of nitrogen in macroalgae, in which physicochemical information of water and heavy metal levels in macroalgae were used separately and together as predictor variables. Results indicated that simultaneous use of physicochemical properties of water and heavy metal levels resulted in the best prediction of isotopic nitrogen content.

Probabilistic health risk assessment based on Monte Carlo simulation for pesticide residues in date fruits of Iran.

The validation of an analytical procedure based on the quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach is presented for multiresidue analysis of pesticides in dates by liquid chromatography-tandem mass spectrometry (UHPLC-MS2). The proposed methodology was applied for simultaneous quantification of 16 pesticides in 50 different date fruits. Method validation was performed regarding accuracy, precision, LDR, LOD, and LOQ, as well as matrix effects. Results of validation were satisfactory, with recoveries higher than 80% for 75% of the samples for 100- and 500- µg L-1 spike levels. Evaluation of the matrix effect revealed that for 81% of the samples, a slight matrix effect was observed. Residues in 92% of the real samples were found below national MRLs. Afterward, hazard quotient (HQ) and total hazard quotient (THQ) of human health risk assessment of pesticides was estimated using a probabilistic approach based on the Monte Carlo (MC) algorithm. Total hazard quotient (THQ) in adults based on the consumption of dates in total samples was estimated to be 7.8% and 36.7% for adults and children, respectively. Since the studied pesticides are registered in the country and are the most widely used pesticides on dates, the occurrence of other pesticide residues seems to be unlikely. Consequently, the applied health risk assessment on Iranian date fruit samples showed that the HQ for adults and children populations indicates no risk to human health.

Production of recombinant lethal factor of Bacillus anthracis in Bacillus subtilis.

Cancer is considered as a disease with high rates of mortality and morbidity. The limitations and side effects of common treatments have prompted the need for innovative cancer therapies. Furthermore, selectivity and targeting of cancer cells are crucial factors to successful treatment of cancer. One of these methods is the use of bacterial toxins including Bacillus anthracis toxin to aid cancer therapy. This toxin is composed of three polypeptides: protective factor (PA), lethal factor (LF), and edema factor (EF). PA can bind to various surface receptors of all types of human cells and it internalizes the lethal factor and edema factor subunits of the toxin in the cytosol. In the present study, we cloned and expressed the lef gene of B. anthracis as the lethal part of the toxin in Bacillus subtilis WB600 by a shuttle expression vector PHT4. The rLF made in B. subtilis is efficiently secreted by the host into the culture medium which facilitates downstream processing. The rLF can be used to study cancer treatment. Abbreviations: EF: edema factor; LF: lethal factor; PA: protective factor; rLF: recombinant lethal factor; rPAm: recombinant protective factor mutants; uPA: urokinase-type plasminogen activator; uPAR: urokinase-type plasminogen activator receptor.

Low-noise octave-spanning mid-infrared supercontinuum generation in a multimode chalcogenide fiber.

We demonstrate the generation of a low-noise, octave-spanning mid-infrared supercontinuum from 1700 to 4800 nm by injecting femtosecond pulses into the normal dispersion regime of a multimode step-index chalcogenide fiber with 100 µm core diameter. We conduct a systematic study of the intensity noise across the supercontinuum spectrum and show that the initial fluctuations of the pump laser are at most amplified by a factor of three. We also perform a comparison with the noise characteristics of an octave-spanning supercontinuum generated in the anomalous dispersion regime of a multimode fluoride fiber with similar core size and show that the normal dispersion supercontinuum in the multimode chalcogenide fiber has superior noise characteristics. Our results open up novel perspectives for many practical applications such as long-distance remote sensing where high power and low noise are paramount.

Recent developments in liquid chromatography-mass spectrometry analyses of ghrelin and related peptides.

Profiling and monitoring concentrations of key hormones in body have long been critical aims in clinical therapy. As a crucial hormone, identification and quantification of ghrelin is a fundamental, often key, step in understanding human physiological mechanisms. Through the advances and improvements of different analytical techniques, ghrelin measurement is generally feasible, and the number of successful reports is progressively being increased with new aspects of selectivity, sensitivity and ease of use in various circumstances. Herein we discuss current chromatographic methods for sample collection, separation and a mass spectrometry method for detection and measurement of ghrelin and other proghrelin-derived peptides in biological metrics. We describe the most commonly applied analytical LC-MS procedures for determination of proghrelin-derived peptides and provide illustrative instances representing the state of the art. This review is intended for bioanalytical chemists or clinical researchers who are interested in this field of research.

Simple and Sensitive Quantification of Ghrelin Hormone in Human Plasma Using SBSE-HPLC/DAD-MS.

During a decade and more since its discovery, the emerging physiological roles of ghrelin in mammalian are increasingly being introduced, proposing a critical need for its quantification in biological milieu. Here in, a simple and sensitive single-step method for extraction and quantification of ghrelin in human plasma was developed and validated using stir bar sorptive extraction (SBSE) followed by high-performance liquid chromatography (HPLC) with diode array detection (DAD) coupled to mass spectrometry (MS). Influential parameters of SBSE procedure were optimized including extraction and desorption times of 45 and 30 min, respectively; pH of 4; no addition of salt. The sum of peak heights of three most intense selected ions in mass spectrum (844, 1125 and 1686 m/z) related to 4-, 3- and 2-fold-charged ions of ghrelin was used for quantification. Validation parameters containing linear dynamic range, limit of quantification and limit of detection were 0.02-80, 0.02 and 0.007 µg L-1, respectively, and calculated relative standard deviation for peak heights was 6.5% (0.7 µg L-1 standard solution). Mean recovery for ghrelin in spiked plasma samples was 96% ± 3. The efficiency of the SBSE-HPLC/DAD-MS procedure was proved by analysis of plasma samples from obese patients undergoing gastric plication surgery. The suggested methodology would contribute to simple and fast analysis of ghrelin levels in obesity and related diseases and also biochemical cycles in which gherlin is present.

Fabrication of channel waveguides in dye-doped polymer films by a beam-processing machine based on photo induced bleaching.

Irreversible changes in the refractive index of a dye-doped polymer film by photo induced bleaching were measured with a Mach-Zehnder interferometer. The decrease in the refractive index of the film for various laser powers and various radiation times has been investigated. A beam-processing machine with two simultaneous outputs has been designed to make use of this change in the refractive index to fabricate a channel waveguide. This method is simple and one step, and does not involve any solvent or mask; and also due to photobleaching, the pattern is not reversible.