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Plant Biotechnol J ; 10(3): 341-52, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22070155

ABSTRACT

Edible fruits are inexpensive biofactories for human health-promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) selected against the VP8* peptide of rotavirus SA11 strain were obtained. The amount of hIgA_2A1 protein reached 3.6 ± 0.8% of the total soluble protein in the fruit of the transformed plants. Minimally processed fruit-derived products suitable for oral intake showed anti-VP8* binding activity and strongly inhibited virus infection in an in vitro virus neutralization assay. In order to make tomatoes expressing hIgA_2A1 easily distinguishable from wild-type tomatoes, lines expressing hIgA_2A1 transgenes were sexually crossed with a transgenic tomato line expressing the genes encoding Antirrhinum majus Rosea1 and Delila transcription factors, which confer purple colour to the fruit. Consequently, transgenically labelled purple tomato fruits expressing hIgA_2A1 have been developed. The resulting purple-coloured extracts from these fruits contain high levels of recombinant anti-rotavirus neutralizing human IgA in combination with increased amounts of health-promoting anthocyanins.


Subject(s)
Antibodies, Neutralizing/immunology , Plants, Genetically Modified/immunology , RNA-Binding Proteins/immunology , Rotavirus/immunology , Solanum lycopersicum/immunology , Viral Nonstructural Proteins/immunology , Agrobacterium tumefaciens/genetics , Agrobacterium tumefaciens/metabolism , Anthocyanins/metabolism , Antibodies, Monoclonal/immunology , Antibodies, Viral/immunology , Antirrhinum/genetics , Blotting, Western , Crosses, Genetic , Genetic Vectors/genetics , Genetic Vectors/metabolism , Humans , Immunoglobulin A/immunology , Immunoglobulin Fragments/genetics , Immunoglobulin Fragments/immunology , Solanum lycopersicum/genetics , Solanum lycopersicum/virology , Neutralization Tests , Pigmentation , Plant Extracts/immunology , Plants, Genetically Modified/genetics , Plants, Genetically Modified/virology , Plasmids/genetics , Plasmids/metabolism , RNA-Binding Proteins/genetics , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Recombinant Proteins/metabolism , Staining and Labeling , Transcription Factors/genetics , Transcription Factors/metabolism , Transgenes , Viral Nonstructural Proteins/genetics
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