Fecal calcium levels of bird nestlings as a potential indicator of species-specific metal sensitivity.

Sensitivity of bird species to environmental metal pollution varies but there is currently no general framework to predict species-specific sensitivity. Such information would be valuable from a conservation point-of-view. Calcium (Ca) has antagonistic effects on metal toxicity and studies with some common model species show that low dietary and circulating calcium (Ca) levels indicate higher sensitivity to harmful effects of toxic metals. Here we measured fecal Ca and five other macroelement (potassium K, magnesium Mg, sodium Na, phosphorus P, sulphur S) concentrations as proxies for dietary levels in 66 bird species to better understand their interspecific variation and potential use as an indicator of metal sensitivity in a wider range of species (the main analyses include 39 species). We found marked interspecific differences in fecal Ca concentration, which correlated positively with Mg and negatively with Na, P and S levels. Lowest Ca concentrations were found in insectivorous species and especially aerial foragers, such as swifts (Apodidae) and swallows (Hirundinidae). Instead, ground foraging species like starlings (Sturnidae), sparrows (Passeridae), cranes (Gruidae) and larks (Alaudidae) showed relatively high fecal Ca levels. Independent of phylogeny, insectivorous diet and aerial foraging seem to indicate low Ca levels and potential sensitivity to toxic metals. Our results, together with information published on fecal Ca levels and toxic metal impacts, suggest that fecal Ca levels are a promising new tool to evaluate potential metal-sensitivity of birds, and we encourage gathering such information in other bird species. Information on the effects of metals on breeding parameters in a wider range of bird species would also help in ranking species by their sensitivity to metal pollution.

A First Record of Organochlorine Pesticides in Barn Owls (Tyto alba) from Portugal: Assessing Trends from Variation in Feather and Liver Concentrations.

We evaluated feathers as a non-destructive biomonitoring tool documenting organochlorine pesticides (OCP) in liver and checked possible trends in pesticide use in two areas based on OCP concentrations in barn owls (Tyto alba). We measured the concentrations of 16 OCP in 15 primary feathers and 15 livers from barn owl carcasses collected on roadsides in Tagus Valley and Évora regions, south Portugal. Total OCP mean concentration was 8 120 ng g-1 in feathers and 178 ng g-1 in livers. All compounds were detected in feathers while in livers δ-HCH, endosulfan sulphate, p,p'-DDT and p,p'-DDD were not detected. The high ß-HCH and heptachlor concentrations in feathers most likely derived from external endogenous contamination. P,p'-DDE was the OCP with the highest hepatic concentration. Both matrices indicated an exposure to recently released heptachlor. The differing OCP concentrations between Tagus Valley and Évora seem to reflect differences in land-use and pesticide use histories of the two locations, and/or faster degradation of OCP in the Tagus area.

A microplate adaptation of the thiobarbituric acid reactive substances assay to determine lipid peroxidation fluorometrically in small sample volumes / Una adaptación de microplacas del ensayo de sustancias reactivas del ácido tiobarbitúrico para determinar la peroxidación lipídica fluorométricamente en pequeños volúmenes de muestra

A simple, fast, reproducible and low-cost assay for thiobarbituric acid reactive substances (TBARS) has been adapted for use with a microplate spectrofluorometer. The technique allows rapid analysis of multiple samples and requires a very small sample volume (50 μl of red cell homogenates from passerine birds at protein concentrations of 3.4-8.9 mg/ml in this study), what is of special interest for biomonitoring studies working with small-sized animals from which a limited amount of sample can be obtained. The TBARS test involves the reaction of thiobarbituric acid (TBA) with malondialdehyde (MDA) under heating (90°C), leading to the formation of products that can be measured fluorometrically using black 384-well plates at excitation/emission wavelength of 532/553 nm. The concentrations of peroxidized lipids in samples were determined by extrapolation from a MDA standard curve. Two different excitation/emission combinations (532/553 and 530/550 nm) were used and both pairs were suitable for this technique. Intra- and inter-plate variability was < 20% and a good linearity of the standard curve was observed (R2 > 0.99). The research use of this microplate adaptation of the TBARS assay will provide further data and understanding of lipid peroxidation reducing the limitation of small sample volumen (AU)

El presente trabajo adapta un ensayo sencillo, rápido, reproducible y económico de sustancias reactivas al ácido tiobarbitúrico (TBARS) para su uso en espectrofluorómetro para microplacas. La técnica permite un análisis rápido de múltiples muestras y requiere un mínimo volumen de muestra (50 μl de un homogeneizado de eritrocitos de aves paseriformes a una concentración proteica de 3.4-8.9 mg/ml en este estudio), lo cual resulta de especial interés en estudios de biomonitorización que trabajan con animales de pequeño tamaño de los que se puede obtener una cantidad de muestra limitada. El ensayo TBARS consiste en la reacción del ácido tiobarbitúrico (TBA) con malondialdehído (MDA) en condiciones de calor (90°C), formando productos que pueden medirse fluorométricamente usando microplacas negras de 384 pocillos a 532/553 nm de excitación/emisión. La concentración de peróxidos lipídicos en la muestra se determinó por extrapolación de una curva de MDA. Se utilizaron dos combinaciones diferentes de excitación/emisión (532/553 and 530/550 nm) y ambas fueron apropiadas para la técnica. La variabilidad intra- e inter-placa fue <20% y se observó una buena linealidad de la curva estándar (R2 >0.99). El uso científico de la adaptación a microplaca del ensayo TBARS proporcionará más datos y comprensión sobre la peroxidación lipídica reduciendo la limitación que supone los pequeños volúmenes de muestra (AU)

Influence of a Former Mining Area in the Heavy Metals Concentrations in Blood of Free-Living Mediterranean Pond Turtles (Mauremys leprosa).

The objective of this study was to assess metal exposure in the Mediterranean Pond Turtle (Mauremys leprosa) inhabiting a watercourse in an ancient mining district polluted by different metals ("Rambla de Las Moreras", southeastern Spain) and included in the Ramsar Convention. For this purpose, mercury (Hg), lead (Pb), copper (Cu), zinc (Zn) and cadmium (Cd) were analysed in blood samples from 42 M. leprosa healthy adults (27 males and 13 females). The highest median concentrations were found for Zn, followed by Cu, Pb, Hg and Cd (366, 33, 9, 0.83 and 0.14 µg/dL, respectively). Although the literature regarding toxic metals in freshwater turtles is relatively scarce, Pb may cause deleterious effects in our population. In general, males presented higher levels than females, which could be due to maternal transfer during egg formation. The significant correlations between Cu-Cd and Cu-Hg suggest the implication of an efficient mechanism of detoxification involving metallothioneins.

Tracking pan-continental trends in environmental contamination using sentinel raptors-what types of samples should we use?

Biomonitoring using birds of prey as sentinel species has been mooted as a way to evaluate the success of European Union directives that are designed to protect people and the environment across Europe from industrial contaminants and pesticides. No such pan-European evaluation currently exists. Coordination of such large scale monitoring would require harmonisation across multiple countries of the types of samples collected and analysed-matrices vary in the ease with which they can be collected and the information they provide. We report the first ever pan-European assessment of which raptor samples are collected across Europe and review their suitability for biomonitoring. Currently, some 182 monitoring programmes across 33 European countries collect a variety of raptor samples, and we discuss the relative merits of each for monitoring current priority and emerging compounds. Of the matrices collected, blood and liver are used most extensively for quantifying trends in recent and longer-term contaminant exposure, respectively. These matrices are potentially the most effective for pan-European biomonitoring but are not so widely and frequently collected as others. We found that failed eggs and feathers are the most widely collected samples. Because of this ubiquity, they may provide the best opportunities for widescale biomonitoring, although neither is suitable for all compounds. We advocate piloting pan-European monitoring of selected priority compounds using these matrices and developing read-across approaches to accommodate any effects that trophic pathway and species differences in accumulation may have on our ability to track environmental trends in contaminants.

A review on exposure and effects of arsenic in passerine birds.

Arsenic (As) is a metalloid of high concern because of its toxic effects for plants and animals. However, it is hard to find information on this metalloid in passerines. This review presents a comprehensive overview of As exposure and effects in birds, and more particularly in passerines, as a result of an extensive search of the literature available. Internal tissues are the most frequently analyzed matrices for As determination in passerines (37.5% of the reviewed studies used internal tissues), followed by feathers and eggs (32.5% each), feces (27.5%), and finally blood (15%). A clear tendency is found in recent years to the use of non-destructive samples. Most studies on As concentrations in passerines have been done in great tit (Parus major; 50%), followed by pied flycatcher (Ficedula hypoleuca; 22.5%). Some factors such as diet and migratory status are crucial on the interspecific differences in As exposure. More studies are needed to elucidate if intraspecific factors like age or gender affect As concentrations in different tissues. The literature review shows that studies on As concentrations in passerines have been done mainly in the United States (30%), followed by Belgium (22.5%), and Finland (20%), making evident the scarce or even lack of information in some countries, so we recommend further research in order to overcome the data gap, particularly in the southern hemisphere. Studies on humans, laboratory animals and birds have found a wide range of effects on different organ systems when they are exposed to different forms of As. This review shows that few field studies on As exposure and effects in passerines have been done, and all of them are correlative so far. Arsenic manipulation experiments on passerines are recommended to explore the adverse effects of As in free-living populations at similar levels to those occurring in the environment. CAPSULE: This review summarizes the most interesting published studies on As exposure and effects in passerines.

Contaminants in the southern tip of South America: Analysis of organochlorine compounds in feathers of avian scavengers from Argentinean Patagonia.

The aim of this study was to assess the exposure to organochlorine compounds (OC) in 91 primary wing feathers of avian scavengers, Turkey vulture (Cathartes aura), American black vulture (Coragyps atratus) and Southern crested caracaras (Polyborus plancus) from the southern tip of South America, in the Argentinean Patagonia. We analyzed for a series of OC including hexachlorocyclohexane (HCH) isomers, endosulfan, aldrin, dieldrin, endrin, dichlorodiphenyltrichloroethane (p,p'-DDT), dichlorodiphenyldichloroethane (p,p'-DDD), dichlorodiphenyldichloroethylene (p,p'-DDE), heptachlor and heptachlor-epoxide. This is the first study on OC in feathers of three terrestrial top carnivores from South America. OC concentrations found in the studied species were much higher than those found in feathers of raptors from Europe and Asia, which likely indicate their high use in the region, specifically in agriculture, and other possible uses of OC in this area. ∑HCH had the highest median concentration, followed by ∑Drins, ∑DDT, ∑Heptachlor, and ∑Endosulfan, similar to those reported in several food samples in Argentina. On the other hand, differences in OC profiles between species and areas may be related to feeding and migratory habits, as well as the molt period. Three individuals showed ∑DDT (DDT, DDD and DDE) concentrations in feathers related to sublethal effects. However, this comparison should be used with caution due to problems with extrapolating such data across tissues and species.

Desarrollo de método analítico para la extracción de plaguicidas organoclorados en plumas / Development of an analytical method for extracting organochlorine pesticides from feathers

Debido a los efectos adversos de los plaguicidas organoclorados (OC), estos compuestos han sido monitorizados en diferentes especies de seres vivos. En estos estudios de biomonitorización ambiental, las aves han jugado un importante papel debido a su sensibilidad a los cambios ambientales y a su elevada posición en la cadena trófica. En los últimos años existe un interés creciente en utilizar muestras no destructivas como alternativa a los tejidos internos. En este sentido, las plumas han sido ampliamente utilizadas en la monitorización de la contaminación ambiental por metales pesados y podrían proporcionar información de la concentración de OC en la sangre durante su desarrollo. Sin embargo, la información sobre su uso en la monitorización de OC es escasa. El objetivo general del presente estudio es comprobar la utilidad de la pluma como unidad de biomonitorización de la exposición a plaguicidas organoclorados. Para ello se desarrolla un método de extracción de 16 OC en plumas, incluyendo α-, β- y δ-HCH, lindano, aldrín, dieldrín, endrín, endrín aldehído, endosulfán I y II, endosulfán sulfato, p,p’-DDT, DDD, DDE, heptacloro y su epóxido. Además se evalúa la interferencia por contaminación externa en los niveles encontrados en plumas y se estudia la distribución de los compuestos entre partes de la pluma (barbas y ejes).La contaminación externa parece tener influencia en los niveles encontrados en plumas para determinados compuestos, sin embargo, no parece ser la única causa de los mayores niveles observados en barbas en comparación con los ejes.La pluma parece ser una prometedora herramienta no destructiva de plaguicidas organoclorados en aves. Futuros estudios deben ir encaminados en determinar correlaciones entre concentraciones en plumas y tejidos internos de aves. Además, se deben evaluar factores adicionales como la edad, sexo y estado nutricional de las aves para comprobar su efecto sobre los niveles de contaminantes en plumas(AU)

Due to the adverse effects of organochlorine pesticides (OC), these compounds have been widely monitored in several species of living beings. Birds have played an important role in monitoring environmental pollution due to their sensitivity to environmental changes and their position in the upper of the food chain. In recent years, many efforts have been attempted to look for useful samples obtained in a non-destructive way as alternative to the collection of internal tissues. Feathers can provide information of OC concentrations in the circulating blood at the time of their development. They have been widely used in monitoring of metal environmental pollution. However, information about their use in OC monitoring is scarce. The general aim of this study is the validation of the feather as OC biomonitoring tool. In this sense, we develop a method of extraction for 16 OC in feathers, including α-, β-and δ-HCH, lindane, aldrin, dieldrin, endrin, endrin aldehyde, endosulfan I and II, endosulfan sulfate, p,p’-DDT, DDD, DDE, heptachlor and its epoxide. Moreover, we assess the influence of external contamination and the distribution of compounds between parts of the feather (barbs and shaft).External contamination seems to have influence on the levels found in feathers for some compounds. However, it does not seem to be the only cause of the high levels observed in barbs in comparison with those detected in shaft.Feather could be considered as a promising non-destructive tool for organochlorine pesticides in birds. Future studies should be carried out to obtain correlations between concentrations in feathers and internal tissues of birds. Moreover, it is necessary to evaluate additional factors such as age, sex and nutritional status of the birds in order to check its effect on the OC levels in feathers(AU)

Persistence of unsafe practice in everyday work: an exploration of organizational and psychological factors constraining safety in the operating room.

This paper explores the factors that influence the persistence of unsafe practice in an interprofessional team setting in health care, towards the development of a descriptive theoretical model for analyzing problematic practice routines. Using data collected during a mixed method interview study of 28 members of an operating room team, participants' approaches to unsafe practice were analyzed using the following three theoretical models from organizational and cognitive psychology: Reason's theory of "vulnerable system syndrome", Tucker and Edmondson's concept of first and second order problem solving, and Amalberti's model of practice migration. These three theoretical approaches provide a critical insight into key trends in the interview data, including team members' definition of error as the breaching of standards of practice, nurses' sense of scope of practice as a constraint on their reporting behaviours, and participants' reports of the forces influencing tacit agreements to work around safety regulations. However, the relational factors underlying unsafe practice routines are poorly accounted for in these theoretical approaches. Incorporating an additional theoretical construct such as "relational coordination" to account for the emotional human features of team practice would provide a more comprehensive theoretical approach for use in exploring unsafe practice routines and the forces that sustain them in healthcare team settings.

Getting teams to talk: development and pilot implementation of a checklist to promote interprofessional communication in the OR.

BACKGROUND: Pilot studies of complex interventions such as a team checklist are an essential precursor to evaluating how these interventions affect quality and safety of care. We conducted a pilot implementation of a preoperative team communication checklist. The objectives of the study were to assess the feasibility of the checklist (that is, team members' willingness and ability to incorporate it into their work processes); to describe how the checklist tool was used by operating room (OR) teams; and to describe perceived functions of the checklist discussions. METHODS: A checklist prototype was developed and OR team members were asked to implement it before 18 surgical procedures. A research assistant was present to prompt the participants, if necessary, to initiate each checklist discussion. Trained observers recorded ethnographic field notes and 11 brief feedback interviews were conducted. Observation and interview data were analyzed for trends. RESULTS: The checklist was implemented by the OR team in all 18 study cases. The rate of team participation was 100% (33 vascular surgery team members). The checklist discussions lasted 1-6 minutes (mean 3.5) and most commonly took place in the OR before the patient's arrival. Perceived functions of the checklist discussions included provision of detailed case related information, confirmation of details, articulation of concerns or ambiguities, team building, education, and decision making. Participants consistently valued the checklist discussions. The most significant barrier to undertaking the team checklist was variability in team members' preoperative workflow patterns, which sometimes presented a challenge to bringing the entire team together. CONCLUSIONS: The preoperative team checklist shows promise as a feasible and efficient tool that promotes information exchange and team cohesion. Further research is needed to determine the sustainability and generalizability of the checklist intervention, to fully integrate the checklist routine into workflow patterns, and to measure its impact on patient safety.

Communication failures in the operating room: an observational classification of recurrent types and effects.

BACKGROUND: Ineffective team communication is frequently at the root of medical error. The objective of this study was to describe the characteristics of communication failures in the operating room (OR) and to classify their effects. This study was part of a larger project to develop a team checklist to improve communication in the OR. METHODS: Trained observers recorded 90 hours of observation during 48 surgical procedures. Ninety four team members participated from anesthesia (16 staff, 6 fellows, 3 residents), surgery (14 staff, 8 fellows, 13 residents, 3 clerks), and nursing (31 staff). Field notes recording procedurally relevant communication events were analysed using a framework which considered the content, audience, purpose, and occasion of a communication exchange. A communication failure was defined as an event that was flawed in one or more of these dimensions. RESULTS: 421 communication events were noted, of which 129 were categorized as communication failures. Failure types included "occasion" (45.7% of instances) where timing was poor; "content" (35.7%) where information was missing or inaccurate, "purpose" (24.0%) where issues were not resolved, and "audience" (20.9%) where key individuals were excluded. 36.4% of failures resulted in visible effects on system processes including inefficiency, team tension, resource waste, workaround, delay, patient inconvenience and procedural error. CONCLUSION: Communication failures in the OR exhibited a common set of problems. They occurred in approximately 30% of team exchanges and a third of these resulted in effects which jeopardized patient safety by increasing cognitive load, interrupting routine, and increasing tension in the OR.

Fungi associated with food and feed commodities from Ecuador.

Freshly harvested soybean, rice and corn from farms and corn-based pelleted feeds were collected from ranches from the coastal and mountain regions in Ecuador during 1998, and assessed for fungal contamination. The most prevalent fungi on pelleted feed were Aspergillus flavus and Fusarium graminearum. The prevalent fungi recovered from soybean were F. verticillioides, F. semitectum, Aspergillus flavus and A. ochraceus. In rice, F. oxysporum was the most prevalent toxigenic fungal species recorded, followed by F. verticillioides and A. flavus. In corn, F. verticillioides was the most prevalent fungus isolated in both the coastal and mountain regions, with high isolation frequencies of A. flavus and A. parasiticus at the coast. Based on the toxigenic species recovered, ochratoxin A may pose a contamination risk for soybean. A higher probability of aflatoxin contamination of corn was found in the coastal samples compared to those of the mountain region, while a risk of fumonisin contamination of corn exists in both regions.

Forming professional identities on the health care team: discursive constructions of the 'other' in the operating room.

BACKGROUND: Inter-professional health care teams represent the nucleus of both patient care and the clinical education of novices. Both activities depend upon the'talk' that team members use to interact with one another. This study explored team members' interpretations of tense team communications in the operating room (OR). METHODS: The study was conducted using 52 team members divided into 14 focus groups. Team members comprised 13 surgeons, 19 nurses, nine anaesthetists and 11 trainees. Both uni-disciplinary (n = 11) and multi-disciplinary (n = 3) formats were employed. All groups discussed three communication scenarios, derived from prior ethnographic research. Discussions were audio-recorded and transcribed. Using a grounded theory approach, three researchers individually analysed sample transcripts, after which group discussions were held to resolve discrepancies and confirm a coding structure. Using the confirmed code, the complete data set was coded using the 'NVivo' qualitative data analysis software program. RESULTS: There were substantial differences in surgeons', nurses', anaesthetists', and trainees' interpretations of the communication scenarios. Interpretations were accompanied by subjects' depictions of disciplinary roles on the team. Subjects' constructions of other professions' roles, values and motivations were often dissonant with those professions' constructions of themselves. CONCLUSIONS: Team members, particularly novices, tend to simplify and distort others' roles and motivations as they interpret tense communication. We suggest that such simplifications may be rhetorical, reflecting professional rivalries on the OR team. In addition, we theorise that novices' echoing of role simplification has implications for their professional identity formation.

Time as a catalyst for tension in nurse-surgeon communication.

Carefully studying communication patterns between nurses and surgeons questions popular stereotypes about OR discourse and expands educators' understanding of the factors that motivate team communication, patterns that are habitual among team members, and issues that act as catalysts for tension. This study examines the nature of communication between perioperative nurses and surgeons and identifies patterns and sites of tension. Researchers observed 128 hours of interaction between nurses and surgeons in four surgical divisions at one teaching hospital in Ontario, Canada. Field notes were read, coded, and analyzed independently. Results showed that higher tension in nurse-surgeon communication clusters around particular themes, the most dominant of which is time. Analysis of this theme reveals communication strategies that allow surgeons and nurses to achieve individual goals and support social cohesion among team members.

Identification of three novel mutations in the MYO7A gene

Three new mutations in the myosin VIIA gene involved in the pathogenesis of Usher syndrome type Ib are reported. These mutations are K1080X in exon 25, E1170K in exon 28, and Y1719C in exon 37. It is presumed that these mutations are involved in the Usher syndrome Ib phenotype. Hum Mutat 14:181, 1999. Copyright 1999 Wiley-Liss, Inc.

The registered nurse surgical assistant.

This article provides a candid account of the evolution of two Registered Nurse Surgical Assistant positions, and their roles as members of a large open-heart team. A description of the expectation of the job, the role in surgery and the challenges and rewards are outlined.

Continuous quality improvement: a perioperative nursing approach.

This article describes in detail the steps taken in the Operating Room at the Toronto Hospital in implementing a Continuous Quality Management framework. Focusing on active participation, all levels of the O.R. staff worked through a process of identifying, developing, measuring and evaluating standards of practice. A step by step framework was followed in order to monitor current standards and develop new ones, thereby complying with the institution's mandate of supporting Continuous Quality Improvement.