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Are branched chain fatty acids the natural substrates for P450(BM3)?
Cryle, Max J; Espinoza, Rocio D; Smith, Sarah J; Matovic, Nicholas J; De Voss, James J.
Chem Commun (Camb) ; (22): 2353-5, 2006 Jun 14.
Article in English | MEDLINE | ID: mdl-16733577

ABSTRACT

Branched chain fatty acids are substrates for cytochrome P450(BM3) (CYP102) from Bacillus megaterium; oxidation of C15 and C17 iso and anteiso fatty acids by P450(BM3) leads to the formation of hydroxylated products that possess high levels of regiochemical and stereochemical purity.


Subject(s)
Bacterial Proteins/chemistry , Cytochrome P-450 Enzyme System/chemistry , Fatty Acids/chemistry , Mixed Function Oxygenases/chemistry , Bacillus megaterium/enzymology , Catalysis , Fatty Acids/chemical synthesis , Hydroxylation , Molecular Structure , NADPH-Ferrihemoprotein Reductase , Oxidation-Reduction , Stereoisomerism , Substrate Specificity
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