1.
J Forensic Sci
; 43(2): 410-2, 1998 Mar.
Article
in English
| MEDLINE
| ID: mdl-9544554
ABSTRACT
The DRPLA CAG repeats polymorphism has been studied in an Italian population sample. PCR amplification, manual PAGE and silver staining were employed. A total of 16 different alleles, spanning the range from 5 to 21 CAG triplettes, was observed. The heterozygosity was 0.81 and no significant deviation from Hardy-Weinberg equilibrium was found 81 meioses from parentage testing were also analyzed and a Mendelian pattern of inheritance was observed in all cases. In addition, we could successfully type DRPLA locus in some forensic specimens, 1 ng of DNA allowing clear definition of alleles. The authors conclude that the DRPLA CAG repeats analysis may be useful for forensic applications.
Subject(s)
DNA/analysis , Nerve Tissue Proteins/genetics , Neurodegenerative Diseases/genetics , Polymorphism, Genetic , Trinucleotide Repeats/genetics , Alleles , DNA Fingerprinting/methods , DNA Primers/chemistry , Female , Humans , Italy , Male , Polymerase Chain Reaction
2.
Health Phys
; 49(6): 1295-8, 1985 Dec.
Article
in English
| MEDLINE
| ID: mdl-4077535