ABSTRACT
The rapid chemical analysis of individual cells is an analytical capability that will profoundly impact many fields including bioaerosol detection for biodefense and cellular diagnostics for clinical medicine. This article describes a mass spectrometry-based analytical technique for the real-time and reagentless characterization of individual airborne cells without sample preparation. We characterize the mass spectral signature of individual Bacillus spores and demonstrate the ability to distinguish two Bacillus spore species, B. thuringiensis and B.atrophaeus, from one another very accurately and from the other biological and nonbiological background materials tested with no false positives at a sensitivity of 92%. This example demonstrates that the chemical differences between these two Bacillus spore species are consistently and easily detected within single cells in seconds.
Subject(s)
Aerosols/analysis , Air Microbiology , Spores, Bacterial/isolation & purification , Bacillus/chemistry , Bacillus/isolation & purification , Bacillus thuringiensis/chemistry , Bacillus thuringiensis/isolation & purification , Clostridium/chemistry , Clostridium/isolation & purification , Complex Mixtures/analysis , Culture Media/pharmacology , Mass Spectrometry/methods , Reproducibility of Results , Spores, Bacterial/classification , Spores, Bacterial/drug effects , Spores, Fungal/chemistry , Spores, Fungal/classification , Spores, Fungal/isolation & purificationABSTRACT
Realizamos un dobel estudio del estado nutricional de 1027 niños, valorandolos mediante el método Edad-Peso-Talla y comparándolo con la valoración obtenida por el Método del Brazalete de la Salud que se introduce a través de UNICEF. Encontramos un grado de acierto en 67.5% y un error de 31.5% y debemos destacar en su totalidad a los niños como Desnutridos cuando según la valoración por edad-Peso-Talla son normales. En ningún caso se da el error de considerar Normal a un niño Desnutrido
