ABSTRACT
Natural killer (NK) cell activity in peripheral blood mononuclear cells (PBMCs) from patients with Hodgkin's disease was studied using 4 h 51Cr release assay and K562 cells as sensitive targets. PBMCs were obtained from 15 previously untreated patients at different stages of their disease. PBMCs were also obtained from 46 patients treated by radiation therapy or combined chemotherapy and radiation therapy. Twenty healthy age-matched volunteer donors were used as controls to the treated patients. For these normal donors the mean cytotoxicity was 24.8 +/- 5.67% at a 100:1 effector-target cell ratio; and 43.7 +/- 12.1% for the treated cancer patients. Fifteen healthy age-matched volunteer donors were used as controls to the untreated patients. The mean cytotoxicity for these normal donors was 20.8 +/- 3.61% at a 100:1 effector-target cell ratio; and 37.6 +/- 6.65% for the previously untreated cancer patients. The mean cytotoxicity for all 35 normal donors was 23.1 +/- 5.22% at a 100:1 effector-target cell ratio. Most treated patients (93.5%) had a complete response to therapy and a significant difference was found between the mean cytotoxicity of the whole group (46 treated patients), compared with controls (P < 0.001). A significant difference (P < 0.05) was also observed when the same 11 patients were studied before and after treatment.
Subject(s)
Antineoplastic Agents/adverse effects , Hodgkin Disease/therapy , Killer Cells, Natural/radiation effects , Adult , Aged , Case-Control Studies , Combined Modality Therapy , Hodgkin Disease/immunology , Humans , Killer Cells, Natural/drug effects , Middle Aged , Radiotherapy/adverse effects , Treatment OutcomeABSTRACT
Impairment of natural cytotoxicity mediated by natural killer (NK) cells may play a role in the pathogenesis of penile carcinoma. The aim of this study was to examine the NK activity profile and its prognostic significance in patients with squamous cell carcinoma of the penis. The NK activity was measured in peripheral blood mononuclear cells (PBMCs) from 39 patients diagnosed histologically as having invasive squamous cell penile carcinoma and 4 patients with verrucous carcinoma of the penis. Of 39 patients with invasive squamous cell carcinoma, 4 had undergone previous penile amputation. According to the prognosis, the patients with invasive squamous cell carcinoma were divided into two groups: with metastasis and without metastasis. The patients were evaluated in relation to clinicopathologic variables using univariate analyses. NK cell activity was significantly decreased in all patients with penile carcinoma when compared with the control groups (p < 0.0001). There was no statistically significant difference between the groups with and without metastasis. We conclude that there is a decrease in NK activity in PBMCs from patients with penile carcinoma and that the presence of advanced disease or metastatic involvement is not responsible for this reduction.
Subject(s)
Carcinoma, Squamous Cell/immunology , Cytotoxicity, Immunologic , Killer Cells, Natural/immunology , Penile Neoplasms/immunology , Adult , Aged , Aged, 80 and over , Carcinoma, Squamous Cell/secondary , Carcinoma, Verrucous/immunology , Carcinoma, Verrucous/secondary , Case-Control Studies , Humans , In Vitro Techniques , K562 Cells , Male , Middle Aged , Neoplasm Invasiveness/immunology , Neoplasm Staging , PrognosisABSTRACT
Natural killer (NK) cell activity in peripheral blood mononuclear cells (PBMCs) from women with carcinoma of the uterine cervix was studied using a 4-hour 51Cr release assay and K562 cells as the sensitive target. PBMCs were obtained from 21 previously untreated patients at different stages of disease according to the International Federation of Gynecology and Obstetrics classification. PBMCs were also obtained from 36 patients treated with radiation therapy at different disease stages. Seventeen healthy age-matched volunteer women were used as controls. Mean cytotoxicity for the normal donors was 25.1 +/- 6.56% at a 100:1 effector-target cell ratio, 33.8 +/- 7.96% for the previously untreated cancer patients and 52 +/- 18.4% for the treated cancer patients. Most of the treated patients (86%) showed a complete response to radiation therapy and the mean cytotoxicity of the whole group (36 treated patients) was significantly increased compared to controls (p < 0.05). It is suggested that radiation therapy may produce cell alterations leading to an increase in NK cell activity in patients treated for uterine cervical cancer. The significance of this increase is discussed.
