Monoclonal antibodies recognize 2300 years aged alkaline phosphatase.

It was attempted to monitor the immunological response of monoclonal antibodies directed to human alkaline phosphatase in ancient Egyptian bones from the ptolemeic period. The intactness of the respective epitopes of the bone enzyme was successfully demonstrated in an ELISA. Fortunately, the mummified bone was not contaminated by fungi and bacteria due to the fungicidal and bactericidal reactivity of the ancient pretreatment employing resins of pistachio for mummification. The enzyme was enriched using gel chromatography, anion exchange and affinity chromatography to yield 310 +/- 7 mU/mg. The enzymically active fractions of the wheat-germ lectin affinity chromatography were subjected to ELISA. The best binding affinity was detected using the monoclonal antibody BAP A while the reactions of all the other four antibodies BAP B, BAP G, BAP 4A5 and BAP 5D4 were substantially diminished.

Enzymatic and immunological activity of 4000 years aged bone alkaline phosphatase.

Structurally intact and functionally active human bone alkaline phosphatase was isolated from clavicle fragments of IDU, an Egyptian mummy of the Old Kingdom (2150 +/- 50 BC). Both anion exchange and affinity chromatographies were employed to optimise the preparation of the ancient enzyme resulting in a specific activity of 180 +/- 30 mU/mg. The intactness of the bone enzyme fractions of the wheat-germ lectin affinity chromatography was successfully demonstrated in an ELISA using the monoclonal antibody BAP A. Fortunately, the mummified bone was not contaminated by fungi or bacteria.

Zn2Mg alkaline phosphatase in an early ptolemeic mummy.

Bone samples of a ptolemeic mummy have been employed to study the mode of conservation on the intactness of Zn2Mg alkaline phosphatase in both structure and catalytic activity. A protein of M(r) = 190 +/- 10 kDa being identical to the 200 kDa enzyme of fresh human bones was successfully isolated. Regardless of age 200 kDa protein bands and a distinct subunit at 60 kDa were seen in SDS-PAGE electrophoresis. The 200 kDa band was also monitored by activity staining. The specific activity was 120 mU/mg and 65% of the respective activity obtained in the identical preparation using fresh human tibia or rib. The enzymic activity was inhibited in the presence of 1,10-phenanthroline and L-homoarginine. Radiocarbon dating supported the assignment of the mummy to the early ptolemeic period. Among the many bactericidal and fungicidal components employed for mummification were aromatic alcohols, mono- and sesquiterpenes. Pistachio resin was the major balm resin used. The microbiological sterility of the bone surface was ascertained by independent bacterial and fungal examinations.

[Analysis and epidemiology of gallstones in deceased and autopsied patients of the Thurgau canton]. / Analyse und Epidemiologie von Gallensteinen anhand im Kanton Thurgau verstorbener und obduzierter Patienten.

In our postmortem examinations of patients in the canton of Thurgau from May 1989 through December 1991 we found a cholelithiasis or a condition after a cholecystectomy of gallstones in 44% of the women and 23% of the men. While gallstones were found in only 11% of the women and 7% of the men up to age 50, half of all women and every third man in the age group over 90 had gallstones. Among the gallstone carriers, 8% of the women and 2.5% of the men had developed a gall bladder--gall duct carcinoma. Where a liver cirrhosis was present, gallstones occurred at nearly the same rate in both sexes (45%/44%). 33% of the persons with gallstones had a solitary stone, and 31% had more than 10 stones. More than a third of all stones had a cholesterol content of more than 80%, and every seventh stone had a bilirubin content higher than 80%. The calcium content of more than 40% of the stones exceeded 5%. The examination for bilirubin can be easily performed with some exercise by means of the bilirubin test field on the urine sticks supplied by the Boehringer Mannheim company.