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1.
BMC Complement Altern Med ; 17(1): 367, 2017 Jul 17.
Article in English | MEDLINE | ID: mdl-28716085

ABSTRACT

BACKGROUND: Curcuma longa L. is a well-known medicinal plant that has been used for its anti-cancer, neuroprotective, and hepatoprotective effects. However, the neuroprotective effect of fermented C. longa (FCL) has not been reported. Therefore, in this study, the effectiveness of FCL for the regulation of memory dysfunction was investigated in two brain cell lines (rat glioma C6 and murine microglia BV2) and scopolamine-treated mice. METHODS: C. longa powder was fermented by 5% Lactobacillus plantarum K154 containing 2% (w/v) yeast extract at 30 °C for 72 h followed by sterilization at 121 °C for 15 min. The protective effects of fermented C. longa (FCL) on oxidative stress induced cell death were analyzed by MTT assay in C6 cells. The anti-inflammatory effects of FCL were investigated by measuring the production of nitric oxide (NO) and prostaglandin E2 (PGE2) as well as the expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated BV2 cells. The step-through passive avoidance test, Morris water maze test, acetylcholinesterase (AChE) activity, and expression of cAMP response element-binding protein (CREB) and brain-derived neurotropic factor (BDNF) were employed to determine the effects of FCL on scopolamine-induced memory deficit in mice. The contents of curcuminoids were analyzed through LC/MS. RESULTS: Pretreatment with FCL effectively prevented the cell death induced by oxidative stress in C6 cells. Moreover, FCL inhibited the production NO and PGE2 via the inhibition of iNOS and COX-2 expression in BV2 cells. FCL significantly attenuated scopolamine-induced memory impairment in mice and prevented scopolamine-induced AChE activity in the hippocampus. Additionally, FCL reversed the reduction of CREB and BDNF expression. The curcuminoids content in FCL was 1.44%. CONCLUSION: FCL pretreatment could alleviate scopolamine-induced memory impairment in mice, as well as oxidative stress and inflammation in C6 and BV2 cells, respectively. Thus, FCL might be a useful material for preventing impairment of learning and memory.


Subject(s)
Amnesia/drug therapy , Brain/drug effects , Curcuma/chemistry , Inflammation/drug therapy , Neuroprotective Agents/therapeutic use , Oxidative Stress/drug effects , Phytotherapy , Acetylcholinesterase/metabolism , Amnesia/chemically induced , Amnesia/metabolism , Animals , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/therapeutic use , Antioxidants/pharmacology , Antioxidants/therapeutic use , Brain/metabolism , Brain-Derived Neurotrophic Factor/metabolism , Cell Line , Curcumin/analysis , Curcumin/pharmacology , Curcumin/therapeutic use , Cyclic AMP Response Element-Binding Protein/metabolism , Fermentation , Inflammation/chemically induced , Inflammation/metabolism , Inflammation Mediators/metabolism , Lipopolysaccharides , Male , Memory Disorders , Mice, Inbred ICR , Neuroprotective Agents/pharmacology , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Rats , Scopolamine
2.
Biosci Biotechnol Biochem ; 79(5): 700-6, 2015.
Article in English | MEDLINE | ID: mdl-25559019

ABSTRACT

Methanol extract of Zizania latifolia was partitioned with EtOAc, n-BuOH, and H2O. From the EtOAc layers, a new flavonolignan along with a known flavone and three known flavonolignans, tricin (1), salcolin A (2), salcolin B (3), and salcolin C (4), were isolated through repeated silica gel and ODS column chromatography. The chemical structure of the new flavonolignan was determined to be tricin-4'-O-[erythro-ß-guaiacyl-(7″-O-methyl)-glyceryl] ether and was named salcolin D (5) based on physicochemical and spectroscopic data, including FT-NMR and ESI-MS. All compounds were isolated for the first time from this plant. Compounds 2-5, tricin derivatives, all exhibited higher anti-inflammatory and anti-allergy activities than tricin. In particular, salcolin D (5) was shown to have the strongest inhibitory activity against LPS-induced NO production in RAW 264.7 cells as well as ß-hexosaminidase release in IgE-sensitized RBL-2H3 cells. These results suggest that the presence of tricin derivatives conveys allergy and inflammation treatment ability to Z. latifolia.


Subject(s)
Anti-Allergic Agents/pharmacology , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Flavonoids/chemistry , Poaceae/chemistry , Animals , Anti-Allergic Agents/chemistry , Anti-Inflammatory Agents, Non-Steroidal/chemistry , Cell Line/drug effects , Drug Evaluation, Preclinical/methods , Flavones/chemistry , Flavones/pharmacology , Flavonoids/pharmacology , Flavonolignans/chemistry , Flavonolignans/isolation & purification , Flavonolignans/pharmacology , Immunoglobulin E/pharmacology , Lignans/chemistry , Lignans/pharmacology , Lipopolysaccharides/pharmacology , Mice , Molecular Structure , Nitric Oxide/metabolism , Plant Components, Aerial/chemistry , Plant Extracts/chemistry , Plant Extracts/pharmacology , Rats , beta-N-Acetylhexosaminidases/antagonists & inhibitors , beta-N-Acetylhexosaminidases/metabolism
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