ABSTRACT
In experiments on (CBA X C57B1)F1 mice a study was made of the radioprotective efficiency of some hypotensive drugs. Hemiton administered in different ways, within a wide dose range and at different times before irradiation was shown to possess a pronounced protective action. The substitution of the acid residue in hemiton decreased its activity.
Subject(s)
Antihypertensive Agents/pharmacology , Radiation-Protective Agents/pharmacology , Animals , Clonidine/pharmacology , Dose-Response Relationship, Drug , Gamma Rays , Male , Methyldopa/pharmacology , Mice , Mice, Inbred C57BL , Mice, Inbred CBAABSTRACT
Interaction of bonive serum albumin with copolymers 2-methyl-5-vinylpyridine, acrylic and methacrylic acids in different proportions in aqueous solutions was studied. It was shown, that in a wide range of ration of the components soluble cooperative complexes were formed. Ability of amphotheric copolymers to form complexes with bovine serum albumin depend both on the composition of the copolymers and on the pH of the medium. The non-polar interactions of the protein globules with hydrocarbonic 2-methyl-5-vinylpyridine chain fragments of the polyampholyte play an important role in the association of similarly charged particals. It was shown that besides this effect of chain fragments of 2-methyl-5-vinylpyridine a significant contribution in the association is made by non-polar interactions, created by methyl groups in copolymer 2-methyl-5-vinylpyridine and methacrylic acid. The association mechanism and structure of the formed complexes was studied. It was shown that complex formation is accompanied by non-equal distribution of the chain copolymer between the protein globules and appearance of asymmetric particles of the complex. A correlation between the ability of polyampholytes to bind protein and their physiological action was found.
Subject(s)
Acrylates , Methacrylates , Polyvinyls , Serum Albumin, Bovine , Kinetics , Microscopy, Electron , Polymers , Protein Binding , Solubility , Structure-Activity RelationshipABSTRACT
It has been shown by high-velocity sedimentation that BSA forms soluble complexes with polyacrylic acid (PAA) and sodium polystyrene sulfonate (PSSNa) with neutral water solutions. Factors responsible for polyanion binding of similarly charged BSA molecules are analysed. It has been found that occupation of PAA and PSSNa chains with BSA globules proceeds steadily. BSA-PAA and BSA-PSSNa complexes are compared with the earlier described products of BSA sorption on poly-4-vinyl-N-ethylpyridine bromide, where the occupation of polycations with protein globules proceeds unevenly. Reasons for the existence of two types of soluble BSA-polyelectrolyte complexes are described; probable structural model of BSA complex with polyacids and polyanions is suggested.
Subject(s)
Acrylic Resins , Polystyrenes , Serum Albumin, Bovine , Chemical Phenomena , Chemistry , Polymethacrylic Acids , Solubility , Sulfonic Acids , UltracentrifugationABSTRACT
Interaction of bovine serum albumin (BSA) with quaternized poly-4-vinyl pyridine (PE) in aqueous solutions at pH 7 was studied. It was shown that in a wide range of the ratios of the components (nBSA/nPE) soluble stable cooperative complexes were formed. At the same time a certain critical content of the protein exists at which the system loses its homogeneity. Complex formation is not accompanied by protein denaturation. At smaller nBSA/nPE ratios non-homogeneous distribution of protein globulas among polyelectrolite macromolecules was found; this corresponded to the "all or none" principle. Using ultracentrifugation technique viscosimetric measurements and electron microscopy it was shown that the soluble complexes exist in the form of rode-like particles consisting of protein globules stabilized by polycation chains. Such particle can be considered as a model of nucleoprotein complex. At certain crytical nBSA/nPE rations the rod-like particles aggregate with additional number of BSA-molecules and form more complicate soluble and insoluble cooperative complexes. Possible structural models of the complexes described were suggested and the thermodinamic and kinetic cryteria of their self-assembly were discussed.
Subject(s)
Polyvinyls , Serum Albumin, Bovine , Drug Stability , Hydrogen-Ion Concentration , Kinetics , Microscopy, Electron , Models, Biological , Nucleoproteins , Protein Binding , Protein Conformation , ViscositySubject(s)
Haptens , Picrates/immunology , Pyridines/immunology , Vinyl Compounds/immunology , Anaphylaxis/etiology , Animals , Guinea Pigs , Mice , Mice, Inbred C57BL , Mice, Inbred CBAABSTRACT
We investigated the effect of polyacrilic acid (PAA) on the immune response in mice of various strains on sheep red blood cells and also the influence of poly-2-methyl-5-vinyl-pyridine (PMVY), PAA and their statistical copolymers on antibody-forming cells (AFC) production in cultures of T- and B-lymphocytes in vivo. PAA was seen to increase accumulation of AFC in the spleen of mice depending on their genotypes. PMVP and PAA were found to intensify the cooperating interaction of T- and B-lymphocytes, whereas their copolymers exert quite an opposite effect. The injection of copolymers to the recipients of cooperating T- and B-lymphocytes practically results in the complete elimination of the cooperation effect between T- and B-lymphocytes in the immune response to sheep erythrocytes without cytostatic action of cell proliferation.
Subject(s)
Acrylic Resins/pharmacology , Antibody Formation/drug effects , Lymphocytes/drug effects , Polyvinyls/pharmacology , Pyridines/pharmacology , Animals , B-Lymphocytes/drug effects , Cells, Cultured , Genotype , Hybridization, Genetic , Mice , Mice, Inbred A , Mice, Inbred C3H , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Inbred Strains , Species Specificity , T-Lymphocytes/drug effectsABSTRACT
The influence of poly-4-vinylpiridine (P-4-VP) on the production of antibody forming cells (AFC) has been studied. P-4-VP induces helper activity in lymphoid organs of B-mice. P-4-VP injected into mice substitutes T cell function in the development of immunological memory. The possibility of substitution by P-4-VP of various functions or of different subpopulations of T cells in T cell-deprived mice is discussed.
Subject(s)
Immunity/drug effects , Polyvinylpyridine N-Oxide/immunology , Polyvinyls/immunology , T-Lymphocytes/immunology , Animals , Antibody-Producing Cells/drug effects , Antibody-Producing Cells/radiation effects , Bone Marrow/drug effects , Bone Marrow Cells , Immunologic Memory/drug effects , Lymph Nodes/drug effects , Lymphoid Tissue/drug effects , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Radiation Effects , Spleen/drug effects , T-Lymphocytes/drug effects , Thymectomy , Thymus Gland/radiation effectsABSTRACT
The effect of poly-4-vinylpyridine and polyacrilic acid on production of antibodyforming cells to the sheep red blood cells in in vivo mixed cultures of T- and B-lymphocytes involved an obvious increase of the immune response. To a lesser extent theimmune response was increased in B-lymphocyte cultures, and no such effect was observed in T-lymphocyte cultures. The poly-4-vinlpyridine and polyacrilic acid were concluded to enhance cooperation between T- and B-lymphocytes and, probably, to replacethe T-lymphocytes function in B-mice.
