ABSTRACT
AIM: The study of effectiveness of intravenous immunoglobulin (IVIG) in therapy of idiopathic thrombocytopenic purpura (ITP) in adults. MATERIALS AND METHODS: High doses of IVIG (0.2-0.4 g/kg b.w. for 4-6 days) were given to 6 female patients aged 20 to 59 years (median--39 years) with immune thrombocytopenia. 4 patients had primary ITP resistant to glucocorticosteroids (GCS) and 1 female had chronic ITP treated by splenectomy without effect. 1 patient with rheumatoid arthritis developed severe thrombocytopenia combined with agranulocytosis when treated with nonsteroid antiinflammatory drugs. RESULTS: The response was observed in 5 of 6 patients (in 4 patients resistant to GCS and 1 RA patient). In 3 of them the effect was rated as excellent (platelets level > 150,000 per cubic millimeter), in 2 patients it was good (platelets count from 50,000 to 150,000 per cubic millimeter). Splenectomy was performed in 4 cases with ITP on day 8-14 after IVIG therapy. The 2- and 6-month follow-up evidenced for good results of the surgical treatment. The RA patient showed a stable rise of the blood count. Serious side effects of IVIG therapy were not registered. CONCLUSION: IVIG of Russian produce is effective in the treatment of drug-induced thrombocytopenia and ITP resistant to GCS.
Subject(s)
Immunoglobulins, Intravenous/therapeutic use , Purpura, Thrombocytopenic, Idiopathic/therapy , Adult , Female , Follow-Up Studies , Humans , Middle Aged , Platelet Count/drug effects , Purpura, Thrombocytopenic, Idiopathic/blood , Russia , Splenectomy , Treatment OutcomeABSTRACT
The methods of the radioimmunoassay and the blot hybridization of restricted fragments of chromosomal DNA have been used for the characterization of V. cholerae atypical strains isolated from the natural environment. For all strains under study, the radioimmunoassay has been found to yield the most sharply defined data characterizing their atoxigenicity. The absence of the structural genes of toxin in the chromosomes of these strains has been shown by the method of blot hybridization. Some methodological simplifications of blot hybridization, having no adverse effect on the sensitivity of this method, have been tested.
Subject(s)
DNA, Bacterial/analysis , Nucleic Acid Hybridization , Vibrio cholerae/metabolism , Bacterial Toxins/biosynthesis , RadioimmunoassayABSTRACT
The effect of UV-irradiation on Vibrio cholerae cells and its changes mediated by the plasmid R245 have been studied. Vibrio cholerae strains 569B and RV31 have been shown to be considerably more sensitive to lethal effect of UV-irradiation as compared with Escherichia coli and Salmonella typhimurium cells. Highly toxigenic strain 569B and practically atoxigenic strain RV31 have the same UV-sensitivity. Lethal effect of UV-irradiation on Vibrio cholerae cells is increased when the irradiated cells are plated on enriched media. UV-induction of mutations was not registered in plasmidless strains of Vibrio cholerae. Plasmid R245 increases UV-resistance of vibrio cells and makes them UV-mutable.
Subject(s)
Mutation , Plasmids , Ultraviolet Rays , Vibrio cholerae/genetics , Conjugation, Genetic , R Factors , Vibrio cholerae/physiology , Vibrio cholerae/radiation effectsABSTRACT
The recombinant plasmid RP4 omega elt carrying Escherichia coli heat-labile enterotoxin elt genes with 70-80% homology with genes vct of Vibrio cholerae has been constructed. We used this plasmid to determine localization of the cholerae toxin genes vct on the map of Vibrio cholerae cholerae. Two types of the donors were revealed in matings of 10 strains of V. cholerae cholerae 569B/RP4 omega elt with the polyauxotrophic recipients RV31 and RV175: some strains had enhanced frequency of mobilization of ilv-1 and lys-6 markers, the others--of trp-1. Our data suggest that structural vct genes are located within two regions of V. cholerae cholerae 569B chromosome: trp-1 and ilv-1--lys-6.
Subject(s)
Cholera Toxin/genetics , Genes, Bacterial , Genes , Plasmids , Vibrio cholerae/genetics , Chromosome Mapping , Enterotoxins/genetics , Escherichia coli/genetics , Genetic Markers , Recombination, Genetic , Sequence Homology, Nucleic AcidABSTRACT
The authors describe two women with a history of partial red-cell aplasia of the bone marrow without any remissions for 2 and 3 years, followed by the development of erythromyelosis. The latter lasted from 1 to 2 years, the total illness duration being 4 to 5 years. All the attempts to achieve remissions with the use of splenectomy, mono- and polychemotherapy with a purpose of immunosuppression ended in failure. Partial red-cell aplasia is viewed as preleukemia.
Subject(s)
Leukemia, Erythroblastic, Acute/etiology , Red-Cell Aplasia, Pure/complications , Adult , Chronic Disease , Combined Modality Therapy , Female , Humans , Leukemia, Erythroblastic, Acute/pathology , Leukemia, Erythroblastic, Acute/therapy , Middle Aged , Red-Cell Aplasia, Pure/pathology , Red-Cell Aplasia, Pure/therapy , Spleen/pathologyABSTRACT
Recombinant E. coli plasmids are known to be obtained from E. coli cells using the plasmids coding EcoR1 restriction endonuclease. These plasmids were shown to possess various chromosomal or plasmid genes. The paper presents data on the construction of conjugative recombinant plasmid pSA1002, capable of conjugate transfer into V. cholerae cells. The stable maintenance and inheritance of the plasmid in V. cholerae cells have been demonstrated as well as phenotypic expression of its genes, including EcoR1 restriction endonuclease genes. The possibility of recombinant plasmids formation in V. cholerae cells dependent on EcoR1 restriction endonuclease, coded by pSA1002, is discussed.
