1H and (13)C NMR of multilamellar dispersions of polyunsaturated (22:6) phospholipids.

The polyunsaturated fatty acid docosahexaenoic acid (DHA) makes up approximately 50% of the lipid chains in the retinal rod outer segment disk membranes and a large fraction of the lipid chains in the membranes of neuronal tissues. There is an extensive literature concerned with the dietary requirements for essential fatty acids and the importance of DHA to human health, but relatively little research has been done on the physical properties of this important molecule. Using (1)H and (13)C MAS NMR measurements of dispersions of 1-palmitoyl-2-docosahexaenoyl-phosphatidylcholine in excess phosphate buffer, we have unambiguously assigned most of the resonances in both the (1)H and (13)C NMR spectra. We were able to use cross-polarization spectroscopy to follow the transfer of polarization from specific (1)H nuclei not only to their directly bonded (13)C but also to those (13)C that are in close proximity, even though they are not directly bonded. Cross-peaks in two-dimensional cross-polarization spectra revealed a close association between the choline headgroup and at least part of the DHA chain but not with the palmitate chain. Finally, we examined the dynamics of the different parts of this lipid molecule, using rotating frame spin-lattice relaxation measurements, and found that methylene groups of both chains experience important motions with correlation times in the 10-micros range, with those for the palmitate chain being approximately 50% longer than those of the DHA chain. The choline headgroup and the chain terminal groups have significantly shorter correlation times, and that part of the dipolar interaction that is fluctuating at these correlation times is significantly smaller for these groups than it is for the palmitate and DHA chain methylenes.

Changes in optical properties of ex vivo rat prostate due to heating.

This study examines the effectiveness of a single, first-order Arrhenius process in accurately modelling the thermally induced changes in the optical properties, particularly the reduced scattering coefficient, mu(s)', and the absorption coefficient, mu(a), of ex vivo rat prostate. Recent work has shown that mu(s)' can increase as much as five-fold due to thermal coagulation, and the observed change in mu(s)' has been modelled well according to a first-order rate process in albumen. Conversely, optical property measurements conducted using pig liver suggest that this change in mu(s)' cannot suitably be described using a single rate parameter. In canine prostate, measurements have indicated that while the absorption coefficient varies with temperature, it does not do so according to first-order kinetics. A double integrating sphere system was used to measure the reflectance and transmittance of light at 810 nm through a thin sample of prostate. Using prostate samples collected from Sprague Dawley rats, optical properties were measured at a constant elevated temperature. Tissue samples were measured over the range 54-83 degrees C. The optical properties of the sample were determined through comparison with reflectance and transmittance values predicted by a Monte Carlo simulation of light propagation in turbid media. A first order Arrhenius model was applied to the observed change in mu(s)' and mu(a) to determine the rate process parameters for thermal coagulation. The measured rate coefficients were Ea = (7.18 +/- 1.74) x 10(4) J mol(-1) and Afreq = 3.14 x 10(8) s(-1) for mu(s)'. It was determined that the change in mu(s)' is well described by a single first-order rate process. Similar analysis performed on the changes in mu(a) due to increased temperatures yielded Ea = (1.01 +/- 0.35) x 10(5) J mol(-1) and Afreq = 8.92 x 10(12) s(-1). The results for mu(a) suggest that the Arrhenius model may be applicable to the changes in absorption.

Technology transfer for women entrepreneurs: issues for consideration.

PIP: This article discusses the effectiveness of technology transfers to women entrepreneurs in developing countries. Most women's enterprises share common characteristics: very small businesses, employment of women owners and maybe some family members, limited working capital, low profit margins, and flexible or part-time work. Many enterprises do not plan for growth. Women tend to diversify and use risk-avoidance strategies. Support for women's enterprises ignores the characteristics of women's enterprises. Support mechanisms could be offered that would perfect risk-spreading strategies and dynamic enterprise management through other means than growth. Many initiatives, since the 1970s, have transferred technologies to women. Technologies were applied to only a few domains and were viewed as appropriate based on their small size, low level of complexity, low cost, and environmental friendliness. Technology transfers may not be viewed by beneficiaries as the appropriate answer to needs. The bottleneck in transfers to women is not in the development of prototypes, but in the dissemination of technology that is sustainable, appropriate, and accessible. Key features for determining appropriateness include baseline studies, consumer linkages, and a repetitive process. Institutional factors may limit appropriateness. There is a need for long-term outputs, better links with users, training in use of the technology, grouping of women into larger units, and technology availability in quantities large enough to meet demand. Guidelines need to be developed that include appropriate content and training that ensures transfer of knowledge to practice.^ieng