Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Biomaterials ; 26(34): 7068-81, 2005 Dec.
Article in English | MEDLINE | ID: mdl-15979140

ABSTRACT

Patients with stable vitiligo can be helped surgically using transplantation of autologous cultured melanocytes, but there is a need for a culture methodology that is free from xenobiotic agents and for a simple way of delivering cultured melanocytes to the patient to achieve pigmentation with good wound healing. The aim of this study was to develop a chemically defined surface, suitable for the co-culture of melanocytes and keratinocytes which could be used in the future for the treatment vitiligo patients to achieve both restoration of pigmentation and good wound healing. Two keratinocyte growth media and two melanocyte growth media were compared; two of these were serum free. Cells were seeded on a range of chemically defined substrates (produced by plasma polymerisation of acrylic acid, allylamine or a mixture of these monomers) either as mono- or co-cultures. Melanocytes and keratinocytes attached and proliferated on both acid and amine substrates (without significant preferences), and co-cultures of cells proliferated more successfully than individual cultures. One media, M2, which is serum free, supported expansion of melanocytes and to a lesser extent keratinocytes on several plasma polymer substrates. In conclusion, these data indicate that a combination of a chemically defined substrate with M2 media allows serum-free co-culture of melanocytes and keratinocytes.


Subject(s)
Acrylates/chemistry , Allylamine/chemistry , Coculture Techniques/methods , Keratinocytes/cytology , Melanocytes/cytology , Skin, Artificial , Tissue Engineering/methods , Biocompatible Materials/chemistry , Cell Adhesion , Cell Proliferation , Cell Survival , Cells, Cultured , Culture Media, Serum-Free , Humans , Keratinocytes/physiology , Materials Testing , Melanocytes/physiology , Surface Properties , Vitiligo/surgery
SELECTION OF CITATIONS
SEARCH DETAIL
...