ABSTRACT
In conjunction with the global trend towards sustainable industry, this review provides a summary of the research endeavors and efforts made in the field of exploiting eggshells in the production of hydroxyapatite (HA). HA is one of the most used biomaterials and has attracted considerable attention over the years towards biomedical applications. As the traditional production of HA from calcium and phosphorus chemical precursors synthetically has bottlenecks of being expensive, complex, time consuming, and results in a low biocompatible product, natural resources have become an attractive alternative option to synthesize HA, with trace elements providing a higher performance. Eggshell, with a growing production annually, is potentially a promising natural resource for HA production. Many studies have used different wet chemical precipitation routes to produce HA with properties comparable to synthetic HA. Thus, this review provides an overview of the various routes that can be used to synthesize HA from eggshells. In this review, the synthesis of HA from eggshells via wet chemical precipitation methods is specifically discussed in term of synthesis parameters and properties of the synthesized HA. This review should aid in choosing the most suitable route for HA production with the optimum parameters for obtaining the desired properties to meet the requirements of biomedical applications such as tissue engineering.
ABSTRACT
PURPOSE: Today, bulk-fill composites are used as a single layer with a thickness of up to 4-5 mm. However, is proper polymerization achieved with this increased thickness? METHODS: This study was designed to investigate the effect of thickness on the degree of conversion (DC) (n = 6), the elution of monomers (n = 6), depth of cure (DoC) (n = 10) and cytotoxicity (n = 6) of the bulk-fill composites SDR Flow Plus (SDR), SonicFill2 SingleFill (SF) and ACTIVA Bioactive Restorative (ACT) in comparison to the conventional G-aenial Posterior (GC). Two-way analysis of variance (ANOVA) was used to assess the interaction between materials and surfaces, and one-way ANOVA and Tukey tests were used to compare the degree of conversion, monomer elution and cytotoxicity values (P < 0.05). RESULTS: The highest DC was found at the top surface of SDR, while the lowest DC was found at SF. The V2 mm/V0 mm DoC ratios of the composites except ACTs were appropriate according to the threshold. None of the composites were cytotoxic on day 1. CONCLUSION: In bulk-fill composites, DC decreased and monomer elution increased with increasing depth. The V4 mm/V0 mm ratios of all bulk-fill groups were not appropriate. Additionally, only ACTs had a cell viability of <70% on day 7.
