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1.
Biochim Biophys Acta ; 1492(2-3): 320-9, 2000 Jul 24.
Article in English | MEDLINE | ID: mdl-11004504

ABSTRACT

The human ABF-1 gene is expressed in activated B-cells and Epstein-Barr virus-immortalized lymphoblastoid cell lines. ABF-1 represents the only member belonging to the basic helix-loop-helix (bHLH) family of transcription factors whose expression pattern is restricted to B-cells. ABF-1 forms heterodimeric complexes with E2A to modulate gene transcription. We report the cloning and characterization of the human ABF-1 gene and the promoter region. The gene spans more than 3 kb and contains two exons. Exon 1 contains 274 bp of a 5'-untranslated sequence (UTR) while exon 2 contains 1097 bp of 3'-UTR. Promoter analysis of the 5'-flanking region revealed no apparent B-cell-restricted control elements within approximately 700 bp, but clearly demonstrated the presence of a functional minimal promoter residing immediately upstream of the transcription start site. Analysis of the region containing the minimal promoter activity identified no CCAAT or TATA sequence. Lastly, we have assigned the ABF-1 gene to human chromosome 8q21.1 using fluorescence in situ hybridization (FISH). The cloning of the human ABF-1 gene will facilitate further biochemical and genetic studies of its function in the regulation of B-cell differentiation.


Subject(s)
DNA-Binding Proteins/genetics , Promoter Regions, Genetic/genetics , Saccharomyces cerevisiae Proteins , Transcription Factors/genetics , Transcriptional Activation , Base Sequence , Chromosome Mapping , Chromosomes, Human, Pair 8 , DNA/analysis , Genome, Human , Genomic Library , Humans , Karyotyping , Molecular Sequence Data , Reverse Transcriptase Polymerase Chain Reaction , TATA Box , Tumor Cells, Cultured
3.
Rev Esp Anestesiol Reanim ; 44(2): 70-8, 1997 Feb.
Article in Spanish | MEDLINE | ID: mdl-9148359

ABSTRACT

Low molecular weight heparins are a group of drugs that have only recently been introduced in clinical practice. The are widely used for prophylaxis in thromboembolic disease and are being employed increasingly to treat established venous thrombosis. One way in which these drugs are often used is for prophylaxis in the perioperative period for patients at high risk of developing venous thromboembolism, and the anesthesiologist must therefore be familiar with the main aspects of this application. We review pharmacological characteristics of these drugs as well as the literature on low molecular weight heparins, stressing points of main interest to the anesthesiologist and intensive care recovery unit specialist, namely adverse effects (mainly bleeding) and the implications that use of low molecular weight heparin will have on choice of anesthetic (in particular the dilemma of whether to use local/regional anesthesia).


Subject(s)
Anticoagulants/therapeutic use , Fibrinolytic Agents/therapeutic use , Heparin, Low-Molecular-Weight/therapeutic use , Postoperative Complications/prevention & control , Thromboembolism/prevention & control , Anesthesia, Spinal/adverse effects , Anticoagulants/chemistry , Anticoagulants/pharmacology , Factor Xa Inhibitors , Female , Fibrinolytic Agents/chemistry , Fibrinolytic Agents/pharmacology , Hemorrhage/chemically induced , Hemorrhage/prevention & control , Heparin, Low-Molecular-Weight/antagonists & inhibitors , Heparin, Low-Molecular-Weight/chemistry , Heparin, Low-Molecular-Weight/pharmacology , Humans , Male , Postoperative Complications/chemically induced , Pregnancy , Pregnancy Complications, Hematologic/drug therapy , Protamines/pharmacology , Protamines/therapeutic use , Subarachnoid Hemorrhage/etiology , Subarachnoid Hemorrhage/prevention & control , Thrombin/antagonists & inhibitors
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