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1.
Front Immunol ; 12: 719979, 2021.
Article in English | MEDLINE | ID: mdl-34381461

ABSTRACT

Adipose tissue macrophages (ATMs) regulate homeostasis and contribute to the metabolically harmful chronic inflammation in obese individuals. While evident heterogeneity of resident ATMs has been described previously, their phenotype, developmental origin, and functionality remain inconsistent. We analyzed white adipose tissue (WAT) during homeostasis and diet interventions using comprehensive and unbiased single-cell mass cytometry and genetic lineage tracking models. We now provide a uniform definition of individual subsets of resident ATMs. We show that in lean mice, WAT co-harbors eight kinetically evolving CD206+ macrophage subpopulations (defined by TIM4, CD163, and MHC II) and two CD206- macrophage subpopulations. TIM4-CD163+, TIM4-CD163- and CD206- macrophage populations are largely bone marrow-derived, while the proliferating TIM4+CD163+ subpopulation is of embryonic origin. All macrophage subtypes are active in phagocytosis, endocytosis, and antigen processing in vitro, whereas TIM4+CD163+ cells are superior in scavenging in vivo. A high-fat diet induces massive infiltration of CD206- macrophages and selective down-regulation of MHC II on TIM4+ macrophages. These changes are reversed by dietary intervention. Thus, the developmental origin and environment jointly regulate the functional malleability of resident ATMs.


Subject(s)
Adipose Tissue, White/metabolism , Macrophages/metabolism , Proteome/metabolism , Proteomics , Single-Cell Analysis , Adipose Tissue/immunology , Adipose Tissue/metabolism , Adipose Tissue, White/immunology , Animals , Biomarkers , Cell Differentiation , Cell Plasticity/genetics , Cell Plasticity/immunology , Cellular Reprogramming , Computational Biology , Energy Metabolism , Immunohistochemistry , Immunophenotyping , Macrophages/immunology , Male , Mice , Mice, Knockout , Models, Animal , Obesity/etiology , Obesity/metabolism , Obesity/pathology , Phagocytosis , Proteomics/methods , Single-Cell Analysis/methods
2.
Mol Metab ; 31: 67-84, 2020 01.
Article in English | MEDLINE | ID: mdl-31918923

ABSTRACT

OBJECTIVE: Obesity is the result of positive energy balance. It can be caused by excessive energy consumption but also by decreased energy dissipation, which occurs under several conditions including when the development or activation of brown adipose tissue (BAT) is impaired. Here we evaluated whether iRhom2, the essential cofactor for the Tumour Necrosis Factor (TNF) sheddase ADAM17/TACE, plays a role in the pathophysiology of metabolic syndrome. METHODS: We challenged WT versus iRhom2 KO mice to positive energy balance by chronic exposure to a high fat diet and then compared their metabolic phenotypes. We also carried out ex vivo assays with primary and immortalized mouse brown adipocytes to establish the autonomy of the effect of loss of iRhom2 on thermogenesis and respiration. RESULTS: Deletion of iRhom2 protected mice from weight gain, dyslipidemia, adipose tissue inflammation, and hepatic steatosis and improved insulin sensitivity when challenged by a high fat diet. Crucially, the loss of iRhom2 promotes thermogenesis via BAT activation and beige adipocyte recruitment, enabling iRhom2 KO mice to dissipate excess energy more efficiently than WT animals. This effect on enhanced thermogenesis is cell-autonomous in brown adipocytes as iRhom2 KOs exhibit elevated UCP1 levels and increased mitochondrial proton leak. CONCLUSION: Our data suggest that iRhom2 is a negative regulator of thermogenesis and plays a role in the control of adipose tissue homeostasis during metabolic disease.


Subject(s)
Carrier Proteins/metabolism , Obesity/metabolism , Thermogenesis , Animals , Diet, High-Fat/adverse effects , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Obesity/chemically induced
3.
Nat Commun ; 10(1): 281, 2019 01 17.
Article in English | MEDLINE | ID: mdl-30655530

ABSTRACT

Macrophages serve multiple functions including immune regulation, morphogenesis, tissue homeostasis and healing reactions. The current paradigm holds that mammary gland macrophages first arise postnatally during the prepubertal period from the bone marrow-derived monocytes. Here we delineate the origins of tissue-resident mammary gland macrophages using high-dimension phenotypic analyses, cell-fate mapping experiments, gene-deficient mice lacking selective macrophage subtypes, and antibody-based depletion strategies. We show that tissue-resident macrophages are found in mammary glands already before birth, and that the yolk sac-derived and fetal liver-derived macrophages outnumber the adult-derived macrophages in the mammary gland also in the adulthood. In addition, fetal-derived mammary gland macrophages have a characteristic phenotype, display preferential periductal and perivascular localization, and are highly active in scavenging. These findings identify fetal-derived macrophages as the predominant leukocyte type in the adult mammary gland stroma, and reveal previously unknown complexity of macrophage biology in the breast.


Subject(s)
Cell Lineage/physiology , Macrophages/physiology , Mammary Glands, Animal/cytology , Monocytes/physiology , Morphogenesis/physiology , Animals , Cell Differentiation , Female , Fetus/cytology , Liver/cytology , Liver/growth & development , Male , Mice , Mice, Transgenic , Models, Animal , Yolk Sac/cytology , Yolk Sac/growth & development
4.
Cell Rep ; 21(3): 745-757, 2017 Oct 17.
Article in English | MEDLINE | ID: mdl-29045841

ABSTRACT

Cell surface metalloproteases coordinate signaling during development, tissue homeostasis, and disease. TACE (TNF-α-converting enzyme), is responsible for cleavage ("shedding") of membrane-tethered signaling molecules, including the cytokine TNF, and activating ligands of the EGFR. The trafficking of TACE within the secretory pathway requires its binding to iRhom2, which mediates the exit of TACE from the endoplasmic reticulum. An important, but mechanistically unclear, feature of TACE biology is its ability to be stimulated rapidly on the cell surface by numerous inflammatory and growth-promoting agents. Here, we report a role for iRhom2 in TACE stimulation on the cell surface. TACE shedding stimuli trigger MAP kinase-dependent phosphorylation of iRhom2 N-terminal cytoplasmic tail. This recruits 14-3-3 proteins, enforcing the dissociation of TACE from complexes with iRhom2, promoting the cleavage of TACE substrates. Our data reveal that iRhom2 controls multiple aspects of TACE biology, including stimulated shedding on the cell surface.


Subject(s)
ADAM17 Protein/metabolism , Carrier Proteins/metabolism , Proteolysis , 14-3-3 Proteins/metabolism , Animals , Carrier Proteins/chemistry , Cell Membrane/metabolism , Endoplasmic Reticulum/metabolism , HEK293 Cells , Humans , Mice, Knockout , Mitogen-Activated Protein Kinases/metabolism , Phosphorylation , Phosphoserine/metabolism , Signal Transduction , Substrate Specificity , Toll-Like Receptors/metabolism
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