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1.
Vet Pathol ; 47(5): 958-63, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20466857

ABSTRACT

The European brown hare (Lepus europaeus) plays an important role in the ecology of tularemia, and it may serve as a significant source of human infection. The aim of the present study was to examine the lesions induced by Francisella tularensis in 50 cases of naturally infected seropositive European brown hares. Gross pathological examination revealed scant to numerous grayish-white foci with diameters of 0.1 to 1.0 cm in single organs (24 cases) or multiple organs (20 cases) in 44 of 50 cases (88%). These lesions proved to be areas of granulomatous inflammation, frequently encompassing necrosis. F tularensis antigen was detected with immunohistochemistry in 46 of 50 cases (92%), whereas F tularensis ssp holarctica was isolated by culture and identified by polymerase chain reaction from 35 of 50 cases (70%). Infection by the respiratory route is suggested by the presence of the tissue lesions in thoracic organs of 44 of 50 cases (88%). These results emphasize the importance of the European brown hare as a reservoir of tularemia.


Subject(s)
Disease Reservoirs/veterinary , Francisella tularensis/isolation & purification , Hares/microbiology , Tularemia/veterinary , Zoonoses/microbiology , Animals , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Disease Reservoirs/microbiology , Female , Francisella tularensis/genetics , Immunohistochemistry/veterinary , Male , Polymerase Chain Reaction/veterinary , RNA, Ribosomal, 16S/chemistry , RNA, Ribosomal, 16S/genetics , Tularemia/microbiology , Tularemia/pathology
2.
Zoonoses Public Health ; 57(6): 417-22, 2010 Sep.
Article in English | MEDLINE | ID: mdl-19538455

ABSTRACT

Thirteen Francisella tularensis strains were isolated from 22 seropositive brown hares (Lepus europaeus) originating from different parts of Hungary, and further two from a patas monkey (Erythrocebus patas) and vervet monkey (Chlorocebus aethiops). The isolates were identified as F. tularensis ssp. holarctica on the basis of culture, morphological and biochemical characteristics. The identification was verified by polymerase chain reaction and the sequencing of the partial 16S rRNA gene. Utilization of carbon sources of the 15 F. tularensis strains was characterized with the Biolog system. The system was able to identify the strains already after 4 h of incubation, not only after the standard 24 h. After the analysis and comparison of the metabolic profiles of our strains with the Biolog database, it was concluded that not all carbon sources indicated in the database were utilized by our isolates. The Biolog software fails to distinguish the highly virulent F. tularensis ssp. tularensis and the moderately virulent F. tularensis ssp. holarctica but the Biolog microplates can be manually read to differentiate the two subspecies based on glycerol source utilization. As all the studied strains were unable to use glycerol, they could be identified as F. tularensis ssp. holarctica. The dendrogram based on the metabolic relationship of the strains shows that the isolates are very similar to each other, which correlates with the conservative genetic character of F. tularensis ssp. holarctica.


Subject(s)
Carbon/metabolism , Francisella tularensis/isolation & purification , Francisella tularensis/metabolism , RNA, Ribosomal, 16S/genetics , Tularemia/diagnosis , Animals , Chlorocebus aethiops , DNA Fingerprinting , Erythrocebus patas , Francisella tularensis/classification , Francisella tularensis/genetics , Hares/microbiology , Hungary , Polymerase Chain Reaction/veterinary , Sequence Analysis, DNA , Species Specificity , Tularemia/microbiology , Tularemia/veterinary , Virulence/genetics
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