Poly(3-hydroxybutyrate) production for food packaging from biomass derived carbohydrates by cupriavidus necator DSM 545.

The extensive utilization of conventional plastics has resulted in a concerning surge in waste. A potential solution lies in biodegradable polymers mostly derived from renewable sources. Cupriavidus necator DSM 545 is a microorganism capable, under stress conditions, of intracellularly accumulating Poly(3-hydroxybutyrate) (PHB), a bio-polyester. This study aimed to identify optimal conditions to maximize the intracellular accumulation of PHB and its global production using natural media obtained by processing lignocellulosic residues of cardoon, a low-cost feedstock. An intracellular PHB accumulation was observed in all of the tested media, indicating a metabolic stress induced by the lack of macronutrients. Increasing C/N ratios led to a significant decrease in cellular biomass and PHB production. Furthermore C. necator DSM 545 was incapable of consuming more than 25 g/L of supplied monosaccharides. Surprisingly, in the samples supplied with 60 % of the pentose-rich liquid fraction, complete consumption of xylose was observed. This result was also confirmed by subsequent tests using Medium 1 growth media containing xylose as the sole carbon source. Using a diluted medium with a C/N ratio of 5, a PHB production of 5.84 g/L and intracellular PHB accumulation of 77 % w/w were respectively achieved. Finally, comparative shelf-life tests conducted against conventional pre-packaging materials in PP suggested that PHB films performed similarly in preserve ready-to-eat products.

Wood waste valorization: Ethanol based organosolv as a promising recycling process.

Wood waste is a valuable material that could constitute an abundant and inexpensive source for the production of new materials the recovery of energy. In Europe, about 46% of wood waste is recycled to particleboard and fiberboard, while the other fraction is incinerated. However, a considerable quantity of wood waste shows potential for its transformation into value-added products due to its compositional quality. In this work, wood waste collected at a mechanical treatment plant underwent organosolv treatment to produce a cellulose pulp suitable for manufacturing containerboard. Three variables (temperature, acid concentration, and ethanol concentration) were investigated to find an optimal solution to produce wood pulp by means of Design of Experiment. Wood waste was microwave-heated at 160 °C for 15 min using an acidified ethanol-water solution (2% w/w H2SO4 and 0.8 w/w ethanol concentration), producing pulp with an average cellulose content of 76% where 93% of initial cellulose was retained. Thanks to a one-pot approach, ethanol was totally recovered, 62% of initial lignin was precipitated, and 20 g/l of hemicellulose-derived sugars solution was obtained. Finally, three wood waste samples collected in different periods of the year yielded comparable outcomes, suggesting a good reproducibility of the organosolv process. ANOVA test with a significance level of 0.01 showed a p-value of 0.029 and 0.235 for cellulose content and cellulose recovery, respectively. This study paves the way for an industrial symbiosis between recycling centers and paper mills located in the same territory.