Early postnatal overnutrition impairs VO2max gains with moderate exercise and increase post-exercise muscle damage in adult male rats.

Exercise counteracts obesity effects, but information on how early-life obesity may affect long-term adaptation to exercise is lacking. This study investigates the impact of early-life postnatal overfeeding (PO) on animals' adaptation to exercise. Only male Wistar rats were used. On postnatal day (PN) 30, rats from control (NL-9 pups) or PO (SL-3 pups) litters were separated into four groups: NL-sedentary (NL-Se), NL-exercised (NL-Ex), SL-sedentary (SL-Se), and SL-exercised (SL-Ex). Exercised groups performed moderate-intensity exercise, running on a treadmill, from PN30 to PN90. Further experiments were carried out between PN90 and PN92. PO promoted obesity in SL versus NL rats (P < 0.05). Exercise reduced body weight (P < 0.001), body fat (P < 0.01), and improved glucose homeostasis in SL-Ex versus SL-Se. SL-Ex presented lower VO2max (P < 0.01) and higher post-exercise LDH (P < 0.05) compared to NL-Ex rats. Although moderate exercise counteracted obesity in SL rats, early-life overnutrition restricts fitness gains in adulthood, indicating that early obesity may impair animals' adaptation to exercise.

Diagnósticos de enfermagem mais prevalentes à pacientes em perioperatório de neoplasia de cabeça e pescoço / Nursing diagnoses more prevalent to patients in perioperatory neoplasia of head and neck

Objetivo: Descrever a elaboração de uma tecnologia sobre diagnósticos de enfermagem mais prevalentes, segundo a taxonomia da NANDA International Inc. (NANDA-I), em pacientes perioperatório de cabeça e pescoço. Método:Estudo descritivo observacional, com abordagem qualitativa, do tipo Relato de Experiência, que descreve a atividade de residentes de Enfermagem no processo de elaboração de um produto de consulta adaptado sobre diagnósticos de enfermagem mais prevalentes em pacientes perioperatório de cirurgia de cabeça e pescoço. Resultados:O informativo foi dividido em domínios, conforme o exemplar NANDA-I, os tópicos foram definidos a partir de um diagnóstico situacional. Os itens que entraram para o conteúdo do impresso foram: I) Promoção da saúde, II) Nutrição III) Eliminação e Troca, IV) Atividade e repouso, V) Percepção/Cognição, VI) Auto-percepção, VII) papeis e relacionamento, VIII) Enfrentamento / tolerância ao estresse, IX) Segurança/proteção e X) Conforto. No final do impresso, fora inserido um QR code, com um linkdireto para o NANDA-I (2018-2021). Considerações finais:O estabelecimento de diagnósticos de enfermagem perpassa por um processo crítico, reflexivo que demanda conhecimento com base científica dos profissionais, que impactam na qualidade de vida que os usuários terão. A elaboração do produto sobre os principais diagnósticos na clínica de cabeça e pescoço do referido hospital, pelos residentes, demonstrou-se como uma estratégia de enorme potencial para orientação e organização das ações e serviços da enfermagem.

Objective: To describe the development of a technology on nursing diagnoses more prevalent, according to the taxonomy of NANDA International Inc. (NANDA-I) in head and neck perioperative patients. Method:Descriptive observational study, with qualitative approach, of the type Experience Report, which describes the activity of nursing residents in the process of elaboration of an adapted consultation product on nursing diagnoses more prevalent in perioperative patients of head and neck surgery. Results:The information was divided into domains, according to the NANDA-I copy, the topics were defined from a situational diagnosis. The items that entered the contents of the form were: I) Health promotion, II) Nutrition III) Elimination and Exchange, IV) Activity and rest, V) Perception/Cognition, VI) Self-perception, VII) roles and relationship, VIII) Coping / stress tolerance, IX) Safety/protection and X) Comfort. At the end of the form, a QR code was inserted, with a direct link to the NANDA-I (2018-2021). Final considerations:The establishment of nursing diagnoses permeates a critical, reflective process that demands knowledge based on the scientific basis of professionals, which impact on the quality of life that users will have. The elaboration of the product on the main diagnoses in the head and neck clinic of the referred hospital, by the residents, proved to be a strategy of enormous potential for guidance and organization of nursing actions and services.

Malnutrition during late pregnancy exacerbates high-fat-diet-induced metabolic dysfunction associated with lower sympathetic nerve tonus in adult rat offspring.

Objectives: We aimed to assess the effects of a maternal protein-caloric restriction diet during late pregnancy on the metabolism of rat offspring fed a high-fat diet (HFD) during adulthood.Methods: During late pregnancy, rat dams received either a low-protein (4%; LP group) or normoprotein (23%; NP group) diet. After weaning, the offspring were fed a standard diet (Control; C). Male offspring (60 days old) from both groups were then fed either the C diet or HFD until they were 90 days old. The adult offspring and maternal metabolic parameters and autonomic nervous system (ANS) were then evaluated.Results: Dams exhibited low body weight gain and food intake during the LP diet consumption. At lactation, these dams showed high body weight gain, hypoinsulinemia and hyperglycemia. The maternal LP diet resulted in low body weights for the pups. There were also no differences in the metabolic parameters between the adult LP offspring that were fed the C diet and the NP group. Adults of both groups that were fed the HFD developed obesity associated with altered insulin/ glucose homeostasis and altered ANS activity; however, the magnitudes of these parameters were higher in the LP group than in the NP group.Conclusions: Maternal protein malnutrition during the last third of pregnancy malprograms the metabolism of rat offspring, resulting in increased vulnerability to HFD-induced obesity, and the correlated metabolic impairment might be associated with lower sympathetic nerve activity in adulthood.

Aerobic Exercise Training Attenuates Tumor Growth and Reduces Insulin Secretion in Walker 256 Tumor-Bearing Rats.

Aerobic exercise training can improve insulin sensitivity in many tissues; however, the relationship among exercise, insulin, and cancer cell growth is unclear. We tested the hypothesis that aerobic exercise training begun during adolescence can attenuate Walker 256 tumor growth in adult rats and alter insulin secretion. Thirty-day-old male Wistar rats engaged in treadmill running for 8 weeks, 3 days/week, 44 min/day, at 55-65% VO2max until they were 90 days old (TC, Trained Control). An equivalently aged group was kept inactive during the same period (SC, Sedentary Control). Then, half the animals of the SC and TC groups were reserved as the control condition and the other half were inoculated with Walker 256 cancer cells, yielding two additional groups (Sedentary Walker and Trained Walker). Zero mortalities were observed in tumor-bearing rats. Body weight (BW), food intake, plasma glucose, insulin levels, and peripheral insulin sensitivity were analyzed before and after tumor cell inoculation. We also evaluated tumor growth, metastasis and cachexia. Isolated pancreatic islets secretory activity was analyzed. In addition, we evaluated mechanic sensibility. Our results showed improved physical performance according to the final workload and VO2max and reduced BW in trained rats at the end of the running protocol. Chronic adaptation to the aerobic exercise training decreased tumor weight, cachexia and metastasis and were associated with low glucose and insulin levels and high insulin sensitivity before and after tumor cell inoculation. Aerobic exercise started at young age also reduced pancreatic islet insulin content and insulin secretion in response to a glucose stimulus, without impairing islet morphology in trained rats. Walker 256 tumor-bearing sedentary rats also presented reduced pancreatic islet insulin content, without changing insulin secretion through isolated pancreatic islets. The mechanical sensitivity test indicated that aerobic exercise training did not cause injury or trigger inflammatory processes prior to tumor cell inoculation. Taken together, the current study suggests that aerobic exercise training applied during adolescence may mitigate tumor growth and related disorders in Walker 256 tumor-bearing adult rats. Improved insulin sensibility, lower glucose and insulin levels and/or reduced insulin secretion stimulated by glucose may be implicated in this tumor attenuation.

Aerobic exercise training performed by parents reduces mice offspring adiposity.

The present study aimed to determine the effects of physical training performed by parents on mice offspring adiposity. Male and female parents underwent an aerobic training protocol for 7 weeks. The trained and sedentary parents were allowed to mate and the resultant offspring divided in: S (Offspring from Sedentary Parents), T (Offspring from Trained Parents), ST (Offspring from Sedentary Father and Trained Mother) and TS (Offspring from Trained Father and Sedentary Mother). After weaning, offspring was euthanized, blood collected and samples of mesenteric and inguinal fat pads used to isolate adipocytes for morphologic and histological analyses. Lee index, mesenteric fat pad, sum of visceral fat and total fat weight of female T was reduced in comparison to the other groups (p < 0.05). Periepididymal and sum of visceral fat in male T group was also reduced when compared to the other groups (p < 0.05). The diameter of mesenteric and inguinal adipocytes of T group was smaller compared to all groups comparisons for both sexes (p < 0.05). In summary, exercise training performed by parents reduced visceral offspring adiposity, the diameter of subcutaneous adipocytes and improved metabolic parameters associated to metabolic syndrome.

Maternal low intensity physical exercise prevents obesity in offspring rats exposed to early overnutrition.

Low intensity exercise during pregnancy and lactation may create a protective effect against the development of obesity in offspring exposed to overnutrition in early life. To test these hypotheses, pregnant rats were randomly assigned into 2 groups: Sedentary and Exercised, low intensity, on a rodent treadmill at 30% VO2Max /30-minute/session/3x/week throughout pregnancy and the lactation. Male offspring were raised in small litters (SL, 3 pups/dam) and normal litters (NL, 9 pups/dam) as models of early overnutrition and normal feed, respectively. Exercised mothers showed low mesenteric fat pad stores and fasting glucose and improved glucose-insulin tolerance, VO2max during lactation and sympathetic activity. Moreover, the breast milk contained elevated levels of insulin. In addition, SL of sedentary mothers presented metabolic dysfunction and glucose and insulin intolerance and were hyperglycemic and hyperinsulinemic in adulthood. SL of exercised mothers showed lower fat tissue accretion and improvements in glucose tolerance, insulin sensitivity, insulinemia and glycemia. The results suggest that maternal exercise during the perinatal period can have a possible reprogramming effect to prevent metabolic dysfunction in adult rat offspring exposed to early overnutrition, which may be associated with the improvement in maternal health caused by exercise.

Acephate exposure during a perinatal life program to type 2 diabetes.

Acephate has been used extensively as an insecticide in agriculture. Its downstream sequelae are associated with hyperglycemia, lipid metabolism dysfunction, DNA damage, and cancer, which are rapidly growing epidemics and which lead to increased morbidity and mortality rates and soaring health-care costs. Developing interventions will require a comprehensive understanding of which excess insecticides during perinatal life can cause insulin resistance and type 2 diabetes. A Wistar rat animal model suggests that acephate exposure during pregnancy and lactation causes alterations in maternal glucose metabolism and programs the offspring to be susceptible to type 2 diabetes at adulthood. Therapeutic approaches based on preventive actions to food contaminated with insecticides during pregnancy and lactation could prevent new cases of type 2 diabetes.

Environmental Contaminants and Pancreatic Beta-Cells.

Despite health policies as well as clinical and research efforts, diabetes prevalence is still rising around the world. A multitude of causes have been suggested for this increase, mostly related to familial background, the occidental diet which is rich in fat/carbohydrates, and sedentary life style. Type 2 diabetes involves malfunctions of the primary pancreatic beta-cells, usually attributed to local damage; however, it can be associated with other stressful environmental agents, such as chemical contaminants from food, plastic and air, among others. Indeed, exposure to these chemical agents during perinatal and adolescent life can increase the risk of developing cardiometabolic diseases later in life. This review explores data showing which environmental chemical agents may produce injury in beta-cells and further impair the insulinotropic process of type 2 diabetes. Additionally, it points the need to also consider unusual causes of metabolic diseases, such as environmental contaminants.

Maternal Diet Supplementation with n-6/n-3 Essential Fatty Acids in a 1.2 : 1.0 Ratio Attenuates Metabolic Dysfunction in MSG-Induced Obese Mice.

Essential polyunsaturated fatty acids (PUFAs) prevent cardiometabolic diseases. We aimed to study whether a diet supplemented with a mixture of n-6/n-3 PUFAs, during perinatal life, attenuates outcomes of long-term metabolic dysfunction in prediabetic and obese mice. Seventy-day-old virgin female mice were mated. From the conception day, dams were fed a diet supplemented with sunflower oil and flaxseed powder (containing an n-6/n-3 PUFAs ratio of 1.2 : 1.0) throughout pregnancy and lactation, while control dams received a commercial diet. Newborn mice were treated with monosodium L-glutamate (MSG, 4 mg g-1 body weight per day) for the first 5 days of age. A batch of weaned pups was sacrificed to quantify the brain and pancreas total lipids; another batch were fed a commercial diet until 90 days of age, where glucose homeostasis and glucose-induced insulin secretion (GIIS) as well as retroperitoneal fat and Lee index were assessed. MSG-treated mice developed obesity, glucose intolerance, insulin resistance, pancreatic islet dysfunction, and higher fat stores. Maternal flaxseed diet-supplementation decreased n-6/n-3 PUFAs ratio in the brain and pancreas and blocked glucose intolerance, insulin resistance, GIIS impairment, and obesity development. The n-6/n-3 essential PUFAs in a ratio of 1.2 : 1.0 supplemented in maternal diet during pregnancy and lactation prevent metabolic dysfunction in MSG-obesity model.

Early treatment with metformin induces resistance against tumor growth in adult rats.

It is known that antidiabetic drug metformin, which is used worldwide, has anti-cancer effects and can be used to prevent cancer growth. We tested the hypothesis that tumor cell growth can be inhibited by early treatment with metformin. For this purpose, adult rats chronically treated with metformin in adolescence or in adulthood were inoculated with Walker 256 carcinoma cells. Adult rats that were treated with metformin during adolescence presented inhibition of tumor growth, and animals that were treated during adult life did not demonstrate any changes in tumor growth. Although we do not have data to disclose a molecular mechanism to the preventive metformin effect, we present, for the first time, results showing that cancer growth in adult life is dependent on early life intervention, thus supporting a new therapeutic prevention for cancer.

Short-term moderate exercise provides long-lasting protective effects against metabolic dysfunction in rats fed a high-fat diet.

INTRODUCTION: A sedentary lifestyle and high-fat feeding are risk factors for cardiometabolic disorders. This study determined whether moderate exercise training prevents the cardiometabolic changes induced by a high-fat diet (HFD). MATERIALS AND METHODS: Sixty-day-old rats were subjected to moderate exercise three times a week for 30 days. After that, trained rats received a HFD (EXE-HFD) or a commercial normal diet (EXE-NFD) for 30 more days. Sedentary animals also received the diets (SED-HFD and SED-NFD). Food intake and body weight were measured weekly. After 120 days of life, analyses were performed. Data were analysed with two-way ANOVA and the Tukey post-test. RESULTS: Body weight gain induced by HFD was attenuated in trained animals. HFD reduced food intake by approximately 30% and increased body fat stores by approximately 75%. Exercise attenuated 80% of the increase in fat pads and increased 24% of soleus muscle mass in NFD animals. HFD induced a hyper-response to glucose injection, and exercise attenuated this response by 50%. Blood pressure was increased by HFD, and the beneficial effect of exercise in reducing blood pressure was inhibited by HFD. HFD increased vagal activity by 65% in SED-HFD compared with SED-NFD rats, and exercise blocked this increase. HFD reduced sympathetic activity and inhibited the beneficial effect of exercise on ameliorating sympathetic activity. CONCLUSION: Four weeks of moderate exercise at low frequency was able to prevent the metabolic changes induced by a HFD but not the deleterious effects of diet on the cardiovascular system.

Low-intensity and moderate exercise training improves autonomic nervous system activity imbalanced by postnatal early overfeeding in rats.

BACKGROUND: Postnatal early overfeeding and physical inactivity are serious risk factors for obesity. Physical activity enhances energy expenditure and consumes fat stocks, thereby decreasing body weight (bw). This study aimed to examine whether low-intensity and moderate exercise training in different post-weaning stages of life is capable of modulating the autonomic nervous system (ANS) activity and inhibiting perinatal overfeeding-induced obesity in rats. METHODS: The obesity-promoting regimen was begun two days after birth when the litter size was adjusted to 3 pups (small litter, SL) or to 9 pups (normal litter, NL). The rats were organized into exercised groups as follows: from weaning until 90-day-old, from weaning until 50-day-old, or from 60- until 90-days-old. All experimental procedures were performed just one day after the exercise training protocol. RESULTS: The SL-no-exercised (SL-N-EXE) group exhibited excess weight and increased fat accumulation. We also observed fasting hyperglycemia and glucose intolerance in these rats. In addition, the SL-N-EXE group exhibited an increase in the vagus nerve firing rate, whereas the firing of the greater splanchnic nerve was not altered. Independent of the timing of exercise and the age of the rats, exercise training was able to significantly blocks obesity onset in the SL rats; even SL animals whose exercise training was stopped at the end of puberty, exhibited resistance to obesity progression. Fasting glycemia was maintained normal in all SL rats that underwent the exercise training, independent of the period. These results demonstrate that moderate exercise, regardless of the time of onset, is capable on improve the vagus nerves imbalanced tonus and blocks the onset of early overfeeding-induced obesity. CONCLUSIONS: Low-intensity and moderate exercise training can promote the maintenance of glucose homeostasis, reduces the large fat pad stores associated to improvement of the ANS activity in adult rats that were obesity-programmed by early overfeeding.

Maternal diet, bioactive molecules, and exercising as reprogramming tools of metabolic programming.

Nutrition and lifestyle, particularly over-nutrition and lack of exercise, promote the progression and pathogenesis of obesity and metabolic diseases. Nutrition is likely the most important environmental factor that modulates the expression of genes involved in metabolic pathways and a variety of phenotypes associated with obesity and diabetes. During pregnancy, diet is a major factor that influences the organ developmental plasticity of the foetus. Experimental evidence shows that nutritional factors, including energy, fatty acids, protein, micronutrients, and folate, affect various aspects of metabolic programming. Different epigenetic mechanisms that are elicited by bioactive factors in early critical developmental ages affect the susceptibility to several diseases in adulthood. The beneficial effects promoted by exercise training are well recognised, and physical exercise may be considered one of the more prominent non-pharmacological tools that can be used to attenuate metabolic programming and to consequently ameliorate the illness provoked by metabolic diseases and reduce the prevalence of obesity, type 2 diabetes, and cardiovascular diseases. Literature on the different outcomes of unbalanced diets and the beneficial effects of some bioactive molecules during gestation and lactation on the metabolic health of offspring, as well as the potential mechanisms underlying these effects, was reviewed. The importance of the combined effects of functional nutrition and exercise as reprogramming tools of metabolic programming is discussed in depth. Finally, this review provides recommendations to healthcare providers that may aid in the control of early programming in an attempt to optimise the health of the mother and child.

Early overfeed-induced obesity leads to brown adipose tissue hypoactivity in rats.

BACKGROUND/AIMS: Brown adipose tissue activation has been considered a potential anti-obesity mechanism because it is able to expend energy through thermogenesis. In contrast, white adipose tissue stores energy, contributing to obesity. We investigated whether the early programming of obesity by overfeeding during lactation changes structure of interscapular brown adipose tissue in adulthood and its effects on thermogenesis. METHODS: Birth of litters was considered day 0. On day 2, litter size was adjusted to normal (9 pups) and small (3 pups) litters. On day 21, the litters were weaned. A temperature transponder was implanted underneath interscapular brown adipose tissue pads of 81-day-old animals; local temperature was measured during light and dark periods between days 87 and 90. The animals were euthanized, and tissue and blood samples were collected for further analysis. The vagus and retroperitoneal sympathetic nerve activity was recorded. RESULTS: Small litter rats presented significant lower interscapular brown adipose tissue temperature during the light (NL 37.6°C vs. SL 37.2°C) and dark (NL 38°C vs. SL 37.6°C) periods compared to controls. Morphology of small litter brown adipose tissue showed fewer lipid droplets in the tissue center and more and larger in the periphery. The activity of vagus nerve was 19,9% greater in the small litter than in control (p<0.01), and no difference was observed in the sympathetic nerve activity. In adulthood, the small litter rats were 11,7% heavier than the controls and presented higher glycemia 13,1%, insulinemia 70% and corticosteronemia 92,6%. CONCLUSION: Early overfeeding programming of obesity changes the interscapular brown adipose tissue structure in adulthood, leading to local thermogenesis hypoactivity, which may contribute to obesity in adults.

Efeito da redução de ninhada sobre as respostas autonômicas e metabólicas de ratos Wistar / Effect of small litter size on the autonomic and metabolic responses of Wistar rats

OBJETIVO: Este estudo investigou o perfil lipídico e a atividade elétrica dos nervos parassimpático (vago superior) e simpático (localizado na região esplâncnica) de ratos obesos oriundos de ninhada reduzida. MÉTODOS: Foram pesquisados dois grupos distintos, com 12 animas cada um: ninhada padrão, padronizado em nove filhotes por ninhada, e ninhada reduzida, três filhotes por ninhada. O consumo de ração e peso corporal foi acompanhado do desmame até o final do protocolo experimental. Aos 90 dias de idade, os animais foram anestesiados com (Thiopental®) e submetidos ao registro da atividade elétrica dos nervos simpático (vago) e parassimpático (da região esplâncnica); em seguida, foram sacrificados e retiradas e pesadas as gorduras retroperitoneal e periepididimal. Amostras de sangue foram coletadas para dosagens de glicemia, insulinemia, colesterol total, triglicerídeos e lipoproteína de alta densidade colesterol. RESULTADOS: Os ratos de ninhada reduzida apresentaram aumento da ingestão alimentar, peso corporal e tecido adiposo branco, quadros de hiperglicemia, hiperinsulinemia e hipercolesterolemia, aumento dos triglicérides e redução do lipoproteína de alta densidade colesterol. CONCLUSÃO: Quanto à atividade do nervo vago, os ratos ninhada reduzida apresentaram um aumento significativo em relação aos ratos ninhada padrão, e mesmo não havendo diferença na atividade simpática, o modelo ninhada reduzida mostrou-se eficaz para indução da obesidade, dislipidemia, hipercolesterolemia, hiperinsulinemia, hiperglicemia e desequilíbrio autonômico em roedores.

OBJECTIVE: This study investigated the lipid profile and electric activity of the parasympathetic (vagus nerve) and sympathetic (located in the splanchnic region) nerves of obese rats from small litters. METHODS: Two distinct groups were studied, each with 12 animals: normal litter with nine pups per litter and small litter, with three pups per litter. Chow intake and body weight were monitored from weaning until the end of the experimental protocol. At age 90 days, the animals were anesthetized with Thiopental® for investigation of the electric activity of the sympathetic and parasympathetic nerves. They were then sacrificed for removal and weighing of the retroperitoneal and epididymal fat pads. Blood samples were collected for determination of blood glucose, insulin, total cholesterol, triglycerides and high-density lipoprotein cholesterol. RESULTS: The small litters rats had high food intake, body weight, white fat tissue, blood glucose, blood insulin, total cholesterol and triglycerides, and low high-density lipoprotein cholesterol levels. CONCLUSION: The vagus nerve of the small litters rats was significantly more active than that of the normal litter rats. Sympathetic activity did not differ between the groups but the small litters model effectively promoted obesity, dyslipidemia, hypercholesterolemia, hyperinsulinemia, hyperglycemia and autonomic imbalance in rats.

Hábitos alimentares, níveis de atividade física e perfil antropométrico de vigilantes da Universidade Estadual de Maringá de diferentes turnos de trabalho / Food habits, physical activity levels and anthropometric profile of watchmen of different work shift from State University of Maringá

Este trabalho objetivou investigar o impacto que os diferentes turnos de trabalho exercem sobre o estilo de vida de vigilantes. Participaram do estudo 30 vigilantes do sexo masculino, de três turnos diferentes. Os hábitos alimentares foram avaliados pelo inquérito alimentar (recordatório mensal). A atividade física foi avaliada por meio do IPAQ - versão curta. O perfil antropométrico foi verificado pelo peso corporal e estatura, (IMC) e percentual de gordura. Para verificar a distribuição dos dados aplicou-se o teste de Shapiro Wilk. Para comparação dos grupos quanto às variáveis quantitativas, utilizou-se o teste Anova 1 fator. Para verificar possíveis associações entre o turno de trabalho e as variáveis dependentes utilizou-se o Qui-quadrado de Pearson e o Qui-quadrado para tendência. Não foram encontradas diferenças significativas entre as variáveis investigadas de acordo com o turno de trabalho (p>0,05), assim como, não houve associação do turno de trabalho com o nível de atividade física (p=0,98), com os hábitos alimentares (p=0,42) e com a gordura (p=0,73). Dessa forma, conclui-se que o turno de trabalho não causou impacto no estilo de vida dos vigilantes, principalmente os do turno noturno, o que se sugere que novos estudos sejam realizados em outras instituições de ensino, uma vez que a realidade da UEM pode não se repetir.

This study aimed to investigate the impact that different work shifts had on guards? lifestyle. A total of 30 watchmen from three different shifts took part in the study. Nutritional statuses were evaluated through diet surveys (monthly recording). Physical activity was evaluated using IPAQ ? short version. Anthropometric profiles were verified through weight and height (BMI) and fat percentage. The Shapiro-Wilk test was applied for data analysis. One-way ANOVA was used to compare the quantitative variables of groups. To investigate possible associations between work shifts and dependent variables, Pearson?s chi-square and the chi-square for trend were applied. Significant differences were not found between any of the investigate variables according to the work shifts (p>0,05), as same as there were no association between work shifts and physical activity level (p=0,98) with food habits (p=0,42) and fat percentage (p=0,73). Therefore it can be concluded that work shift had no impact at guards? lifestyle, especially those in the night shift, which suggests that further studies should be performed in other educational institutions since the UEM setting cannot be repeated.