Antitumor and immunomodulating effects of a fragment of HL-60 cell differentiation factor in mice with lewis pulmonary carcinoma.

A six-member HLDF6 peptide, fragment of HL-60 cell differentiation factor, exhibited antimetastatic and immunomodulating effects.

[Effects of the dsRNA interferon inducer on the interaction between macrophages and Mycobacterium tuberculosis in vitro]. / Vliianie induktora interferona na osnove dvuspiral'noi RNK n vzaimodeistvie makrofagove i Mycobacterium tuberculosis in vitro.

Effects of the IFN inducer, yeast dsRNA, produced on the interaction between mouse macrophages and phagocytized mycobacteria were experimentally studied in vitro. Mycobacteria were shown to reproduce in macrophages in their initial infection at a ratio of 1:1.25, 1:2.5, 1:5 and 1:10, which was confirmed by an increased insertion of 5.6-[3H]-uracil in M. tuberculosis H37Rv; they also had a destructive impact on macrophages as verified by a higher release of lactic dehydrogenase (LDG) from macrophages. The dsRNA preparation, 40.0, 80.0 and 120.0 microg/ml, was demonstrated to decrease the insertion amount of labeled uracil in phagocytized mycobacteria at a macrophage:mycobacteria ratio of 1:10 and 1:100. The effect depended on a preparation dose and infection degree of macrophages. A decreased release of specific LDG from infected macrophages was shown under the same conditions. The dsRNA affects the interplay of macrophages and phagocytized mycobacteria through inhibiting the vitality of intracellular mycobacteria and through enhancing the stability of macrophages. Special studies denoted that dsRNA, 40.0 microg/ml and 120 microg/ml, activated the production of peroxidation compounds by neutrophils, which phagocytized the sheep erythrocytes. Finally, a possible mechanism of dsRNA impact on the interaction between macrophages and mycobacteria phagocytized by them is under discussion.

[The impact of compound of double-stranded RNA and hyaluronic acid on the parameters of non-specific resistance in mice]. / Vliianie kompozitsii dvuspiral'nykh RNK i gialuronovoi kisloty na pokazateli nespetsificheskoi ustoichivosti myshei.

The effect of yeast double-stranded RNA (dsRNA) and of hyaluronic acid (HA) compositions produced on the interferon synthesis, peritoneal phagocytic activity of macrophages and on the hematological parameters were studied in an experiment with white noninbred mice. HA was shown to enhance the dsRNA-induced interferon synthesis, to inhibit the leukopenic reaction and to produce no effect on the phagocytosis-stimulating activity. The data obtained are indicative of that HA is a promising preparation regarding its use within the interferon-inducing compositions based on dsRNA.

[Study of the antiviral activity, pharmacokinetics and toxic properties of liposomal form of human alpha-2b interferon administered topically]. / Izuchenie protivovirusnoi aktivnosti, farmakokinetiki i toksicheskikh svoistv liposomal'noi formy rekombinantnogo alfa-2b-interferona cheloveka pri naruzhnom primenenii.

Human liposomal recombinant alpha 2b-interferon topically applied to laboratory animals was tested for antiviral activity, pharmacokinetics, and toxicity. The interferon was shown to penetrate through the skin and to circulate in the blood of experimental animals longer than its injectable form, and to exhibit its antiviral activity against genital herpes in guinea pigs. It produced no toxic, skin-irritant, or allergic effects in laboratory animals.

Potentiation of antitumor effects of cisplatin by tumor necrosis factor-beta.

Recombinant tumor necrosis factor-beta potentiated the inhibitory effect of cisplatin on intramuscularly transplanted GA-1 tumor and liver metastasis in mice. The antitumor effect was related to cytotoxic and cytostatic activities of the test preparations rather than to initiation of apoptosis.

[The activation of phagocytosis by recombinant human tumor necrosis factor-beta]. / Aktivatsiia fagotsitoza rekombinantnym chelovecheskim faktorom nekroza opukholi beta.

The functional activity of phagocytic cells of various types was studied in white non-inbred mice by administering recombinant human tumor necrosis beta (rhTNF-beta). It was shown that rhTNF-beta increased phagocytic activity of the peritoneal exudate, spleen and liver macrophages as well as blood polynuclears. Stimulation of neutrophils was demonstrated in earlier times after administration of the preparation as compared to macrophages (3 h and 24 h, respectively). The duration of the macrophage activation effect and its expression depended on the dose of the preparation and were the most notable when rhTNF-beta was administered in doses of 10(3)-10(5) U/20 g. The addition of reopolyglucin, the polysaccharide filler, didn't remove a stimulatory effect of rhTNF-beta on macrophages, but influenced its dynamics. Multiple administration of the preparation didn't cause the phagocytosis stimulation effect.

[The immunoregulatory properties of recombinant human tumor necrosis factor-beta in mice opposite-reacting to antigen]. / Issledovanie immunoreguliatornykh svoistv rekombinantnogo faktora nekroza opukholi beta cheloveka u oppozitnoreagiruiushchikh na antigen myshei.

The effect of 10(3)-10(5) E/20 g doses of the recombinant factor of human beta tumor necrosis (rFNT-beta) on formation of the immune response and macrophage functional activity was studied in CBA and C57Bl/6 mice that differ in genetically determined level of the immune response to an antigen (sheep erythrocytes). The rFNT-beta was found to cause a modulating effect on the cell and humoral links of the immune response. The effect of the agent depended on the dose and the genotype of the experimental animals. It is suggested that the interlinear differences in the intensity of the humoral immune response in rFNT-beta administration may be connected with the different sensitivity to the agent of the peritoneal macrophages of mice of the used lines.

[Features of formation of antiviral resistance upon local application of the interferon inducer ridostin]. / Osobennosti formirovaniia protivovirusnoi ustoichivosti pri mestnom primenenii induktora interferona ridostina.

Antiviral potency of an ointment dosage form of interferon (IF) inducer ridostin has been demonstrated in guinea pigs with genital herpes. Early beginning of therapy led to a 72% decrease of the intensity of clinical signs with cure 12-13 days earlier than in the control. Application of ridostin ointment onto the skin of intact animals caused an increase of IF level in the blood and of the phagocytic activity of peritoneal exudation macrophages. Ridostin effect on the total-systems factors of nonspecific defense appears to be one mechanism in the formation of antiviral resistance upon external application of this IF inducer.

[Study of immunoregulating properties of an interferon inducer in animals with various reactions to the antigen]. / Issledovanie immunoreguliatornykh svoistv induktora interferona u oppozitnoreagiruiushchikh na antigen zhivotnykh.

Influence of dsRNA isolated from killer yeast of S. cerevisiae on humoral and cellular immune responses in mice CBA/CaY and C57Bl/6Y with opposing reaction to the antigen was studied. It was shown that after administration of the yeast dsRNA preparation to the animals simultaneously with the antigen there was an increase in the number of the antibody forming cells in the spleen and the titer of hemolytic antibodies in blood serum of the animals with high and low reactions to the antigen. After sensitization with different doses of sheep red blood cells (10(7) and 10(8)) the preparation had immunomodulating action on development of DTH in mice CBA/CaY. The effect of the dsRNA preparation on the immunity system depended on the preparation dose, antigen loading and animal genotype and was the most marked in mice CBA/CaY with interferon levels in blood serum 2-3 times higher than those in mice C57Bl/6Y.

[Immunomodulating action of a preparation of yeast dsRNA on cellular immunity in mice]. / Izuchenie immunomoduliruiushchego deistviia preparata drozhzhevoi dsRNK na kletochnyi immunitet u myshei.

The effect of a dsRNA preparation (an interferon inductor) on DTH induced by SRBC was studied. It was shown that at optimal antigenic load the dsRNA preparation inhibited DTH whereas at suboptimal and supraoptimal loads the preparation stimulated it. The findings indicated that the dsRNA preparation had an immunoregulatory effect. The immunoregulatory properties of the preparation must be associated with its action on the lymphocyte suppressor cells and macrophages.

[Action of a yeast dsRNA preparation on the humoral immune response and the delayed hypersensitivity reaction]. / Issledovanie deistviia preparata drozhzhevoi dsRNK na gumoral'nyi immunnyi otvet i reaktsiiu giperchuvstvitel'nosti zamedlennogo tipa.

The studies showed that yeast dsRNA in a dose of 2.5 mg/kg had a stimulating effect on the humoral immune response. When the preparation was administered soon after sensitization of experimental animals by the thymus-dependent antigen (sheep red blood cells, SRBC), there were observed a more than two-fold increase in the AFC count in the spleen and increased titers of hemolysins in blood serum. With the use of higher doses of the preparation (up to 10 mg/kg) the level of the immunostimulating effect remained unchanged. The efficacy of the preparation in CBA and C57BL/6 mice with high and low reactivity to the antigen was the same. Delayed-type hypersensitivity in the animals sensitized by SRBC was inhibited by yeast dsRNA in a dose of 10 mg/kg.