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1.
Dtsch Med Wochenschr ; 119(1-2): 1-6, 1994 Jan 07.
Article in German | MEDLINE | ID: mdl-7506645

ABSTRACT

The value of measuring the urinary concentration of alpha 2-macroglobulin in addition to that of C-reactive protein (CRP) was assessed in a prospective study of 78 consecutive patients (29 women, 49 men; mean age 48.7 [19-75] years) after renal transplantation. alpha 2-Macroglobulin was never demonstrated in urine when the course was normal (n = 38), cytomegalovirus infection had occurred (n = 26) or acute cyclosporin nephrotoxicity (n = 5) or glomerular disease in the transplant (n = 10). CRP was present in only a few such cases. Interstitial rejections (n = 26) always led to urinary alpha 2-macroglobulin and CRP excretion without haematuria, while in vascular rejection (n = 3) the haemoglobin test was also positive. Urinary infection (n = 20) and urosepticaemia (n = 6) always brought about the urinary excretion of alpha 2-macroglobulin and CRP, as well as a usually highly positive haemoglobin test. alpha 2-Macroglobulin was absent but CRP always present in extrarenal bacterial infections (n = 30). Postrenal blood admixture was always characterized by a positive haemoglobin test and alpha 2-macroglobulin in urine, while in most cases (83%) CRP was absent. --These results indicate that the constellation "alpha 2-macroglobulin negative/CRP positive" is a pathognomonic for extrarenal bacterial infection (sensitivity 100%, specificity 98.9%). The presence of alpha 2-macroglobulin alone makes postrenal blood admixture probable. If both proteins are present in the urine, rejection and urogenital bacterial infection must be excluded by further tests.


Subject(s)
Bacterial Infections/diagnosis , Graft Rejection/diagnosis , Kidney Transplantation , Urinary Tract Infections/diagnosis , alpha-Macroglobulins/urine , Adult , Aged , C-Reactive Protein/urine , Cyclosporine/adverse effects , Cytomegalovirus Infections/diagnosis , Diagnosis, Differential , Female , Hematuria/diagnosis , Hemoglobinuria/diagnosis , Humans , Kidney/drug effects , Kidney Diseases/diagnosis , Male , Middle Aged , Prospective Studies , Sepsis/diagnosis , Sepsis/etiology , Urinary Tract Infections/complications
2.
Dtsch Med Wochenschr ; 117(23): 894-9, 1992 Jun 05.
Article in German | MEDLINE | ID: mdl-1597117

ABSTRACT

A prospective study was undertaken in 73 patients (24 women, 49 men; mean age 47.9 [21-64] years) after renal transplantation to discover whether the presence of C-reactive protein in urine (CRPu) and its serum concentration (CRPs) are of value in the differential diagnosis of abnormal function in the transplanted kidney. CRPu concentration was measured with a highly sensitive immunoluminometric assay (minimal threshold value 6 micrograms/l). CRPu was demonstrated in 36 histologically proven rejection episodes and 21 bacterial infections proven by culture. In contrast, no CRPu was demonstrated when the course was normal and in individual cases of cyclosporin renal toxicity, as well as in 27 of 34 cases of cytomegalovirus infection. In addition, the CRPs to CRPu ratio was a sensitive means of distinguishing between rejection (CRPs/CRPu less than 1) and bacterial infection (CRPs/CRPu greater than 1). Determining CRPu concentration thus proved to be useful in the initial monitoring of renal transplantation before starting any specific urinary protein diagnosis, as well as (together with CRPs) in the diagnosis of severe posttransplantation complications.


Subject(s)
C-Reactive Protein/urine , Kidney Diseases/diagnosis , Kidney Transplantation/physiology , Postoperative Complications/diagnosis , Adult , Diagnosis, Differential , Female , Graft Rejection , Humans , Immunologic Tests/methods , Kidney Diseases/urine , Male , Middle Aged , Postoperative Complications/urine , Prospective Studies , Sensitivity and Specificity
3.
Dtsch Med Wochenschr ; 115(26): 1014-7, 1990 Jun 29.
Article in German | MEDLINE | ID: mdl-2361438

ABSTRACT

24 days after starting treatment of psoriasis with fumaric acid derivatives (0.8-1.0 g orally, plus unknown quantities locally) a 21-year-old woman developed acute oliguric renal failure with a rise of serum creatinine levels to 1094 mumol/l (12.4 mg/dl). Deterioration of renal function had been preceded by severe abdominal symptoms with nausea, vomiting and colicky pain. On admission to hospital she was dehydrated with hyponatraemia and hypokalaemia. There was glomerular microhaematuria, increased excretion of renal epithelia, and tubular proteinuria. Renal biopsy demonstrated acute tubular damage with vacuolization of proximal epithelia, dilated tubules and scattered necroses. After intermittent haemodialysis (13 courses over two weeks) renal function gradually recovered, as demonstrated at a follow-up examination four months after discharge.


Subject(s)
Acute Kidney Injury/chemically induced , Fumarates/adverse effects , Psoriasis/drug therapy , Acute Kidney Injury/diagnosis , Acute Kidney Injury/pathology , Adult , Biopsy , Chronic Disease , Drug Combinations , Drug Therapy, Combination , Female , Fumarates/therapeutic use , Humans , Kidney/drug effects , Kidney/pathology , Powders , Psoriasis/complications
5.
Eur J Biochem ; 50(3): 549-55, 1975 Jan 15.
Article in English | MEDLINE | ID: mdl-234845

ABSTRACT

The polynucleotides poly[r(A-s-2U)] and poly]r(A-s2s4U)] have been synthesized and characterized by nearest-neighbour analysis, sedimentation analysis as well as spectroscopic techniques. Absorption-temperature profile and absorption-pH profile of poly[r(A-s-2U)] did not reveal a structural transition between 10 and 95 degrees C even at low ionic strength, although a variety of properties indicated a helical structure of poly[r(A-s-2U)]: remarkable hyperchromicity of the absorption spectrum, circular dichroic spectrum displaying extrema of large amplitudes, resistance against hydrolysis by ribonuclease and interaction with ethidium bromide in a manner which is characteristic of helical polynucleotides. Our results show that interactions of the type A-s-2U and A-s-2s-4U do in fact exist in helical polynucleotides. The properties of poly]r(As-2U)] furthermore demonstrate the general stabilizing effect of 2-thioketopyrimidine bases in helical polynucleotides by virtue of vertical stacking interactions with neighbouring pyrimiding and purine bases.


Subject(s)
Poly A-U/analogs & derivatives , Circular Dichroism , Ethidium/pharmacology , Hydrogen-Ion Concentration , Hydrolysis , Nucleic Acid Conformation , Nucleic Acid Denaturation , Osmolar Concentration , Ribonucleases/pharmacology , Spectrophotometry, Ultraviolet , Sulfur
15.
FEBS Lett ; 10(1): 41-45, 1970 Sep 18.
Article in English | MEDLINE | ID: mdl-11945352
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